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Study on the regulation of a gene expressed solely in embryonic stage

仅在胚胎阶段表达的基因的调控研究

基本信息

批准号：
03454022
负责人：
YASUGI Sadao
金额：
$ 3.97万
依托单位：
Tokyo Metoropolitan University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

项目摘要

The aim of the present study is to elucidate the regulation mechanisms of gene expression restricted spacio-temporally during the course of animal embryogenesis. We tried to reveal the regulation mechanisms of expression of embryonic chicken pepsinogen (ECPg), which is known to be expressed only in the epithelial cell of embryonic proventriculus. The main results obtained can be summarized as follows :(1) The experimental embryological studies showed that epithelial cells of esophagus, proventriculus and gizzard of have potency to express ECPg under appropriate conditions and inhibitory influences of esophageal and gizzard mesenchymes ensure the restricted expression of ECPg gene in the normal course of development. Also, the expression of a sort of keratin molecule is regulated by the action of the mesenchyme. (2) The demethylation of DNA of ECPg gene is involved in the organ-specific expression of the gene. (3) We demonstrated that the influences of proventricular and gizzard mesenchymes are mediated to the expression of ECPg gene via the l kb sequence of 5' region of the gene.From the above mentioned results, we can now conceive a scenario leading to the restricted expression of ECPg gene. In the early period of development, the endoderm composing the digestive tract is divided into two parts, with regard to the potency express ECPg gene. The realization of ECPg expression thereafter is brought about by fine turning of the mesenchymal influences. The dementhylation of ECPg gene can be thought as a kind of double assurance. The identification of trans-acting factors which bind to the 5' region of ECPg gene and the regulation of expression of these factors by mesenchymal influences will be important problems in future.

本研究旨在阐明动物胚胎发育过程中基因表达的时空调控机制。鸡胚胃蛋白酶原（embryonic chicken pepsinogen，ECPg）只在鸡胚前胃上皮细胞中表达，本研究试图揭示ECPg表达的调控机制。（1）实验胚胎学研究表明，在适当的条件下，食管、腺胃和肌胃上皮细胞具有表达ECPg的潜能，食管和肌胃间充质的抑制作用保证了ECPg基因在正常发育过程中的限制性表达。此外，一种角蛋白分子的表达受间充质的作用调节。(2)ECPg基因的DNA去甲基化参与了该基因的器官特异性表达。(3)结果表明，腺胃和肌胃间充质对ECPg基因表达的影响是通过ECPg基因5'端1kb序列介导的，由此推测ECPg基因表达受限制的可能性。在发育的早期，组成消化道的内胚层分为两部分，表达ECPg基因的能力。此后ECPg表达的实现是通过间充质影响的精细转向而实现的。ECPg基因的去孟甲基化可以被认为是一种双重保证。鉴定与ECPg基因5'区结合的反式作用因子，并通过间质影响调节这些因子的表达将是未来的重要问题。