Functional participation in endocrine system : activin-follistsatin interaction

内分泌系统的功能参与：激活素-卵泡抑素相互作用

• 批准号: 04454114
• 负责人: HAYASHI Yoshihiro
• 金额: $ 4.42万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454114/
• 关键词: ACTIVIN; FOLLISTATIN; OVARY; TESTIS; PANCREATIC ISLET; 内分泌細胞

The localization ob betaA subunit of inhibin/activin in rat testis was studied by means of immunohistochemistry using antibody raised against synthetic polypeptides. The intense reaction with and-betaA antibody was shown in the cytoplasm but not in the nucleus and the plasma membrane of late pachytene spermatocytes. The reaction was also found in the cytoplasm of spermatids, although the immunostaining pattern was different between the two cells ; the granule-like one in spermatocytes and diffused one in spermatids, respectively. Furthermore, the plasma membrane became positive in spermatids, suggesting that betaA subunit may be produced in spermatocytes phase and secreted from spermatids phase. These results suggest that inhibin or activin may have a significant role in spermatogenesis.Cyclic changes in follistatin localization and the role of primary gonadotropin surges in regulating follistatin expression were studied in rat ovary. Immunoreactive follistatin was detected in the gran … More ulosa cells of secondary to mature follicles by immunohistochemistry using polyclonal antibodies raised against synthetic peptides of human follistatin. Granule-like reactions with Anti-follistatin (123-134) in the granulosa cells of mature follicles drastically decreased their intensity on the evening of proestrus. The blockade of primary gonadotropin surges by injection of pentobarbitone (40 mg/kg) at 1330h on proestrus, resulted in sustaining the follistatin granules in mature follicles at 2300h on proestrus. The sustainment of follistatin was broken down by injection of exogenous LH (20 mug) or FSH (10 mug) to pentobarbitone-treated animals at 1530h on proestrus. These results indicate that expression of follistatin in preovulatory follicles is suppressed by primary gonadotropin surge on proestrus. These drastic changes in follistatin in the preovulatory follicles suggest that follistatin plays a significant role in some physiological phenomena such as cumulus expansion and germinal vesicle break down prior to ovulation.We first detected the mRNA expression of follistatin and three subunits of inhibin/activin in rat pancreatic islets by RT-PCR.Immunohistochemistry using anti-follistatin serum revealed that follistatin was localized only in insulin producing B cells. Although the betaA subunit was detectable in the islets, the immunostainable cell type were completely different with two betaA antisera, i.e.anti-betaA (1-10)-Tyr stained B cells, while anti-betaA (87-99) stained glucagon producing A cells. This inconsistent immunoreactivity was probably related to follistatin binding to subunits of inhibin/activin. This study indicates that follistatin and inhibin/activin in the islet serve as paracrine or autocrine modulators in the endocrine pancreas. Less

用免疫组织化学方法研究了合成多肽在大鼠睾丸中的定位。与抗β-A抗体的强反应只在粗线期晚期精母细胞的细胞质中出现，而在细胞核和质膜中不出现。该反应也被发现在精子细胞的细胞质中，虽然两种细胞之间的免疫染色模式是不同的，颗粒状的一个在精母细胞和扩散的一个在精子细胞，分别。精子细胞质膜呈阳性，提示β A亚单位可能在精母细胞期产生，由精子细胞分泌。这些结果表明，卵泡抑素或激活素可能有显着的作用，在精子发生。卵泡抑素定位的周期性变化和初级促性腺激素激增在调节卵泡抑素表达的作用在大鼠卵巢进行了研究。免疫反应性卵泡抑素检测在卵巢癌 ...更多信息 使用抗人卵泡抑素合成肽的多克隆抗体，通过免疫组织化学法观察次级成熟卵泡的卵泡膜细胞。抗卵泡抑素（123-134）在成熟卵泡颗粒细胞中的颗粒样反应在发情前期的晚上显著降低。在发情前期1330 h注射戊巴比妥钠（40 mg/kg）阻断初级促性腺激素峰，可使卵泡中卵泡抑素颗粒维持在发情前期2300 h。在发情前期1530 h，通过向戊巴比妥处理的动物注射外源性LH（20 μ g）或FSH（10 μ g），破坏卵泡抑素的维持。这些结果表明卵泡抑素在排卵前卵泡中的表达受到发情前期初级促性腺激素峰的抑制。本研究首次采用RT-PCR方法检测了卵泡抑素和卵泡素/激活素三个亚基在大鼠胰岛中的mRNA表达，并采用抗卵泡抑素血清进行免疫组化，结果显示卵泡抑素仅定位于胰岛素分泌B细胞。尽管胰岛中可检测到β A亚单位，但两种β A抗血清的免疫抑制细胞类型完全不同，即抗β A（1-10）-Tyr染色的B细胞，而抗β A（87-99）染色的胰高血糖素产生A细胞。这种不一致的免疫反应性可能与卵泡抑素结合到亚单位的follistatin/activin。这项研究表明，胰岛中的卵泡抑制素和抑制素/激活素在内分泌胰腺中充当旁分泌或自分泌调节剂。少

项目成果

Ogawa K,Abe K,Kurosawa N,Kurohmaru M,Sugino H,Takahashi M and Hayashi Y: "Expression of α,βA and βB subunits of inhibin or activin and follistatin in rat pancreatic islets." FEBS lett. 319. 217-220 (1993)

Okawa K、Abe K、Kurosawa N、Kurohmaru M、Sugino H、Takahashi M 和 Hayashi Y：“大鼠胰岛中抑制素或激活素和卵泡抑素的 α、βA 和 βB 亚基的表达。 FEBS lett。” (1993)

Ogawa K,Kurohmaru M,Sugino H and Hayashi Y: "Changes in the immunoreactivity of follistatin within preovulatory follicles after gonadotrophin surge in rat" J Reprod Fert. (投稿中).

Okawa K、Kurohmaru M、Sugino H 和 Hayashi Y：“大鼠促性腺激素激增后排卵前卵泡内卵泡抑素免疫反应性的变化”J Reprod Fert。

Ogawa K,Kurohmaru M,Shiota K,Takahashi M and Hayashi Y: "Immunoreactive βA subunit of inhibin/activin is present in cytoplasm of rat spermatogenic cells." J Reprod Dev. 38. 5-9 (1992)

Okawa K、Kurohmaru M、Shiota K、Takahashi M 和 Hayashi Y：“抑制素/激活素的免疫反应性 βA 亚基存在于大鼠生精细胞的细胞质中。”38. 5-9 (1992)

Ogawa K,Kurohmaru M,Sugino H and Hayashi Y: "Immunoreactive follistatin in rat spermatogenic cells" (投稿準備中).

Okawa K、Kurohmaru M、Sugino H 和 Hayashi Y：“大鼠生精细胞中的免疫反应性卵泡抑素”（准备提交）。

Ogawa K, Kurohmaru M, Shiota K, Tahahashi M and Hayashi Y: "Immunoreactive betaA subunit of inhibin/activin is present in cytoplasm of rat spermatogenic cells" J Reprod Dev. 38. 5-9 (1992)

Okawa K、Kurohmaru M、Shiota K、Tahahashi M 和 Hayashi Y：“抑制素/激活素的免疫反应性 betaA 亚基存在于大鼠生精细胞的细胞质中”J Reprod Dev。