Fundamental Study on Membrane Surface Chemistry for Ion-Selective Electrodes

离子选择电极膜表面化学基础研究

• 批准号: 03453038
• 负责人: UMEZAWA Yoshio
• 金额: $ 4.1万
• 依托单位: The University of Tokyo (1992)Hokkaido University (1991)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03453038/
• 关键词: Ion-Selective Electrode; Charge Separation; Multitopic Recognition; ATP Sensor; GMP Sensor; Dopamine Sensor; Liquid Membrane; Solid Membrane; 膜界面化学; 脂質二分脂膜; 協同効果; Donnan failure; 脂質二分子膜

In this study, some atomic/molecular level mechanisms of charge-separation chemistry taking place at the surface of solid, liquid, and molecularlayr membranes have been clarified. Also, several new types of ion sensors have been developed.Major results obtained are as follows :1. As new modes of ionic recognition for developing ion sensors, electrostatic, complementary base-pairing, multitopic hydrogen bondings, inclusion into a hydrophobic cavity, pi-pi interations or multitopic recognitions using more than one type of recongnition processes are all exploited.2. On the basis of the above studies, several new ion sensors for bioactive organic ions including ATP^<4->・GMP^<2->・GTP^<4->・ and dopamine are developed.3. FT-IR ATR and optical second harmonic generation (SHG) are used to directly observe the permselective processes at one mum (IR-ATR)-and Debye-length (SHG)-level depth profiles at the surfaces of ionophore incorporated liquid membrane ion-selective electrodes. Also, ion scattering spectroscopy gave top-atomic-layr information at the surface of crystal-face-specific CdS single crystal based ionselective electrodes.

在这项研究中，一些原子/分子水平的电荷分离化学发生在固体，液体和分子层膜的表面机制已经澄清。同时，还研制了几种新型的离子传感器，主要结果如下：1。作为开发离子传感器的新的离子识别模式，静电、互补碱基配对、多主题氢键、包含到疏水腔中、π-π相互作用或使用多种类型的再识别过程的多主题再识别都被开发。在上述研究的基础上，开发了几种新型的生物活性有机离子传感器，包括ATP^<4->·GMP^<2->·GTP^<4->·和多巴胺.利用傅里叶变换红外光谱（FT-IR ATR）和光学二次谐波发生（SHG）技术，在离子载体结合的液膜离子选择电极表面直接观察了选择性渗透过程的1 μ m（IR-ATR）和Debye长度（SHG）水平深度分布。此外，离子散射光谱给出了顶原子层的信息在晶体面特定的CdS单晶基离子选择性电极的表面。

项目成果

K.Tohda, M.Tange, K.Odashima, Y.Umezawa, H.Furuta, and J.L.Sessler: "Liquid Membrane Electrode for Guanosine Nucleotides Using a Cytosine-Pendant Triamine Host as the Sensory Element" Anal.Chem.64. 960-964 (1992)

K.Tohda、M.Tange、K.Odashima、Y.Umezawa、H.Furuta 和 J.L.Sessler：“使用胞嘧啶悬垂三胺宿主作为感觉元件的鸟苷核苷酸液体膜电极”Anal.Chem.64。

K.Tohda: "Liquid Membrane Electrodes for Nucleotides Based on Sapphyrin,Cytosine-Pendant Triamine And Neutral Cytosine Derivative as Sensory Elements" Sensors and Actuators.

K.Tohda：“基于蓝宝石、胞嘧啶悬垂三胺和中性胞嘧啶衍生物作为感觉元件的核苷酸液膜电极”传感器和执行器。

K.Sugao,M.Sugaware,H.Minami,M.Uto,Y.Umezawa: ""Na^+/D-Glucose Cotransporter Based Bilayer Lipid Membrane Sensor for D-Glucose"" Anal.Chem.65. 365-369 (1993)

K.Sugao、M.Sugaware、H.Minami、M.Uto、Y.Umezawa：“基于 Na^ /D-葡萄糖协同转运蛋白的 D-葡萄糖双层脂质膜传感器””Anal.Chem.65。

Y.Adachi, M.Sugawara, K.Taniguchi, Y.Umezawa: "Na^-, K^--ATPase-Based Bilayr Lipid Membrane Sensor for Adenosine 5'-Triphosphate" Anal.Chim.Acta. 281. 577-584 (1993)

Y.Adachi、M.Sukawara、K.Taniguchi、Y.Umezawa：“基于 Na^-、K^--ATP 酶的双层脂质膜传感器，用于 5-三磷酸腺苷”Anal.Chim.Acta。

K.Odashima,M.Kotato,M.Sugawara,and Y.Umezawa: ""Voltammetric Study on a Condensed Monolayer of a Long Alkyl Cyclodextrin Derivative as a Channel Mimetic Sensing Membrane"" Anal.Chem.65. 927-936 (1993)

K.Odashima、M.Kotato、M.Sugara 和 Y.Umezawa：“长烷基环糊精衍生物的凝聚单层作为通道模拟传感膜的伏安研究”Anal.Chem.65。