Localization of periodontal disease-associated bacteria in human periodontal pockets and its relationship to tissue destruction

牙周病相关细菌在人牙周袋中的定位及其与组织破坏的关系

• 批准号: 04454472
• 负责人: EBISU Shigeyuki
• 金额: $ 4.35万
• 依托单位: Tokushima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454472/
• 关键词: Periodontal disease-associated bacteria; Plaque bacteria; Human periodontal pocket; Localization; Immunohistological observation; Bacterial lectin; B cell mitogen; 歯周ポケット内細菌; 付着因子; 免疫組織学; 微細形態学

This study was designed to observe the localization and distribution of certain plaque bacteria including periodontal disease-associated bacteria in human periodontal pocket. After providing informed consent, teeth and their surrounding periodontal tissue with advanced adult periodontitis were obtained carefully attempting not to change the structure of the periodontal pockets. They were processed into serial sections, and those were stained with Brown-Brenn modified Gram stain and LASB method with specific rabbit antiboeies against fifteen plaque bacterial species including periodontal disease-associated bacteria. The localization and distribution of the bacterial species in human periodontal pockets were observed under a light microscope. A small aggregates of Porphyromonas gingivalis were distributed spattering throughout the periodontal pocket. Eikenella corrodens, shich formed small clumps, was located mainly close to root surface in the middle and deep pocket zones. Fusobacterium … More nucleatum also consisted small clumps in the middle and deep pocket zones, but its localization was in the unattached plaque area. On the other hand, Camphylobacter rectus generaly formed large clumps in the middle and deep pocket zones, and located closely on both root surface and epithelial surface in periodontal pockets. Treponema denticola was observed over the unattached plaque area located mainly in the middle zone of pockets.previously, we found that E.corrodens 1073 had a cell-associated lectin-like substans (EcLS) and it functioned in the adherence of this periodontal disease-associated bacteria to various cell surface. The immunoelectron microscopic study using monoclonal antibody against EcLS revealed that EcLS was localized in the outer space of outer membrane, not in cell surface appendages such as fimbriae where many bacteria possessed adhesin. This EcLS was found to have potent mitogenic activity when cultured with B lymphocytes from BALB/c mice. EcLS stimulated murine B lymphocytes not only to proliferate, but also to differentiate into antibody-secreting cells. These findings suggest that EcLS can stimulate host cells, and does not just mediate adherence between the host cell and E.corrodens. Less

本研究旨在观察包括牙周病相关菌在内的菌斑细菌在人牙周袋中的定位和分布。在提供知情同意书后，小心地获得患有晚期成人牙周炎的牙齿及其周围牙周组织，试图不改变牙周袋的结构。采用Brown-Brenn改良革兰氏染色法和LASB法对牙周病相关菌等15种菌斑细菌进行染色。光镜下观察牙周袋内细菌的分布和定位。牙龈卟啉单胞菌的小聚集体散布在整个牙周袋中。腐蚀艾肯氏菌（Eikenella corrodens）主要分布在中、深囊区靠近根面的位置，形成小丛。梭杆菌 ...更多信息 核质在中、深囊区也有小团块，但主要分布在未附着斑块区。直弯曲杆菌多在牙周袋中、深囊区形成大团块，在牙周袋中紧密分布于牙根面和上皮面。牙密螺旋体主要分布于牙周袋的中间区，在此之前，我们发现E.corrodens 1073具有一种细胞相关凝集素样物质（Cell-associated lectin-like substance，EcLS），它在牙周病相关细菌粘附于各种细胞表面的过程中发挥作用。用抗EcLS单克隆抗体的免疫电镜研究表明，EcLS定位于外膜的外层空间，而不是在细胞表面的附属物，如菌毛，许多细菌具有粘附素。当与来自BALB B/c小鼠的B淋巴细胞一起培养时，发现该EcLS具有有效的促有丝分裂活性。EcLS不仅刺激小鼠B淋巴细胞增殖，而且还刺激其分化为抗体分泌细胞。这些研究结果表明，EcLS可以刺激宿主细胞，并不只是介导宿主细胞和E. corrodens之间的粘附。少

项目成果

H.Nakae et al.: "Mitogenic stimulation of murine B lymphocytes by the N-acetyl-D-galactosamine specific bacterial lectin-like substance from Eikenella corrodens" FEMS Microbiology Letters. 116 in press. (1993)

H.Nakae 等人：“来自腐蚀艾肯菌的 N-乙酰基-D-半乳糖胺特异性细菌凝集素样物质对小鼠 B 淋巴细胞的有丝分裂刺激”FEMS 微生物学快报。

野杁 由一郎ら: "ヒト歯肉縁下プラークの研究ー歯根面関連プラークの免疫組織学的検索ー" 日本歯周病学会誌. 34,春季特別号. 101- (1992)

Yuichiro Nogi 等人：“人类龈下菌斑的研究 - 牙根相关菌斑的免疫组织化学搜索”日本牙周病学会杂志 34，春季特刊 101-（1992 年）。

H.Nakae et al.: "Production and characterization of monoclonal antibodies against abcterial lectin of Eikenella corrodens." J.Periodont.Res.28. 404-410 (1993)

H.Nakae 等人：“针对 Eikenella corrodens 细菌凝集素的单克隆抗体的生产和表征。”

野杁 由一郎ら: "ヒト歯周ポケットにおける歯周病関連細菌の局在性の検索" 日本歯周病学会誌. 35,春季特別号. (1993)

Yuichiro Nogi 等人：“寻找人类牙周袋中牙周病相关细菌的定位”，日本牙周病学会杂志，第 35 期，春季特刊（1993 年）。

S.Ebisu: "Periodontal diagnosis-Currently methods and future direction for diagnosing periodontal diseases" Dental Outlook. 79(4). 857-868 (1992)

S.Ebisu：“牙周诊断 - 诊断牙周疾病的当前方法和未来方向”《牙科展望》。