Task-dependent Orchestration of Insect Olfactory Sensory Neuron Performance

昆虫嗅觉感觉神经元性能的任务依赖性编排

• 批准号: 430156010
• 负责人: Professor Dr. Robert J. Kittel
• 金额: --
• 依托单位: Max-Planck-Institut für Chemische Ökologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/430156010?language=en
• 关键词: Task; dependent; Orchestration; Insect; Olfactory

Sensory systems have to provide an appropriate representation of the environment. What is appropriate depends on the meaning of a signal for the organism. In the proposed project we investigate the tuning of insect odorant receptors. Olfactory sensory neurons expressing the receptor Or56a which detects the alarm odor geosmin, a product of toxin producing microbes, are permanently tuned to highest sensitivity. By contrast, cells expressing the food odor-detecting Or22a are set to moderate sensitivity under resting conditions but can be sensitized on demand. The aim of this project is to investigate how these different properties in sensory neuron performance are realized and manifested at various levels, such as odorant receptor expression density, downstream signal processing and amplification within the compartments of the receptor neuron. The comparative approach will target the olfactory signaling chain of Or22a and Or56a expressing olfactory sensory neurons at the various levels. For this sake we will apply super resolution fluorescence microscopy as well as the electrophysiological patch clamp technique.

感官系统必须提供对环境的适当表征。什么是适当的取决于信号对生物体的意义。在拟议的项目中，我们调查昆虫气味受体的调谐。嗅觉感觉神经元表达受体Or56a，其检测警报气味土臭素，一种产毒素微生物的产物，被永久地调整到最高灵敏度。相比之下，表达食物气味检测Or22a的细胞在静息条件下被设定为中等灵敏度，但可以根据需要被敏化。该项目的目的是研究感觉神经元性能中的这些不同特性是如何在不同水平上实现和表现的，例如气味受体表达密度，下游信号处理和受体神经元隔室内的放大。比较方法将靶向在各个水平上表达嗅觉感觉神经元的Or22a和Or56a的嗅觉信号传导链。为此，我们将应用超分辨率荧光显微镜以及电生理膜片钳技术。