NU-UCB Cooperative Program on plant Molecular Biology

NU-UCB植物分子生物学合作项目

• 批准号: 07044189
• 负责人: NAKAMURA Kenzo
• 金额: $ 2.82万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07044189/
• 关键词: Plant genes; Sink and source; Signal transduction; Calcium signaling; Plant vacuoles; Arabidopsis thaliana; Mutants; Ca^<2+>_-シグナル; 胚発生変異株

Collaboration and stimulative discussions among members, and non-members, of the Nagoya University (NU) and the University of California, Berkeley (UCB) under the program "NU-UCB Cooperative Program on Plant Molecular Biology", yielded the following progress.1) Regulation of sink activity and calcium-signalingOne of the reseach topics focused in the program was the role of calcium-signaling in the regulation of sink-source interaction. Nakamura and Sugiyama (NU) have shown that calcium-signaling is involved in the sugar-inducible gene expression, and in the nitrate-regulation of gene expression, respectively. Jones (UCB) found that function of aleurone layr of barley seeds depends on specific isoform of calmodulin, and Nakamura partially purified a novel isoform of calcium-dependent protein kinase (CDPK) from tobacco leaves which is tightly associated with the plasma membrane.2) Regulation of source activity and plastid gene expressionSugiura (NU) and Grulissem (UCB) communicated on th … More e in vitro systems to study chloroplast gene expression, and Sugiura developed in vitro translation system and Gruissem developed in vitro system for the maturation of transcripts.3) Function of plant vacuolesMaeshima (NU) and Jones (UCB) collaborated on the immuno-cytochemical characterization of novel vacuole-like structures which Jones found in aleurone cells of barley seeds. Maeshima isolated cDNAs for tonoplast Ca^<2+>/H^+ antiporter.4) Molecular genetics of Arabidopsis thalianaFrom T-DNA tagged mutant lines, Fischer (UCB) found that Bell-1 codes for a homoeodomain factor essential for embryogenesis, and Machida (NU) found that NADPH-oxidase plays an important role in the growth control. Machida also identified a plasma membrane-associated kinase which is rapidly activated after wounding, and Gruissem (UCB) isolated a gene involved in the metabolism of inositol-phosphate.5) Application to agricultureNakamura and Machida (NU) helped Buchanan and Lemaux (UCB) to start Agrobacterium-mediated transformation of barley by vectors developed in NU. Less

名古屋大学 (NU) 和加州大学伯克利分校 (UCB) 在“NU-UCB 植物分子生物学合作项目”下的成员和非成员之间的合作和促进性讨论取得了以下进展。 1) 库活动和钙信号的调节该项目重点研究的主题之一是钙信号在库源调节中的作用 相互作用。 Nakamura 和 Sugiyama (NU) 已经表明，钙信号传导分别参与糖诱导基因表达和基因表达的硝酸盐调节。 Jones（UCB）发现大麦种子糊粉层的功能依赖于特定的钙调素亚型，Nakamura从烟叶中部分纯化了一种与质膜紧密相关的新型钙依赖性蛋白激酶（CDPK）亚型。 2）来源活性和质体基因表达的调节Sugiura（NU）和Grulissem (UCB) 在体外系统上进行交流，以研究叶绿体基因表达，Sugiura 开发了体外翻译系统，Gruissem 开发了用于转录本成熟的体外系统。3) 植物液泡的功能前岛 (NU) 和琼斯 (UCB) 合作对琼斯在糊粉细胞中发现的新型液泡样结构进行免疫细胞化学表征 大麦种子。 Maeshima分离了液泡膜Ca^2+/H^+反向转运蛋白的cDNA。4)拟南芥的分子遗传学Fischer (UCB)从T-DNA标记的突变系中发现Bell-1编码胚胎发生必需的同源域因子，Machida (NU)发现NADPH氧化酶在生长控制中发挥重要作用。 Machida 还鉴定了一种在受伤后迅速激活的质膜相关激酶，Gruissem (UCB) 分离了一个参与磷酸肌醇代谢的基因。 5) 在农业中的应用Nakamura 和 Machida (NU) 帮助 Buchanan 和 Lemaux (UCB) 开始通过农杆菌介导的大麦转化，该载体是在 努。较少的

项目成果

M.Taniguchi: "Aspartate aminotransferase isozymes in Panicum miliaceum L., an NAD-malic enzyme-type C4 plant." Archives of Biochemistry and Biophysics. 318. 295-306 (1995)

M.Taniguchi：“Panicum miliaceum L.（一种 NAD-苹果酸酶型 C4 植物）中的天冬氨酸转氨酶同工酶。”

R.Hays: "Chloroplast mRNA 3'-end processing by a high molecular weight protein complex is regulated by nuclear encoded RNA binding proteins." EMBO J.15. 1132-1141 (1996)

R.Hays：“高分子量蛋白复合物对叶绿体 mRNA 3 端的加工受到核编码 RNA 结合蛋白的调节。”

I.Suzuki: "Regulation by cyanate of the genes involved in carbon and nitrogen assimilation in the cyanobacterium Synechococcus sp.strain PCC7942." J.Bacteriol.178. 2688-2694 (1996)

I.Suzuki：“氰酸盐对蓝藻聚球藻菌株 PCC7942 中参与碳和氮同化的基因的调节。”

K.M.KLucher: "AINTEGUMENTA gene in Arabidopsis required for ovule and female gametophyte development is related to the floral homeotic gene APETALA2." Plant Cell. 8. 137-153 (1995)

K.M.KLucher：“拟南芥中胚珠和雌配子体发育所需的AINTEGUMENTA 基因与花同源异型基因APETALA2 相关。”

T.Hirose: "Cis-acting elements and trans-acting factors for accurate translation of chloropast psbA mRNAs : development of an in vitro translation system from tobacco chloroplasts." EMBO J.15. 1687-1695 (1996)

T.Hirose：“用于准确翻译叶绿体 psbA mRNA 的顺式作用元件和反式作用因子：开发烟草叶绿体体外翻译系统。”