Analysis of inhibitory mechanism of fertilization between different species
不同物种间受精抑制机制分析
基本信息
- 批准号:07456131
- 负责人:
- 金额:$ 5.5万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:1995
- 资助国家:日本
- 起止时间:1995 至 1996
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
First, we tried to establish the in vitro fertilization (IVF) technique of the Japanese field vole. It is improved by sperm concentration (1-2x10^7 cell/ml for preincubation and 1x10^6 cell/ml for insemination) and hypotaurine (0.1-1 mM). Preincubation of spermatozoa with 1 mM hypotaurine pripor to oocytes insemination did not have a significant effect on fertilization rate. At 96 h after insemination, most cultured embryos developed to the 2-cell stage (68-83%), but development to blastocyst was very low (0-3%) for all treatments. Althogh 43 living offspring were produced after transfer of IVF pronuclear embryos to recipients, the delivery rate of recipients was very low (21%). The litter size of pseudopregnat recipients that produced a litter was 4 to 11, and the rate of in vitro development of transferred embryos in the pregnant recipients was 41%. It would appear that hypotaurine affects IVF rather than spermatozoa preincubation and that IVF embryos can develop to normal offspring. Next, we investigated the mechanism of penetration by field vole spermatozoa of mouse and hamster zonae pellucidae. The field vole spermatozoa that can penetrate the zonae pellucidae of the mouse and hamster could not penetrate the zonae of the rat, mastomys and Mongolian gerbil. The vole spermatozoa that bound to the zona oaf field vole oocytes underwent the acrosome reaction before passing through it. In contrast, the vole spermatozoa that bound to the zonae of mouse and hamster oocytes penetrated the zonae without any sign of the acrosome reaction. The presence or absence of proteinase/hyaluronidase inhibitors in the medium did not make any difference to zona penetration by acrosome-intact vole spermatozoa. These observations suggest that field vole spermatozoa can cross mouse and hamster zonae mechanically without assistance from zona-hydrolysing enzymes.
首先,我们试图建立日本田鼠的体外受精(IVF)技术。通过精子浓度(预孵育1-2x10^7个细胞/ml,授精1 x10^6个细胞/ml)和亚牛磺酸(0.1-1 mM)可以提高精子浓度。用1 mM亚牛磺酸预孵育精子,对受精率无显著影响。在授精后96 h,大多数培养的胚胎发育到2-细胞期(68-83%),但发育到囊胚的比例非常低(0-3%)。将体外受精原核胚胎移植给受体后,虽然产生了43个存活后代,但受体的分娩率很低(21%)。假孕受体每窝产4 ~ 11胎,妊娠受体移植胚胎体外发育率为41%。看来亚牛磺酸影响IVF而不是精子预孵育,IVF胚胎可以发育成正常的后代。接下来,我们研究了田鼠精子穿透小鼠和仓鼠透明带的机制。田鼠精子能穿透小鼠和仓鼠的透明带,但不能穿透大鼠、乳鼠和长爪沙鼠的透明带。田鼠精子与野鼠卵母细胞结合后,在穿过卵母细胞前发生顶体反应,而与小鼠和仓鼠卵母细胞结合后,在穿过卵母细胞前没有发生顶体反应。培养基中蛋白酶/透明质酸酶抑制剂的存在或不存在对顶体完整的田鼠精子的精子穿透没有任何影响。这些观察结果表明,田鼠精子可以跨越小鼠和仓鼠透明带机械没有帮助的透明带水解酶。
项目成果
期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Wakayama,Hayashi et al.: "In vitro fertilization/embryo transfer(IVF/ET) of Japanese field vole,Microtus montebelli" Advances in contraceptive delivery systems. (in press). (1996)
Wakayama,Hayashi 等人:“日本田鼠蒙特贝利体外受精/胚胎移植(IVF/ET)”避孕递送系统的进展。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Wakayama,Hayashi et al.: "In vitro fertilization/embryo transfer(IVF/ET)of Japanese field vole,Microtus montebelli" Advances in contraceptive delivery systems. 12(1). 69-77 (1996)
Wakayama,Hayashi 等人:“日本田鼠蒙特贝利体外受精/胚胎移植(IVF/ET)”避孕递送系统的进展。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Wakayama,Hayashi et al.: "In vitro fertilization/embryo transfer (IVF/ET) of Japanese field vole,Microtus montebelli." Advances in contraceptive delivery systems. 12(1). 69-77 (1996)
Wakayama,Hayashi 等人:“日本田鼠 (Microtus montebelli) 的体外受精/胚胎移植 (IVF/ET)”。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Wakayama, T., Suto, J., Matubara, Y., Imamura, K., Fukuta, K., Toyoda, Y., Kurohmaru, M.& Hayashi, Y.: "In vitro fertilization and embryo development of Japanese field voles (Microtus montebelli)" J.Reprod.Fertil.104(1). 63-68 (1995)
Wakayama, T.、Suto, J.、Matubara, Y.、Imamura, K.、Fukuta, K.、Toyoda, Y.、Kurohmaru, M.
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Wakayama, T., Suto, J., Kurohmaru, M.& Hayashi, Y.: "In vitro fertilization/embryo transfer (IVF/ET) of Japanese field vole, Microtus montebelli" Advances in contraceptive delivery systems. 12(1). 69-77 (1996)
和歌山,T.,须藤,J.,黑王丸,M.
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- 影响因子:0
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HAYASHI Yoshihiro其他文献
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自由基共聚的理论意义
- DOI:
10.2477/jccj.2018-0024 - 发表时间:
2018 - 期刊:
- 影响因子:0
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NISHIKUBO Ryo;HAYASHI Yoshihiro;KAWAUCHI Susumu - 通讯作者:
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10.2477/jccj.2018-0025 - 发表时间:
2018 - 期刊:
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HAYASHI Yoshihiro;OHKI Ryuma;HIWAKI Yusuke;KAWAUCHI Susumu - 通讯作者:
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ロジウム触媒を用いたN-アセチルアミノチオフェ ン類とアルキンの脱水素カップリング反応
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2023 - 期刊:
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HAYASHI Yoshihiro;SATOH Tetsuya;MIURA Masahiro;KAWAUCHI Susumu;林幹史朗,臼杵克之助,佐藤哲也 - 通讯作者:
林幹史朗,臼杵克之助,佐藤哲也
HAYASHI Yoshihiro的其他文献
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{{ truncateString('HAYASHI Yoshihiro', 18)}}的其他基金
ヒト肝癌予後推定バイオマーカーの開拓と分子標的薬開発の基礎研究
开发评估人类肝癌预后的生物标志物和开发分子靶向药物的基础研究
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19H00430 - 财政年份:2019
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Phylogenelic and evolutionary examinations on Sciuridae family in the Region of Indochinese Peninsula and Formosa
中南半岛和福尔摩沙地区松鼠科的系统发育和进化研究
- 批准号:
14405030 - 财政年份:2002
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Establishment of testis in vitro assay system for risk assessment of endocrine disrupters
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13460130 - 财政年份:2001
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Analysis of mechanism of changes in function and size of golden hamster testis with special reference to TGF-β signal transduction
特别关注TGF-β信号转导的金黄地鼠睾丸功能和大小变化机制分析
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11460137 - 财政年份:1999
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$ 5.5万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
Cellular and molecular analysis of cryptic testes in dolphins
海豚隐睾的细胞和分子分析
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09460125 - 财政年份:1997
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09556059 - 财政年份:1997
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Epidemiological study of snakebites in Nepal
尼泊尔蛇咬伤流行病学研究
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07041157 - 财政年份:1995
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$ 5.5万 - 项目类别:
Grant-in-Aid for international Scientific Research
Functional participation in endocrine system : activin-follistsatin interaction
内分泌系统的功能参与:激活素-卵泡抑素相互作用
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04454114 - 财政年份:1992
- 资助金额:
$ 5.5万 - 项目类别:
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Establishment of in vitro culture system of seminiferous epitherium and clarifying the mechanism of spermatogenesis
生精上皮体外培养体系的建立及精子发生机制的阐明
- 批准号:
02454098 - 财政年份:1990
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Mechanism for the Formation of Seminiferous Tubules in Mammals
哺乳动物生精小管的形成机制
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63560285 - 财政年份:1988
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$ 5.5万 - 项目类别:
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