Establishment of vascular prostheses using self endothelial precursor cell with gene modification

即使在冠状动脉旁路移植术（CABG）中，调节小血管假体的闭塞也是一个重要的问题。为此，我们设计了基因转移内皮细胞包被血管假体。基因转移的自身内皮细胞可防止血栓闭塞或血管狭窄与内皮细胞增殖及其致血栓性相关。我们利用外周内皮前体细胞研究内皮细胞增殖导致闭塞的机制。采用抗cd34抗体包被磁珠法从人外周血中获得内皮前体细胞。我们观察到CD34阳性的内皮前体细胞在牛脑提取物（内皮生长因子）的培养下发育为内皮细胞。反过来，我们利用基因枪建立了基因转移系统，实现了基因的高表达。因此，我们的下一个问题是将内皮前体细胞应用于血管假体。此外，我们将探讨基因枪基因转移是否有助于阻止小血管假体内皮细胞的增殖。

It is an important issue to regulate the occlusion of small sized vascular prostheses, that achieve constitutive patency even in coronary artery bypass grafting (CABG). For this issue, we designed gene transferred endothelial cells coated vascular prostheses. Gene transferred self endothelial cells will prevent thrombotic occlusion or vascular stenosis associated with the endothelial proliferation and its thrombogenicity.We investigated how the endothelial cell proliferation caused the occlusion using peripheral endothelial precursor cells. Endothelial precursor cells were obtained from human peripheral blood using the anti-CD34 antibody-coated magnet beads method. We observed that CD34 positive endothelial precursor cells developed to endothelial cells in the culture with bovine brain extract, which was endothelial growth factor. Conversely, we established gene transfer system using gene gun, that achieved high gene expression.Therefore we have the next issue to apply endothelial precursor cells onto the vascular prostheses. In addition, we are going to investigate whether or not gene transfer using gene gun is useful to prevent the endothelial cell proliferation in small sized vascular prostheses.