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Cell-type-specific expression of genes encoding subunits of plant mitochindrail ATP synthase and their coordiantion

植物线粒体ATP合酶亚基编码基因的细胞类型特异性表达及其协调

基本信息

批准号：
04454073
负责人：
NAKAMURA Kenzo
金额：
$ 4.29万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454073/
关键词：
Plant genes Plant mitochondria F1FOATPase Gene expression

项目摘要

F_1-ATPase of plant mitochondria is composed of six subunits of alpha, beta, gamma, delta, delta', and epsilon. Subunits other than alpha, that is encoded by mitochondrial genome, are encoded by nuclear genes. We have isolated nuclear genes for sigma' and epsilon subunits from sweet potato(Ipomoea batatas) or closely related wild specied (Ipomoea trifida), and determined their nucleotide sequences. Similar the the sigma subunit, these subunits were encoded by multiple genes in the genome. Nuclear genes for the sigma' subunit contained one intron, and exons of these two genes were almost identical to each other. On the other hand, nucleotide sequences of exons from two genes for the sigma subunit were only 81.6% identical to each other. We constructed fusion genes in which promoter fragments from two nuclear genes for the delta subunits, that had been characterixed proviously, were fused to the GUS coding sequence. These fusion genes showed constitutive expression in all of the organs examined. Histochemical examination of expression of fusion genes revealed tha their expression was most strong in phloem tissues of leaves and stems that contain mitochondria-enriched compoanion cells. Between the two fusion genes, the level of expression in roots differed significantly. Futhermore, expression of the fusion gene during the growth of transformed cells of tobacco in suspension culture was also significantly different between the two promoters. These results suggested that multiple genes for nuclear subunits of F_1 ATPase are regulated differently.

植物线粒体f_1 - atp酶由α、β、γ、δ、δ′和epsilon六个亚基组成。除了α以外的亚基，由线粒体基因组编码，是由核基因编码的。从甘薯（Ipomoea batatas）和近缘野生甘薯（Ipomoea trifida）中分离出sigma'和epsilon亚基的核基因，并确定了它们的核苷酸序列。与sigma亚基类似，这些亚基是由基因组中的多个基因编码的。sigma亚基的核基因含有一个内含子，这两个基因的外显子几乎相同。另一方面，两个基因的sigma亚基外显子核苷酸序列的相似性仅为81.6%。我们构建了融合基因，将两个核基因的启动子片段融合到GUS编码序列中。这些融合基因在所有被检查的器官中都表现出组成性表达。融合基因表达的组织化学检测表明，融合基因在含有富含线粒体复合物细胞的叶和茎韧皮部组织中表达最强烈。两种融合基因在根中的表达量差异显著。此外，两种启动子在烟草悬浮培养转化细胞生长过程中融合基因的表达也有显著差异。这些结果表明F_1 atp酶核亚基的多个基因受到不同的调控。