Chemically modified STM tips for molecular imaging of DNA and cytochrome c

用于 DNA 和细胞色素 c 分子成像的化学修饰 STM 探针

• 批准号: 13440219
• 负责人: UMEZAWA Yoshio
• 金额: $ 11.07万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13440219/
• 关键词: STM; DNA; carbon nanotube; SAM; surface analysis; chemically modified tips; Hydrogen bonding interaction; nucteotides; 化学的相互作用; 科学修飾探針

Cheniistry-iacilitated intermolecular electron tunneling is another approach to molecular imaging that is reported herein. The chemical imaging was obtained from a distinctive chemical affinity between the imaging tip of scanning tunneling microscopy (STM) and the substrate that afters the tunneling current. This was achieved by tailored chemical modification of the STM tips. When chemically modified tips are used for STM measurements, contrast enhancements at specific regions in STM images occur on the basis of hydrogen bond, charge-transfer, and metal-coordination interactions, and as a result, allowed to detect not only the distribution of specific chemical species and functional groups but also the orientation of functional groups. The contrast enhancements reflect the increase in a tunneling current due to the overlap of electronic wave functions induced by the chemical interactions between tip and sample. The molecular imaging of oligonucleotides was achieved therefrom.

化学激发的分子间电子隧穿是本文报道的另一种分子成像方法。化学成像是通过扫描隧道显微镜(STM)的成像尖端与隧道电流之后的衬底之间的一种独特的化学亲和力获得的。这是通过对STM尖端进行量身定制的化学修饰实现的。当化学修饰的TIP用于STM测量时，STM图像中特定区域的对比度增强基于氢键、电荷转移和金属配位相互作用，因此不仅可以检测特定化学物种和官能团的分布，还可以检测官能团的取向。对比度增强反映了针尖和样品之间的化学相互作用引起的电子波函数重叠导致的隧道电流的增加。由此实现了寡核苷酸的分子成像。

项目成果

T.Nishino, T.Ito, Y.Umezawa: "Carbon Nanotube Scanning Tunneling Microscopy Tips for Chemically Selective Imaging"Anal. Chem. 74. 4275-4278 (2002)

T.Nishino、T.Ito、Y.Umezawa：“用于化学选择性成像的碳纳米管扫描隧道显微镜技巧”分析。

T.Nishino, P.Buhlmann, T.Ito, Y.Umezawa: "Scanning tunneling microscopy with chemically modified tips : orientation-sensitive observation of ether oxygens"Surface Science. 490,No 1-2. L579-L584 (2001)

T.Nishino、P.Buhlmann、T.Ito、Y.Umezawa：“带有化学修饰尖端的扫描隧道显微镜：醚氧的方向敏感观察”表面科学。

Y.Umezawa: "Seeing What Was Unseen. New Analytical Methods for Molecular Imaging"The Chemical Record. 3. 22-28 (2003)

Y.Umezawa：“看到看不见的东西。分子成像的新分析方法”化学记录。

T, Nishino, P.Buhlmann, T.Ito, Y.Umezawa: "Discrimianation of Functional groups with Scanning Tuuneling Microscopy Using Chemically Modified Tips Recognition of Ether Oxygens Through Hydrogen Bond Interactions"Physical Chemistry Chemical Physics. 3. 1867-

T、Nishino、P.Buhlmann、T.Ito、Y.Umezawa：“通过扫描调谐显微镜使用化学修饰尖端通过氢键相互作用识别醚氧来区分官能团”物理化学化学物理。

T.Nishino, T.Ito, Y.Umezawa: "Carbon Nanotube STM Tips for Chemically Selective Imaging"Anal. Chem.. 74,No.16. 4275-4278 (2002)

T.Nishino、T.Ito、Y.Umezawa：“用于化学选择性成像的碳纳米管 STM 尖端”分析。