Physiology and plasticity of the active zone in vivo

体内活性区的生理学和可塑性

• 批准号: 115506279
• 负责人: Professor Dr. Robert J. Kittel
• 金额: --
• 依托单位: Arbeitsgruppe Tierphysiologie
• 依托单位国家: 德国
• 项目类别: Independent Junior Research Groups
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/115506279?language=en
• 关键词: Physiology; plasticity; active; zone; vivo

Synapses are specialised intercellular contact sites, which serve as the communication link between neurons and their partner cells. At chemical synapses, calcium-ion (Ca2+) influx triggers the fusion of transmitter-laden vesicles with the presynaptic membrane at a specific sub-cellular region termed the active zone. Transmitter substances released by this process then diffuse across the synaptic cleft and are sensed by postsynaptic receptors to convey signal transduction. A hallmark of synaptic transmission is its plasticity, which enables synapses to regulate complex brain processes by filtering, modifying, or integrating information. The details of active zone physiology and how its modulation contributes to synaptic plasticity are, however, barely understood. By combining genetics with highresolution opto- and electrophysiological methods in Drosophila melanogaster, this project will test the hypothesis that active zone physiology is modified during activity-induced plasticity in vivo. To this end, molecular dynamics will be followed at single synapse resolution and neurotransmission will be both evoked and measured in an intact, alive, and genetically most amenable organism.

突触是专门的细胞间接触点，是神经元与其伴侣细胞之间的通讯纽带。在化学突触中，钙离子（Ca2+）内流触发递质囊泡与突触前膜在特定亚细胞区域的融合，称为活性区。这一过程释放的递质物质随后扩散穿过突触间隙，并被突触后受体感知以传递信号转导。突触传递的一个特点是它的可塑性，这使得突触能够通过过滤、修改或整合信息来调节复杂的大脑过程。然而，活跃区生理学的细节以及它的调节如何促进突触可塑性，人们几乎不了解。本项目将结合遗传学和高分辨率的光和电生理方法，在黑腹果蝇体内验证活动区生理在活动诱导的可塑性过程中被改变的假设。为此，分子动力学将在单个突触分辨率下进行，神经传递将在一个完整的、活的、遗传上最易适应的生物体中被唤起和测量。

项目成果

Naked Dense Bodies Provoke Depression

• DOI: 10.1523/jneurosci.2495-10.2010
• 发表时间: 2010-10-27
• 期刊: JOURNAL OF NEUROSCIENCE
• 影响因子: 5.3
• 作者: Hallermann, Stefan;Kittel, Robert J.;Heckmann, Manfred
• 通讯作者: Heckmann, Manfred

Optogenetics in Drosophila Neuroscience.

• DOI: 10.1007/978-1-4939-3512-3_11
• 发表时间: 2016
• 期刊: Methods in molecular biology
• 作者: T. Riemensperger;R. Kittel;A. Fiala

Super-resolution microscopy of the synaptic active zone

• DOI: 10.3389/fncel.2015.00007
• 发表时间: 2015-01-30
• 期刊: FRONTIERS IN CELLULAR NEUROSCIENCE
• 影响因子: 5.3
• 作者: Ehmann, Nadine;Sauer, Markus;Kittel, Robert J.
• 通讯作者: Kittel, Robert J.

Quantitatives imaging durch molekulare Auflösung

通过分子分辨率进行定量成像

• DOI: 10.1007/s12268-014-0495-1
• 发表时间: 2014
• 期刊: BIOspektrum
• 作者: van de Linde S;Ehmann N;Kittel RJ;Sauer M
• 通讯作者: Sauer M

The adhesion GPCR latrophilin/CIRL shapes mechanosensation.

• DOI: 10.1016/j.celrep.2015.04.008
• 发表时间: 2015-05
• 期刊: Cell reports
• 影响因子: 8.8
• 作者: Nicole Scholz;J. Gehring;Chonglin Guan;Dmitrij Ljaschenko;Robin Fischer;Vetrivel Lakshmanan;R. Kittel;T. Langenhan