Studies on diagnostic methods for measurement of periodontal disease activity

本研究旨在探讨牙周病活动性的诊断方法。实验一，对39例牙周炎患者的17个牙周健康部位和53个牙周病变部位的龈下菌斑和龈沟液进行了检测。龈下菌斑细菌绝对计数。牙龈卟啉单胞菌（Pg）、中间普雷沃菌（Pi）、前列腺素E_2（PGE_2）和白细胞介素-1 α（IL-1 α）在龈沟液中所占比例在患病部位明显增高。实验二对10例未经治疗的成人牙周炎患者和7例经牙周治疗后仍保持牙周炎状态的患者的活动性和非活动性龈下菌斑和龈沟液进行了检测。活动性病变部位定义为在42天内显示结缔附着丧失超过2 mm的囊袋 ...更多信息 监测期。非活动性病变部位的袋深度与活动性部位相当，但结缔组织附着水平在监测期间没有变化。结果表明：牙周炎患者活动区龈沟液中PGE_2含量和龈下菌斑细菌绝对计数均显著高于非活动区，而活动区龈沟液中PGE_2含量和龈下菌斑细菌绝对计数均显著高于非活动区。GCF中的IL-1 α、IL-1 β、活性胶原酶和活动杆比例在活动部位升高。在牙周炎患者的维持治疗，细菌的绝对计数显着较高的活动部位，GCF中的PGE_2升高更经常在这样的网站。线性判别分析的数据被证明是有用的检测活动部位在未经治疗的牙周炎，因为单独的每一个测试不能区分它们。PGE_2、IL-1 α、IL-1 β、LPS和胶原酶联合检测对活动性病变的敏感性和特异性分别为62%和100%。相反，细菌绝对计数本身可用于检测牙周炎患者维持治疗中的活动部位。其敏感性和特异性均为82%。因此，线性判别分析可作为牙周病活动性的诊断方法之一。少

This study was designed to investigate diagnostic methods for measurement of periodontal disease activity. In the first experiment, subgingival plaque and gingival crevicular fluid (GCF) were taken from 17 periodontally health sites and 53 diseased sites in 39 subjects with untreated periodontitis. Absolute counts of subgingival plaque bacteria. the proportion of Spirochetes, Motile rods, Porphyromonas gingivalis (Pg) and Prevotella intermedia (Pi), prostaglandin E_2 (PGE_2) and interleukin-1alpha (IL-1alpha) in GCF were significantly higher in diseased sites. The results suggested that these tests may aid in diagnosis of periodontal disease.In the second experiment, subgingival plaque and GCF were taken from active and inactive lesions in 10 subjects with untreated adult periodontitis and also from 7 subjects in whom periodontitis was maintained after periodontal treatment. Active diseased sites were defined as pockets showing more than 2mm loss of connective attachment during 42-day … More monitoring period. Inactive diseased sites had a pocket depth comparable to the active sites, but connective attachment level did not change during the monitoring period. Subgingival plaque bacteria, and PGE_2, IL-1alpha, IL-1beta, collagenase activity and lipopolysaccharide(LPS)in GCF we're compared in active and inactive sites.In untreated periodontitis patients, PGE_2 in GCF and absolute counts of subgingival plaque bacteria were significantly higher in active sites. IL-1alpha, IL-1beta, active collagenase in GCF and the proportion of motile rods were elevated more often in active sites. In periodontitis patients on maintenance treatment, absolute counts of bacteria were significantly higher at active sites, and PGE_2 in GCF was elevated more often at such sites.Linear discriminant analysis of data was shown to be useful for detecting active sites in untreated periodontitis, because each test alone could not distinguish them. The combination of PGE_2, IL-1alpha, IL-1beta, LPS and collagenase determinations, was most useful for detecting active sites, and the sensitivity and specificity for detection of active lesions was 62% and 100%, respectivity. In contrast, the absolute counts of bacteria could be used by itself for detection of active sites in the periodontitis patients on maintenance treatment. Its sensitivity and specificity were both 82%. In conclusion, the linear discriminant analysis could be one of the useful diagnostic methods for measurement of periodontal disease activity. Less