Cellular and molecular analysis of cryptic testes in dolphins

海豚隐睾的细胞和分子分析

• 批准号: 09460125
• 负责人: HAYASHI Yoshihiro
• 金额: $ 7.04万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09460125/
• 关键词: cryptic testis; Heat Shock Proteins; bottlenose dolphin; Risso's dolphin; spermatogenesis; lectin; spermatogenic cell; 精巣; 精上皮; 実験的腹腔内精巣; イルカ; 精細管

In many mammals, the testis is located in the scrotum and is maintained cooler than the deep body temperature. As a result, spermatogenesis is retained to be active. However, the testes in some mammals, such as dolphins, elephants and hyraxes, are present within the abdominal cavity. It remains to be unclear how they can maintain their spermatogenesis under this condition. The present study was designed to examine the relationship between the cryptic testis and the appearance/disappearance of heat shock proteins and lectins. In addition to dolphins, we used experimental cryptorchid mice, gonadally inactive hamster testes and mouse testes at fetal and early postnatal stages (prespermatogenesis). In experimental cryptorchidism of mice, many degenerating cells showed condensation of the nucleus and cytoplasm. TUNEL-positive cells were frequently observed. These data provide evidence that at least some of the cells die by apoptosis. While, hsp 70.2 was expressed in pachytene spermatocytes … More and spermatids. No reaction was detected in cryptorchid testes. In hamster testes, spermatogenesis was severely disrupted at 13 weeks after exposure to a short day cycle. Dolichos biflorus (DBA) agglutinin bound specifically to spermatogonia. DBA bound to only type A spermatogonia in active testes, whereas it bound to type A, intermediate and type B spermatogonia in inactive testes. During prespermatogenesis, characteristic binding patterns of sWGA, VVA and LEA agglutinin were observed in gonocytes. These lectins nay be related to the differentiation of gonocytes. In bottlenose dolphins, hsp86 was strongly positive in type B spermatogonia, spermatocytes and spermatids. Hsp84 was strongly expressed in type B spermatogonia, whereas no reaction was detected in spermatocytes and spermatids. In Risso's dolphins, hsp86 was expressed in pachytene and preleptotene spermatocytes. Hsp84 reacted with type B spermatogonia. These findings suggest that each protein may play a role in the different process of spermatogenesis. Less

在许多哺乳动物中，睾丸位于阴囊中，保持比身体深部温度低。因此，精子发生保持活跃。然而，一些哺乳动物的睾丸，如海豚、大象和蹄兔，存在于腹腔内。目前尚不清楚它们如何在这种情况下维持精子发生。本研究旨在探讨隐睾与热休克蛋白和凝集素的出现/消失之间的关系。除了海豚，我们使用实验隐睾小鼠，性腺不活跃的仓鼠睾丸和小鼠睾丸在胎儿和出生后早期阶段（prespermatogenesis）。在实验性隐睾小鼠中，许多变性细胞表现为核质浓缩。TUNEL阳性细胞频繁观察。这些数据提供了至少一些细胞因凋亡而死亡的证据。而hsp70.2在粗线期精母细胞中表达 ...更多信息 和精子细胞。隐睾未检测到反应。在仓鼠睾丸中，暴露于短日照周期后13周，精子发生严重中断。双花扁豆凝集素（DBA）与精原细胞特异性结合。DBA在活动期仅与A型精原细胞结合，而在非活动期与A型、中间型和B型精原细胞结合。在精子发生前，sWGA，VVA和莱亚凝集素的特征结合模式在生殖细胞中观察到。这些凝集素可能与生殖细胞的分化有关。在白海豚中，hsp 86在B型精原细胞、精母细胞和精子细胞中呈强阳性。Hsp 84在B型精原细胞中呈强阳性表达，而在精母细胞和精子细胞中无表达。在里索海豚中，hsp 86在粗线期和细线期前精母细胞中表达。Hsp 84与B型精原细胞反应。这些结果表明，每种蛋白质可能在精子发生的不同过程中发挥作用。少

项目成果

Ito,K., Hayashi,Y.et al.: "Apoptosis-like cell death in experimentally-induced cryptorchidism in adult mouse" Journal of Veterinary Medical Science. 59(5). 353-359 (1997)

Ito,K.、Hayashi,Y.et al.：“成年小鼠实验诱导的隐睾中的细胞凋亡样细胞死亡”《兽医医学科学杂志》。

Suda,A.,Hayashi,T.et al.: "Distribution of lectin binding in spermatogonia of Syrian hamsters in gonadally active and inactive states." Journal of Veterinary Medical Science. 60・2. 189-195 (1998)

Suda, A., Hayashi, T. 等人：“性腺活跃和非活跃状态下叙利亚仓鼠精原细胞中凝集素结合的分布”，兽医医学杂志 60・2（1998 年）。

Suda, A., Hayashi, Y.et al.: "Distribution of lectin binding in spermatogonia of Syrian hamsters in gonadally active and inactive states." Journal of Veterinary Medical Science. 60 (2). 189-195 (1998)

Suda, A.、Hayashi, Y.等人：“性腺活跃和不活跃状态下叙利亚仓鼠精原细胞中凝集素结合的分布。”

Ito, K., Hayashi, Y.et al.: "Apoptosis-like cell death in experimentally-induced cryptorchidism in adult mice." Journal of Veterinary Medical Science. 59 (5). 353-359 (1997)

Ito, K.、Hayashi, Y.等人：“实验诱导的成年小鼠隐睾中的细胞凋亡样细胞死亡。”

Ito, K.,Hayashi,Y.et al.: "Apoptosis-like cell death in experimentally-induced cryptorchidism in adult mice." Journal of Veterinary Medical Science. 59・5. 353-359 (1997)

Ito, K.、Hayashi, Y. 等人：“实验诱导的成年小鼠隐睾中的细胞凋亡样死亡”，《兽医医学杂志》59·5（1997）。