Pathophysiology of MNMS due to acute arterial occlusion and development of a local perfusion

急性动脉闭塞和局部灌注引起的 MNMS 的病理生理学

基本信息

  • 批准号:
    02454302
  • 负责人:
  • 金额:
    $ 4.16万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
  • 财政年份:
    1990
  • 资助国家:
    日本
  • 起止时间:
    1990 至 1992
  • 项目状态:
    已结题

项目摘要

Twenty-five dogs were used to investigate myonephropathic metabolic syndrome(MNMS). After 24-hour ischemia by ligation of abdominal aorta, the ligation was released and blood sample was taken from the vena cava before reperfusion. Just after reperfusion, 30 and 60 minutes after reperfusion. The levels of CPK, 6-keto PGF_1 alpha, 11-dehydrothromboxane B_2 were elevated during ischemia-reperfusion. White blood cell(WBC) increased just after reperfusion and decreased at 30 minutes after reperfusion. It is considered that this is because WBC migrate into the injured tissue.As the next step, we investigated the time course and the source of the free radicals generated in the rat isolated gracilis muscle after 2hr of ischemia followed by 1hr of reperfusion. Free radicals were measured by electron spin resonance(ESR). The intensity pattern of free radicals showed two peaks, the first peak was at 3min after reperfusion (I/R3) and the second peak lasted from 45min to 60min after reperfusion. The ESR intensities atI/R3 and I/R60 were inhibited in both X0 inhibitor(OPF-001 Ootsuka, Japan) group and neutropenia rat group. Myeloperoxidase(MPO) activity was also measured at I/R3,20,60. The increase at I/R3 and I/R60 in MPO activity showed the same pattern with the two peaks of free radical intensities. Therefore the first ESR peak of free radicals may be derived from the endothelial cells and the second ESR peak from the neutrophils. It is supposed, however, that an interaction between the endothelial cells and neutrophils starts at early phase(I/R3) of reperfusion.In conclusion, WBC play an very important role in ischemia-reperfusion in skeletal muscle and the local perfusion device with a filter for WBC may be useful to prevent MNMS.
对25只犬进行了肌肾病代谢综合征(MNMS)的研究。腹主动脉结扎缺血24小时后,松解结扎,于再灌流前从下腔静脉取血。再灌流即刻、再灌流后30分钟和60分钟。CPK、6-keto-PGF_1α、11-脱氢血栓素B_2在缺血再灌流过程中升高。白细胞(WBC)在再灌流后即刻升高,再灌流后30min下降。下一步,我们研究了大鼠离体股薄肌缺血2小时再灌流1小时后产生的自由基的时间进程和来源。用电子自旋共振(ESR)法测定自由基。自由基强度呈现两个高峰,第一个峰值出现在再灌流后3min(I/R3),第二个峰值持续在再灌流后45min~60min。X0抑制剂(OPF-001 Ootsuka,日本)组和中性粒细胞减少大鼠组的ESR强度ATI/R3和I/R60均受到抑制。在I/R3、20、60时测定髓过氧化物酶(MPO)活性。MPO活性在I/R3和I/R60时的升高与自由基强度的两个峰值呈现相同的规律。因此,自由基的第一个ESR峰可能来自内皮细胞,第二个ESR峰可能来自中性粒细胞。然而,据推测,内皮细胞和中性粒细胞之间的相互作用始于再灌流的早期阶段(I/R3)。综上所述,WBC在骨骼肌缺血再灌注中起着非常重要的作用,具有WBC过滤器的局部灌流装置可能有助于预防MNMS。

项目成果

期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
矢野 義明: "骨格筋の虚血再潅流モデルにおけるフリーラジカルの経時的変化とその発生源についての実験的考察" 日本血管外科学会雑誌. 1. 41-47 (1992)
Yoshiaki Yano:“骨骼肌缺血再灌注模型中自由基的时间变化及其来源的实验研究”日本血管外科学会杂志 1. 41-47 (1992)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Yoshiaki Yano: "Time Course and the Source of Free Radicals after Ischemia Reperfusion in Skeletal Muscle" The Japanese Journal of Vascular Surgery. 1. 41-47 (1992)
Yoshiaki Yano:“骨骼肌缺血再灌注后自由基的时间进程和来源”日本血管外科杂志。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

KANEKO Hiroshi其他文献

KANEKO Hiroshi的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('KANEKO Hiroshi', 18)}}的其他基金

Elucidation of the diversity of target genes in GATA1
阐明GATA1中靶基因的多样性
  • 批准号:
    24890015
  • 财政年份:
    2012
  • 资助金额:
    $ 4.16万
  • 项目类别:
    Grant-in-Aid for Research Activity Start-up
Drug development for osteoarthritis(OA) by drug screening for transcriptional activation of SOX9
通过 SOX9 转录激活药物筛选来开发骨关节炎 (OA) 药物
  • 批准号:
    22659270
  • 财政年份:
    2010
  • 资助金额:
    $ 4.16万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Reconstructionof a semanticsbased on the notion of proof
基于证明概念的语义重构
  • 批准号:
    22520032
  • 财政年份:
    2010
  • 资助金额:
    $ 4.16万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Low Temperature x-ray diffraction study on phase transition
相变的低温X射线衍射研究
  • 批准号:
    19540363
  • 财政年份:
    2007
  • 资助金额:
    $ 4.16万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Philosophical and Historical research into varieties of Constructivism in Logic and Mathematics
对逻辑和数学中各种建构主义的哲学和历史研究
  • 批准号:
    18520025
  • 财政年份:
    2006
  • 资助金额:
    $ 4.16万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Analysis on Pathophysiology of Functional Gastrointestinal Disorders (FGIDs) and Establishment of Comprehensive Evaluation Methods and Treatment of FGIDs
功能性胃肠病(FGIDs)的病理生理学分析及FGIDs综合评估方法和治疗方法的建立
  • 批准号:
    15590608
  • 财政年份:
    2003
  • 资助金额:
    $ 4.16万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Research on Brouwer's Philosophy and the notion of continuum in Intuitionism
布劳威尔哲学与直觉主义连续统概念研究
  • 批准号:
    15520026
  • 财政年份:
    2003
  • 资助金额:
    $ 4.16万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Historical and Logical Investigation into Hilbert Program as a philosophy of mathematics
作为数学哲学的希尔伯特纲领的历史和逻辑研究
  • 批准号:
    13610014
  • 财政年份:
    2001
  • 资助金额:
    $ 4.16万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Investigation into relations between realism and constructivism in the philosophy of mathematics
数学哲学中实在论与建构主义关系探讨
  • 批准号:
    06610013
  • 财政年份:
    1994
  • 资助金额:
    $ 4.16万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

相似海外基金

A novel treatment for REBOA complications: Hydrogen gas inhalation therapy to alleviate oxidative stress due to ischemia-reperfusion injury
REBOA并发症的新型治疗方法:氢气吸入疗法减轻缺血再灌注损伤引起的氧化应激
  • 批准号:
    23K21458
  • 财政年份:
    2024
  • 资助金额:
    $ 4.16万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development ofsynthetic heparin to protect liver graft from ischemia reperfusion injury duringtransplantation
开发合成肝素以保护移植肝免受移植过程中的缺血再灌注损伤
  • 批准号:
    10759102
  • 财政年份:
    2023
  • 资助金额:
    $ 4.16万
  • 项目类别:
Nck1 in Ischemia Reperfusion Injury
Nck1在缺血再灌注损伤中的作用
  • 批准号:
    10715406
  • 财政年份:
    2023
  • 资助金额:
    $ 4.16万
  • 项目类别:
The Role of Neutrophils in Ischemia/Reperfusion Injury following Acute Stroke
中性粒细胞在急性中风后缺血/再灌注损伤中的作用
  • 批准号:
    10606952
  • 财政年份:
    2023
  • 资助金额:
    $ 4.16万
  • 项目类别:
Zinc Protection Against Ischemia-Reperfusion Injury in Heart
锌可预防心脏缺血再灌注损伤
  • 批准号:
    10652915
  • 财政年份:
    2023
  • 资助金额:
    $ 4.16万
  • 项目类别:
Ischemia/Reperfusion injury and Myocardial edema
缺血/再灌注损伤和心肌水肿
  • 批准号:
    10718260
  • 财政年份:
    2023
  • 资助金额:
    $ 4.16万
  • 项目类别:
Role of Gasdermin D/E in intestinal ischemia-reperfusion injury
Gasdermin D/E 在肠缺血再灌注损伤中的作用
  • 批准号:
    23K15529
  • 财政年份:
    2023
  • 资助金额:
    $ 4.16万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
Monocytic-MDSCs as resolution mediators of post-transplant lung ischemia-reperfusion injury
单核细胞-MDSC作为移植后肺缺血再灌注损伤的解决介质
  • 批准号:
    10677290
  • 财政年份:
    2023
  • 资助金额:
    $ 4.16万
  • 项目类别:
Role of Lipid Metabolism in Hepatic Ischemia Reperfusion Injury in Steatotic Livers
脂质代谢在脂肪肝缺血再灌注损伤中的作用
  • 批准号:
    10664736
  • 财政年份:
    2023
  • 资助金额:
    $ 4.16万
  • 项目类别:
ETS2-dependent control in cardiomyocyte ischemia/reperfusion injury
ETS2 依赖性控制心肌细胞缺血/再灌注损伤
  • 批准号:
    10501545
  • 财政年份:
    2022
  • 资助金额:
    $ 4.16万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了