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Function analysis of soxgenes in the development and differentiation of digestion

soxgenes在消化发育和分化中的功能分析

基本信息

批准号：
09044233
负责人：
YASUGI Sadao
金额：
$ 4.99万
依托单位：
TOKYO METOROPOLITAN UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

The present study intended to clarify the functions of Sox genes, which recently become known to play important roles in various processes of organogenesis, in the development and differentiation of vertebrate digestive organs.Sox genes encode transcription factors having HMG domain and more than 20 genes have been cloned so far. We paid special attention to cSox2 and cSox21, which are expressed in the gut epithelium of chicken embryo. We analyzed in the present study their expression patterns, regulation of these genes by the mesenchyme, and functional analysis using gene transduction methods. Both genes are expressed in the epithelium of anterior digestive organs such as esophagus, proventriculus and gizzard. Their expression ceased when embryonic morphogenesis and differentiation come to end. Their posterior boundary of expression coincided with the anterior boundary o CdxA, a gene specifically expressed in the intestinal epithelium. Moreover, their expression was very low in the epithelium of newly formed proventricular glands.Expression of cSox2 and cSox21 was shown to be regulated by the mesenchyme. When the intestinal epithelium was cultivated with gizzard mesenchyme some epithelial cells became positive to cSox2 and cSox21. Conversely, the expression of both genes was suppressed in stomach epithelium cultivated with intestinal mesenchyme.Introduction and forced expression of cSox2 and cSox21 into intestinal epithelium never resulted in the suppression of CdxA, suggesting that some partner molecules are necessary for the suppression of target genes by cSox2 and cSox21 gene production.Thus, in the present study, possible involvement of cSox2 and cSox21 in the organogenesis of the digestive organs was demonstrated. In addition, the basic data for further analysis of their function were accumulated.

Sox基因是脊椎动物消化器官发育和分化过程中的重要调控基因，目前已克隆的Sox基因有20多个，它们编码具有HMG结构域的转录因子。我们特别关注了cSox 2和cSox 21，它们在鸡胚肠上皮中表达。在本研究中，我们分析了它们的表达模式，这些基因的间充质调节，并使用基因转导方法进行功能分析。这两个基因在前消化器官如食管、前胃和砂囊的上皮中表达。当胚胎形态发生和分化结束时，它们的表达停止。他们的后边界的表达与前边界的CdxA，一个基因特异性表达在肠上皮细胞。cSox 2和cSox 21的表达受间充质的调节。当肠上皮细胞与肌胃间充质培养时，一些上皮细胞对cSox 2和cSox 21呈阳性。相反，在与肠间充质培养的胃上皮中，这两种基因的表达均受到抑制。将cSox 2和cSox 21引入肠上皮并强制表达从未导致CdxA的抑制，这表明某些伴侣分子对于cSox 2和cSox 21基因的产生抑制靶基因是必需的。因此，在本研究中，证明了cSox 2和cSox 21可能参与消化器官的器官发生。为进一步分析其功能积累了基础数据。