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Genetic Program Controlling Metabolic Function of the Plant Organ

控制植物器官代谢功能的遗传程序

基本信息

批准号：
06278102
负责人：
NAKAMURA Kenzo
金额：
$ 183.04万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06278102/
关键词：
Plant organ Regulation of gene expression Regulatory genes Transcription factor Signal transduction factor Genetic program Model plants

项目摘要

This project aimed to clarify genetic program controling the metabolic and cellular activities in plant organs. The major research achievements in the 4-year project include ; (1) Isolation of new regulatory factors ; New bZIP factor which mediates ABA-dependent gene expression in seeds (Hattori), and ABA-inducible MYB and MYC factors involved in drought-response (Shinozaki), were isolated. Transcription factors involved in the S phase-specific gene expression were characterized (Meshi). Genes for trimeric G were characterized and its gene-disruptant of rice was isolated (Iwasaki). (2) Isolation of mutants ; Arabidopsis mutants with defects in sugar-responsible gene expression (Nakamura), and a mutant which accumulates methionine and affects seed storage protein (Naito) were characterized. Transposon tagging lines of rice were constructed and various mutants were characterized (Shimamoto). (3) Dynamics of organelle ; Genes for vacuolar processing enzyme involved in programmed cell death (Nishimura), proton-pump and waterchannel (Maeshima), and small G-protein involved in vesicular transport (Sasaki) were isolated and their expression patterns during organ development were characterized. Import redundancy of mitochondrial tRNAs (Ohyama), and cortical cell-death in rice roots were characterized (Uchimiya).

该项目旨在阐明控制植物器官代谢和细胞活动的遗传程序。历时4年的项目主要研究成果包括：（1）新的监管因素的隔离；分离出介导种子中 ABA 依赖性基因表达的新 bZIP 因子 (Hattori)，以及参与干旱响应的 ABA 诱导型 MYB 和 MYC 因子 (Shinozaki)。对参与 S 期特异性基因表达的转录因子进行了表征 (Meshi)。对三聚体 G 的基因进行了表征，并分离了其水稻基因破坏物（Iwasaki）。 (2)突变体的分离；对糖相关基因表达缺陷的拟南芥突变体（Nakamura）和积累蛋氨酸并影响种子储存蛋白的突变体（Naito）进行了表征。构建了水稻的转座子标记系并表征了各种突变体（Shimamoto）。 (3) 细胞器动力学；分离了参与程序性细胞死亡（Nishimura）、质子泵和水通道（Maeshima）的液泡加工酶以及参与囊泡运输（Sasaki）的小G蛋白的基因，并表征了它们在器官发育过程中的表达模式。线粒体 tRNA 的输入冗余 (Ohyama) 和水稻根部皮质细胞死亡的特征 (Uchimiya) 得到了表征。