Studies on analysis of biofilm formation in dental plaque and on control methods for the biofilm formation.

采用扫描显微镜观察重度晚期成人牙周炎患者牙体内细菌生物膜是否与菌斑的定植和生长有关。研究结果表明，细菌生物膜与牙周上、下菌斑的定植和生长有关。用兔抗菌抗体二次和背向散射成像的扫描免疫电子显微镜技术，在牙周上菌斑的糖萼结构附近检测到粘性放线菌、变形链球菌和溶乳优杆菌。在无菌斑区域，牙龈卟啉单胞菌和粘性卟啉单胞菌被无定形的膜状结构覆盖。13份标本中有8份检出金黄色葡萄球菌，并有多个阳性细胞被膜状结构包裹。11份标本中有6份检出粘性支原体，且有覆盖无定形包膜和聚集的倾向。这些发现表明，牙龈假单胞菌…更多的IS和粘性弧菌可能与龈上菌斑和龈下菌斑中生物膜的形成有关。牙龈假单胞菌可能在龈下菌斑前缘形成生物膜并抵抗宿主反应。该模型使用了改进的Robbin的设备，该设备提供了在脑心输液肉汤中生长的粘性支原体的连续来源(从3天到28天)。在28天的实验期内，用扫描电子显微镜观察细菌生物膜的形成。到第3天，粘性支原体已附着，但未观察到糖萼样结构。培养7d后，粘性支原体生长并包埋在胞外糖萼样物质中。我们认为本实验模型可用于菌斑细菌生物膜形成的研究。龋病和牙周病是由菌斑细菌引起的感染性疾病，很难揭示牙菌斑中生物膜形成的全部机制。然而，基于目前的结果，我们评估了所使用的几种斑块控制方法，并提出了合理的斑块控制的当前概念。较少

Teeth with severe advanced adult periodontitis were extracted and investigated whether a bacterial biofilm concerned with bacterial colonization and growth of dental plaque by scanning microscopy. The results of the study revealed that bacterial biofilms are related to colonization and growth of supra and subgingival plaque. By scanning immunoelectron microscopic techniques using both secondary and back-scattered imaging with rabbit antibodies specific for each bacteria, Actinomyces viscosus, Streptococcus mutans and Eubacterium alactolyticum were detected near the glycocalyx structures in the supragingival plaque. In the plaque-free zone, Porphyromonas gingivalis and A.viscosus were covered with an amorphous film-like structure. P.gin givalis were found in 8/13 samples examined, and film-like structures coated several positive cells. A.viscosus were found in 6/11 samples examined, and tended to overlay the amorphous capsula and aggregated. These findings indicated that both P.gingival … More is and A.viscosus were possibly related to biofilm formation in the both supragingival and subgingival plaque. P.gingivalis possibly formed biofilm and resisted host response at the advancing front of subgingival plaque.We developed experimental in vitro model of biofilm formation by A.viscosus. The model used the modified Robbin's device, which provided continuous source of A.viscosus grown in a Brain-Heart Infusion broth (from 3 to 28 days). Formation of bacterial biofilm were observed by scanning electron microscopy during a 28-day experimental period. By day 3, attached A.viscosus were seen but glycocalyx-like structure was not observed. After day 7, A.viscosus grew and embedded in extracellular glycocalyx-like substances. We suggested that the- experimental model developed in the present study was useful for investigating the biofilm formation of plaque bacteria.Caries and periodontal disease are infectious disease caused by plaque bacteria, and it is difficult to throw light on the whole mechanism of biofilm formation in the dental plaque. However, based on the present results, we evaluated several methods of plaque control used, and presented the current concept for the rational plaque control. Less