A study on host-parasite interactions in human periodontal pockets.

用扫描电子显微镜观察了18颗重度成人牙周炎患牙的龈下菌斑。在牙周袋的根尖1/3区域，有几种细菌聚集在一起，呈糖唇状结构，由网状和粘液结构组成。在所谓的无菌斑区，观察到两种类型的细菌黏附：细菌细胞壁与牙根表面直接接触，微生物通过糖衣网状材料黏附在根面上。在知情同意后，30颗重度晚期成人牙周炎患者的牙齿拔除后，用4种牙周病相关细菌的特异性抗体扫描免疫电子显微镜观察无菌斑区。事实上，在检查的7个样本中，有4个样本在无菌斑区域发现了牙龈卟啉单胞菌，在9个样本中有5个样本检测到齿密螺旋体。中间普氏菌发生在o…在所检测的七个样品中只有一个样品，在七个样品中有四个样品被鉴定为粘性放线菌。二次电子显像法检测到的无菌斑区域的螺旋体和杆状菌半数以上与背散射电子显像法检测的4种特异性抗体均不呈阳性反应。免疫组织化学检测了牙周炎组织中T细胞和B细胞的密度和分布，并从牙周组织破坏的角度分析了它们之间的关系。T细胞在低密度浸润液中出现，并随着总浸润物的增多而逐渐增多，而B细胞在小浸润物中不出现，但随着浸润物数目的增加而迅速增加。成纤维细胞数与浸润液总数及B细胞数呈负相关，与T细胞数无相关性。提示T细胞在炎症开始时即进入炎症部位，随着炎症的发展逐渐增多，而B细胞则位于T细胞后面，随着炎症的发展，B细胞的数量迅速增加。较少

We observed subgingival plaque in 18 severe adult-periodontitis affected teeth by scanning electron microscopy. In the apical one-third zone of periodontal pockets, several bacterial species aggregated with glycocalyx-like structures, which comprised net-like and mucoidal structure. In the so-called "plaque-free" zone, two types of bacterial adherence were observed : bacterial cell walls directly contacted with the root surface, and microorganisms adhered to the root surface mediated by the glycocalyx net-like materials.After Providing informed consent, 30 teeth with severe advanced adult periodontitis were extracted, and the plaque-free zones were investigated with specific antibodies against four periodontal diseaseassociated bacteria by a scanning immunoelectron microscopy method. Porphyromonas gingivalis was actually found in the plaque-free zones in four of the seven samples examined, and Treponema denticola was detected in five of nine samples. Prevotella intermedia occurred in o … More nly one of the seven samples examined, and Actinomyces viscosus was identified in four of the seven samples. More than half of the number of the spirochetes and rods detected by secondary electron imaging in the plaque-free zones did not react positively with the four specific antibodies examined by back-scattered electron imaging. This suggests that several unidentified microorganisms were present in the plaque-free zones in addition to the above four bacterial species.We also examined immunohitologically the cell densities and distribution of T and B cells in periodontitis, and analyzed their relationship in terms of periodontal tissue destruction. T cells appeared in the low density of infiltrates and increased gradually with the number of total infiltrates, whereas B cells did not appear in the small infiltrates but increased rapidly with the number of infiltrates. The number of fibroblastic cells showed negative correlation with that of the total infiltrates and B cells, but it did not correlate with the number of T cells. These findings suggest that the T cells infiltrated into the imflamed sites at the onset and increased gradually with the development of inflammation, and that B cells infiltrated behind the T cells and their number increased rapidly with inflammation. Less