Elucidation of the diversity of target genes in GATA1

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Transcription factor GATA1 has two distinct transactivation domains (N-TAD and C-TAD). It remains to be uncovered what these multiple TADs systems confers on GATA1 function in vivo. In this study, I revealed that GATA1 target genes were classified into three groups with their dependency to TAD. In addition to this GST-pull down assay showed that GATA1 and Rb interaction was dependent to N-TAD. Because, GATA1 mutations which causes the deletion of N-TAD are found in Down's related acute megakaryoblastic leukemia(DS-AMkL) and transient myeoproliferative disorder(TMD), this result suggests that GATA1 transcriptional regulatory mechanism based on multiple TAD system is involvement to the pathology of DS-AMkL and TMD.