Development of control methods based on genetic engineering and scientific diagnosis against refractory periapical periodontitis-associated biofilm

我们将生物膜形成细菌集中在感染的根管内和根尖孔外，旨在开发难治性根尖周炎的微生物诊断方法。另一个目的是利用生物膜相关基因或与群体感应抑制剂和抗微生物剂相关的药物来开发具有抑制生物膜生长和抑制预先存在的生物膜能力的抗生物膜药物。特定的细菌种类经常与根外生物膜的形成相关，并且它们与一些临床症状有关。此外，我们还发现了生物膜相关基因，以及显示抗生物膜作用的常规抗生素或群体感应相关材料。我们为化学和遗传生物膜控制方法的临床应用奠定了基础。我对这些研究结果感到自豪，这些结果为建立难治性根尖周炎的新治疗策略提供了有用的建议。

We focused the biofilm-forming bacteria in the infected root canal and out of the apical foramen, and aimed at the development of the microbiological diagnostic method of refractory periapical periodontitis. The other purpose was the development of the anti-biofilm drugs possessed inhibition of biofilm growth and suppression of preexisting biofilm ability using the biofilm-related gene or the drug associated with the quorum sensing inhibitor and anti-microbial agent.The specific bacterial species were frequently associated with the extraradicular biofilm formation, and they relate to some clinical symptoms. In addition, we have discovered the biofilm-associated gene, and the conventional antibiotics or the quorum sensing related material indicating the anti-biofilm action. And we built up the basis for the clinical application of the chemical and genetic biofilm control methods.I take pride in these results of the research giving the suggestion that is useful for the establishment of a new treatment strategy of refractory periapical periodontitis.