Establishment of in vitro culture system of seminiferous epitherium and clarifying the mechanism of spermatogenesis

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To establish the in vitro culture system of the seminiferous epitherium is an important research subject for clarifying the mechanisms of both the sexual differentiation and the spermatogenesis. In the present study, results were conipbsed of three parts, and all of them were published in 8scientific papers. First, we demonstrated the stage-specific appearance of glycoproteins and glycolipeds on the cell membrane of sertoli cells and germ cells in mouse testes. Using in vitro culture system, the role of these molecules was clarified. by adding the inhibitors against correspondinq moldcules, that is, the strong involvement of these molecules in the spermatogenesis. Second, to find outthe essential factors on spermatogenesis, morphological-characteristics of interstitial tissues was examined in the boar testis. The separation mechanism between the basal and abluminal compartments in the seminiferous epitherium was first indicated by observing the cytoskeleton in the ectoplasmic specialization (bloodtestis barrier) in boar testis by deep-eecth method. Further, monoclonal antibodies specific to-germ cells were cloned out in this study, ana a 103 KDa protein was detected in the cells from pachytene spermatocytes to early spermatids. Third, to clarify the counter-current transfer of substances from the testicular vein to the artery was morphologically examined using three kinds of mammals, and both the common and specific architectures were demonstrated in this study.