NU-UCB Cooperative Program on plant Molecular Biology

名古屋大学(NU)和加州大学伯克利分校(UCB)的成员和非成员在名古屋大学(NU)和加州大学伯克利分校(UCB)的“NU-UCB植物分子生物学合作计划”下进行了合作和激励性的讨论，取得了以下进展：1)库活动和钙信号的调节该计划的重点之一是钙信号在库-源相互作用调节中的作用。Nakamura和Sugiyama(Nu)分别证明钙信号参与糖诱导的基因表达和硝酸盐对基因表达的调控。琼斯(UCB)发现大麦种子糊粉层的功能依赖于特定的钙调蛋白亚型，中村部分从烟草叶片中纯化了一种与质膜密切相关的新的钙依赖蛋白激酶(CDPK)亚型。2)来源活性和质体基因表达的调节Sugiura(Nu)和Grulissem(Ucb)在TH…上传递更多的体外系统用于研究叶绿体基因的表达，Sugiura开发了体外翻译系统，Gruissem开发了转录成熟的体外系统。3)植物液泡的功能Maeshima(Nu)和Jones(UCB)合作研究了Jones在大麦种子糊粉细胞中发现的新的液泡状结构的免疫细胞化学特征。4)拟南芥的分子遗传学从T-DNA标记的突变体中，Fischer(UCB)发现Bell-1编码了一种对胚胎发生至关重要的同源结构域因子，Mchida(Nu)发现NADPH-氧化酶在生长控制中起着重要作用。Mchida还发现了一种在受伤后迅速激活的质膜相关激酶，Gruissem(UCB)分离了一个参与肌醇磷酸代谢的基因。5)农业应用Nakamura和Mchida(Nu)帮助Buchanan和Lemaux(UCB)通过Nu构建的载体启动了农杆菌介导的大麦转化。较少

Collaboration and stimulative discussions among members, and non-members, of the Nagoya University (NU) and the University of California, Berkeley (UCB) under the program "NU-UCB Cooperative Program on Plant Molecular Biology", yielded the following progress.1) Regulation of sink activity and calcium-signalingOne of the reseach topics focused in the program was the role of calcium-signaling in the regulation of sink-source interaction. Nakamura and Sugiyama (NU) have shown that calcium-signaling is involved in the sugar-inducible gene expression, and in the nitrate-regulation of gene expression, respectively. Jones (UCB) found that function of aleurone layr of barley seeds depends on specific isoform of calmodulin, and Nakamura partially purified a novel isoform of calcium-dependent protein kinase (CDPK) from tobacco leaves which is tightly associated with the plasma membrane.2) Regulation of source activity and plastid gene expressionSugiura (NU) and Grulissem (UCB) communicated on th … More e in vitro systems to study chloroplast gene expression, and Sugiura developed in vitro translation system and Gruissem developed in vitro system for the maturation of transcripts.3) Function of plant vacuolesMaeshima (NU) and Jones (UCB) collaborated on the immuno-cytochemical characterization of novel vacuole-like structures which Jones found in aleurone cells of barley seeds. Maeshima isolated cDNAs for tonoplast Ca^<2+>/H^+ antiporter.4) Molecular genetics of Arabidopsis thalianaFrom T-DNA tagged mutant lines, Fischer (UCB) found that Bell-1 codes for a homoeodomain factor essential for embryogenesis, and Machida (NU) found that NADPH-oxidase plays an important role in the growth control. Machida also identified a plasma membrane-associated kinase which is rapidly activated after wounding, and Gruissem (UCB) isolated a gene involved in the metabolism of inositol-phosphate.5) Application to agricultureNakamura and Machida (NU) helped Buchanan and Lemaux (UCB) to start Agrobacterium-mediated transformation of barley by vectors developed in NU. Less