Dynamics of signal transduction in the system of organogenesis and regeneration

器官发生和再生系统中信号转导的动力学

• 批准号: 13044002
• 负责人: YASUGI Sadao
• 金额: $ 327.3万
• 依托单位: Tokyo Metropolitan University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13044002/
• 关键词: organogenesis; tissue interaction; transduction; growth factor; transcrition factor; cell differentiation; regenerative medicine; マイクロアレイ; シグナル伝達; 細胞・組織; 再生医学; 発生・分化; 再生; 遺伝子カスケード; 情報伝達; 形態形成物質

In 2005, the networks of gene expressions in the formation of many organ systems have been analyzed. In planarians, two more noggin-like genes were identified and role of these genes in the establishment of body axis was demonstrated (Agata). In amphibians, the function of MA35, a newly found gene involved in the heart formation, was examined. Also Wnt signaling cascade in the early stages of development was extensively studied. These investigations opened a new horizon of organogenesis research in amphibians (Asashima). Co-factors necessary for the transcription of TbxS were identified. Knockdown of some of them in zebrafish showed close relationship between transcriptional control and cytoskeletal system (Ogura). In the course of cartilage formation during the limb development, Hox genes determine the positional characteristics of pre- cartilage cell mass. It was also found that ephrin-b1 and EphB2 function as downstream cell adhesion factors (Kuroiwa). In Drosophila visual system, the suppression of JAK/STAT signal resulted in the shift of lamina neuron to medulla neuron. The JAK/STAT and Notch signals are suggested to regulate multistep differentiation of neurons from the neural stem cells (Tabata). Wnt5a was found to regulate cell differentiation of the epithelium in the glandular stomach of the chicken embryo. Overexpression and suppression of this gene resulted in the overexrpession and/or ectopic expression of some region-specific marker genes of the epithelium. (Yasugi)

2005年，许多器官系统形成中的基因表达网络被分析。在真涡虫中，又发现了两个类似头蛋白的基因，并证明了这些基因在体轴建立中的作用（Agata）。在两栖动物中，研究了新发现的参与心脏形成的基因MA 35的功能。此外，Wnt信号级联在发育的早期阶段进行了广泛的研究。这些研究开辟了两栖动物器官发生研究的新视野。确定了转录TbxS所需的辅因子。在斑马鱼中敲除其中的一些基因表明转录调控与细胞骨架系统（Ogura）密切相关。在肢体发育的软骨形成过程中，Hox基因决定了前软骨细胞团的位置特征。还发现肝配蛋白-b1和EphB 2作为下游细胞粘附因子发挥功能（Kuroiwa）。在果蝇的视觉系统中，JAK/STAT信号的抑制导致了板层神经元向髓质神经元的移位。JAK/STAT和Notch信号被认为调节神经干细胞（Tabata）向神经元的多步分化。发现wnt 5a调节鸡胚腺胃上皮细胞的分化。该基因的过表达和抑制导致上皮细胞的某些区域特异性标记基因的过度表达和/或异位表达。（Yasugi）

项目成果

動脈内皮細胞の判別方法

如何识别动脉内皮细胞

• 发表时间: 2004

Inhibition of the Canonical Wnt Signaling Pathway in Cytoplasm: a Novel Property of the Carboxyl Terminal Domains of Two Xenopus ELL Genes

• DOI: 10.2108/zsj.21.407
• 发表时间: 2004-04
• 作者: K. Sakurai;T. Michiue;A. Kikuchi;M. Asashima

Temporal and spatial manipulation of gene expression in Xenopus embryos by injection of heat shock promoter-containing plasmids

• DOI: 10.1002/dvdy.20233
• 发表时间: 2005-02-01
• 期刊: DEVELOPMENTAL DYNAMICS
• 影响因子: 2.5
• 作者: Michiue, T;Asashima, M
• 通讯作者: Asashima, M

CD151 regulates epithelial cell-cell adhesion through PKC- and Cdc42-dependent actin cytoskeletal reorganization.

• DOI: 10.1083/jcb.200301075
• 发表时间: 2003-10-13
• 期刊: JOURNAL OF CELL BIOLOGY
• 影响因子: 7.8
• 作者: Shigeta, Masaki;Sanzen, Noriko;Ozawa, Masayuki;Gu, Jianguo;Hasegawa, Hitoshi;Sekiguchi, Kiyotoshi
• 通讯作者: Sekiguchi, Kiyotoshi

Xantivin suppresses the activity of EGF-CFC genes to regulate nodal signaling.

• DOI: 10.1387/ijdb.041805kt
• 发表时间: 2004-07
• 期刊: The International journal of developmental biology
• 作者: K. Tanegashima;Yoshikazu Haramoto;C. Yokota;Shuji Takahashi;M. Asashima