Treatment of dentin hypersensitivity by calcium phosphate precipitation method.

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We have proposed calcium phosphate precipitation (CPP) method which occluded dentinal tubules with apatitic mineral for the treatment of dentin hypersensitivity. In this study, we aimed to evaluate occluding ability of CPP method in vivo using vital teeth of beagle dogs. Class V cavities were prepared in cervical of molars and canines in beagle dogs. Class V cavities were prepared in cervical of molars and canines in beagle dogs, then the teeth were etched with 50% citric acid for 2 min to open dentinal tubules. CPP solution (1M CaHPOィイD24ィエD2・2HィイD22ィエD2O, 2M HィイD23ィエD2POィイD24ィエD2) and post-treatment solution (1M NaHCOィイD23ィエD2, 0.3M NaF) were applied to the teeth with cotton swab. The teeth were divided into two groups, (1) plaque control made by brushing, and (2) nonplaque control group. Dentin biopsy was performed to obtain dentin specimens using cylindrical diamond point immediately after treatment and seven days after treatment with CPP method. The surface and fractured surface of the specimens were observed by scanning electron microscopy. We found that dentinal tubules were occluded homogeneously and completely with apatitic precipitate up to 5 μm from the dentin surface when CPP treatment was made. In non-plaque control group, the dentinal tubules became open seven days after CPP treatment. In contrast, most of the dentinal tubules were still occluded with the precipitate in plaque control group. We concluded that CPP method is useful for the treatment of dentin hypersensitivity when proper plaque control was made. In the other words, plaque control plays a key role for the duration of the dentin hypersensitivity treatment with CPP method.