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Molecular identification and functional differentiation of stem cells in the digestive organs

消化器官干细胞的分子鉴定和功能分化

基本信息

批准号：
18570204
负责人：
YASUGI Sadao
金额：
$ 2.59万
依托单位：
Teikyo Heisei University (2007)Tokyo Metropolitan University (2006)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18570204/
关键词：
Development and differentiation Regenerative medicine Stem cell Digestive organ Chicken embryo 細胞培養遺伝子発現 PPAR 形態形成細胞分化

项目摘要

The present study revealed following points. (1) Peroxysome proliferators activated receptors (PPARs) are expressed in different manners in the development of the digestive organs of chicken embryos. PPARgamma is expressed exclusively in the luminal epithelium of the proventriculus and is shown to inhibit gland formation when overexpressed in the epithelium together with indomethacin (agonist). (2) We established the culture system to cultivate epithelial cells of the embryonic proventriculus. Under the conditions utilized, cells differentiate to different degree according to the nature of factors added to the medium. (3) With the system described above, genes which are expressed from the early stages in the normal development are also expressed without any factors. Those expressed around the time of gland formation requires some mesenchymal factors to be expressed in the culture. This culture system will provide us the convenient system to assay the differentiation potency of the stem cells. (4) We tried to establish a system in which chicken embryonic germ cells are co-cultured with mesenchymal or epithelial cells of the proventriculus. To examined the potency to differentiate into digestive organ cells. We cultivated PGCs with some growth factors and obtained cells espressing SSEA-1, a marker of undeifferentiated cells. We collected these cells by MACS and cultivated the on the proventriculus. After several days, we detected expression of some differentiation marker genes in the cells. This system seemed appropriate to examine the differentiation potency of the stem cells towards cells of the digestive organs.

本研究揭示了以下几点。(1)过氧化物酶体增殖物激活受体（PPARs）在鸡胚消化器官发育过程中以不同方式表达。PPARgamma仅在前胃的腔上皮中表达，并且当与吲哚美辛（激动剂）一起在上皮中过表达时，显示出抑制腺体形成。(2)建立了胚胎前胃上皮细胞的培养体系。在所使用的条件下，细胞根据添加到培养基中的因子的性质分化到不同程度。(3)利用上述系统，在正常发育的早期阶段表达的基因也在没有任何因子的情况下表达。那些在腺体形成前后表达的细胞需要一些间充质因子在培养物中表达。该培养体系将为干细胞分化潜能的检测提供方便的实验手段。(4)本研究尝试建立鸡胚生殖细胞与腺胃间充质细胞或腺胃上皮细胞共培养体系。检测其向消化器官细胞分化的潜能。我们用一些生长因子培养PGCs，并获得表达SSEA-1的细胞，SSEA-1是未分化细胞的标志物。我们用MACS收集这些细胞，并在前胃上培养。几天后，我们检测到细胞中一些分化标记基因的表达。该系统似乎适合于检查干细胞向消化器官细胞的分化潜能。