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Treatment of dentin hypersensitivity by calcium phosphate precipitation method.

磷酸钙沉淀法治疗牙本质过敏。

基本信息

批准号：
09557152
负责人：
EBISU Shigeyuki
金额：
$ 7.74万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

We have proposed calcium phosphate precipitation (CPP) method which occluded dentinal tubules with apatitic mineral for the treatment of dentin hypersensitivity. In this study, we aimed to evaluate occluding ability of CPP method in vivo using vital teeth of beagle dogs. Class V cavities were prepared in cervical of molars and canines in beagle dogs. Class V cavities were prepared in cervical of molars and canines in beagle dogs, then the teeth were etched with 50% citric acid for 2 min to open dentinal tubules. CPP solution (1M CaHPOィイD24ィエD2・2HィイD22ィエD2O, 2M HィイD23ィエD2POィイD24ィエD2) and post-treatment solution (1M NaHCOィイD23ィエD2, 0.3M NaF) were applied to the teeth with cotton swab. The teeth were divided into two groups, (1) plaque control made by brushing, and (2) nonplaque control group. Dentin biopsy was performed to obtain dentin specimens using cylindrical diamond point immediately after treatment and seven days after treatment with CPP method. The surface and fractured surface of the specimens were observed by scanning electron microscopy. We found that dentinal tubules were occluded homogeneously and completely with apatitic precipitate up to 5 μm from the dentin surface when CPP treatment was made. In non-plaque control group, the dentinal tubules became open seven days after CPP treatment. In contrast, most of the dentinal tubules were still occluded with the precipitate in plaque control group. We concluded that CPP method is useful for the treatment of dentin hypersensitivity when proper plaque control was made. In the other words, plaque control plays a key role for the duration of the dentin hypersensitivity treatment with CPP method.

我们提出磷酸钙沉淀法（CPP），用磷灰质矿物堵塞牙本质小管治疗牙本质过敏。在这项研究中，我们的目的是评估CPP方法在体内的咬合能力，使用比格犬的生牙。在比格犬的臼齿颈部和犬齿处制备了V类空腔。在beagle犬的臼齿颈和犬齿处制备V类空腔，然后用50%柠檬酸蚀刻2 min，打开牙管。用棉签将CPP溶液（1M CaHPO D24 D22 D2O, 2M H D23 D2PO D24 D2）和后处理溶液（1M NaHCO D23 D2, 0.3M NaF）涂抹在牙齿上。将牙齿分为两组，(1)通过刷牙产生菌斑的对照组和(2)没有菌斑的对照组。治疗后即刻和治疗后第7天分别用圆柱菱形点进行牙本质活检，获得牙本质标本。采用扫描电镜对试样表面和断口进行了观察。我们发现，CPP处理后，牙本质表面5 μm范围内的牙本质小管被灰质沉淀均匀地完全堵塞。无菌斑对照组，CPP治疗7天后牙本质小管开放。相比之下，牙菌斑对照组大部分牙本质小管仍被沉淀物堵塞。我们认为CPP方法在牙本质过敏的治疗中是有效的，只要对菌斑进行适当的控制。换句话说，牙菌斑的控制对CPP治疗牙本质过敏的持续时间起着关键作用。