Studies on analysis of biofilm formation in dental plaque and on control methods for the biofilm formation.

牙菌斑生物膜形成分析及生物膜形成控制方法的研究。

• 批准号: 09470422
• 负责人: EBISU Shigeyuki
• 金额: $ 7.68万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470422/
• 关键词: Dental plaque; Biofilm; Biofilm forming bacteria; Scanning immunoelectron microscopy method; Glycocalyx; Modified Robbin's device; Modified Robbin's Devic

Teeth with severe advanced adult periodontitis were extracted and investigated whether a bacterial biofilm concerned with bacterial colonization and growth of dental plaque by scanning microscopy. The results of the study revealed that bacterial biofilms are related to colonization and growth of supra and subgingival plaque. By scanning immunoelectron microscopic techniques using both secondary and back-scattered imaging with rabbit antibodies specific for each bacteria, Actinomyces viscosus, Streptococcus mutans and Eubacterium alactolyticum were detected near the glycocalyx structures in the supragingival plaque. In the plaque-free zone, Porphyromonas gingivalis and A.viscosus were covered with an amorphous film-like structure. P.gin givalis were found in 8/13 samples examined, and film-like structures coated several positive cells. A.viscosus were found in 6/11 samples examined, and tended to overlay the amorphous capsula and aggregated. These findings indicated that both P.gingival … More is and A.viscosus were possibly related to biofilm formation in the both supragingival and subgingival plaque. P.gingivalis possibly formed biofilm and resisted host response at the advancing front of subgingival plaque.We developed experimental in vitro model of biofilm formation by A.viscosus. The model used the modified Robbin's device, which provided continuous source of A.viscosus grown in a Brain-Heart Infusion broth (from 3 to 28 days). Formation of bacterial biofilm were observed by scanning electron microscopy during a 28-day experimental period. By day 3, attached A.viscosus were seen but glycocalyx-like structure was not observed. After day 7, A.viscosus grew and embedded in extracellular glycocalyx-like substances. We suggested that the- experimental model developed in the present study was useful for investigating the biofilm formation of plaque bacteria.Caries and periodontal disease are infectious disease caused by plaque bacteria, and it is difficult to throw light on the whole mechanism of biofilm formation in the dental plaque. However, based on the present results, we evaluated several methods of plaque control used, and presented the current concept for the rational plaque control. Less

采用扫描显微镜观察重度牙周炎患者的牙菌斑中细菌生物膜的形成情况。研究结果表明，细菌生物膜与龈上和龈下菌斑的定植和生长有关。通过扫描免疫电子显微镜技术，使用二次和背散射成像与兔抗体特异性的每种细菌，粘性放线菌，变形链球菌和真杆菌alactolyticum检测附近的糖萼结构的龈上菌斑。在无菌斑区，牙龈卟啉单胞菌和粘性放线菌被无定形膜状结构覆盖。在8/13份检查的样品中发现了Gingivalis，并且膜状结构包被若干阳性细胞。在6/11个检查的样品中发现了粘性放线菌，并且倾向于覆盖无定形荚膜并聚集。这些结果表明，牙龈卟啉单胞菌 ...更多信息 粘放线菌和放线菌可能与龈上菌斑和龈下菌斑中生物膜的形成有关。牙龈卟啉单胞菌可能在龈下菌斑的前沿形成生物膜并抵抗宿主反应。该模型使用改良的Robbin装置，其提供在脑心浸液肉汤中生长的粘性放线菌的连续来源（3至28天）。在28天的实验期间，通过扫描电子显微镜观察细菌生物膜的形成。到第3天，观察到附着的粘性放线菌，但未观察到糖萼样结构。第7天后，粘性放线菌生长并包埋在细胞外糖萼样物质中。龋病和牙周病是由菌斑细菌引起的感染性疾病，目前尚难以阐明菌斑生物膜形成的全部机制。然而，根据目前的结果，我们评估了几种方法的菌斑控制，并提出了目前的概念，合理的菌斑控制。少

项目成果

恵比須繁之ら: "現代臨床におけるプラーク・コントロールの考え方(II)機械的および化学的プラーク・コントロール法の意義と効用" ザ・クインテッセンス. 16・12. 2740-2746 (1997)

Shigeyuki Ebisu 等人：“现代临床实践中斑块控制的思考（II）机械和化学斑块控制方法的意义和有效性”The Quintessence 16・12（1997）。

恵比須繁之ら: "現代臨床におけるプラーク・コントロールの考え方(I) 細菌バイオフィルムとしてのデンタル・プラーク" ザ・クインテッセンス. 16・11. 2629-2640 (1997)

Shigeyuki Ebisu 等人：“现代临床实践中牙菌斑控制的概念（I）牙菌斑作为细菌生物膜”16・11 2629-2640（1997）。

Ebisu S.et al: "Current concept of plaque control (I) Bacterial biofilm and dental plque." The quintessence. 16(11). 2629-2640 (1997)

Ebisu S.et al：“当前牙菌斑控制的概念 (I) 细菌生物膜和牙菌斑。”

Noiri Y.et al: "Identification of the biofilm-associated Porphyromonas gingivalis, Campylobacter rectus and Actinomyces viscosus in the"plaque-free zone"" J Periodont Res.(In Press). (1999)

Noiri Y.等人：““无牙菌斑区”中与生物膜相关的牙龈卟啉单胞菌、直肠弯曲杆菌和粘性放线菌的鉴定”《牙周病研究杂志》（正在出版）。

Noiri Y.et al: "Participation of bacterial biofilms in the growth/colonization of dental plaque-bacteria." J Soc Bact Adhe.12(In Press). (1999)

Noiri Y.等人：“细菌生物膜参与牙菌斑细菌的生长/定植。”