Sugar-inducible gene expression and vacuolar accumulation of storage proteins
糖诱导基因表达和储存蛋白的液泡积累
基本信息
- 批准号:12138203
- 负责人:
- 金额:$ 18.94万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research on Priority Areas
- 财政年份:2000
- 资助国家:日本
- 起止时间:2000 至 2003
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
By yeast one-hybrid screen with DNA fragment containing two sequence motifs conserved among the sugar-inducible genes of sweet potato tuberous roots, two tobacco cDNAs coding for putative transcription factors with unknown function were isolated. One of them codes for a protein with HD-Zip domain that binds to Box 3 motif and contains activation domain in its C-terminus. Another one codes for a novel class of bZIP factor. Map-based cloning of lbal mutant of Arabidopsis thaliana that shows reduced sugar-inducible expression of Atβ-Amy gene and accumulation of anthocyanin resulted in identification of a mutation that accompany Gly to Glu substitution in a large protein with unknown function. Among 55 mutants that show altered sugar-inducible expression of Spo^<min> : : LUC reporter. we identified hsi2 by map-based cloning. HSI2 was a novel protein with B3 DNA binding domain and analysis of null-mutant and overexpressor indicated that HSI2 is a reptessor of Spo^<min>. By screening of acti … More vation tagging lines of Spo^<min> : : LUC transgenic plant, three clones that express LUC reporter under low-sugar condition were identified. In these mutants, expression of genes encoding bZIP factor, CCT motif protein and AP2 domain protein with unknown functions were elevated by insertion of enhancer. In particular, enhanced expression of the novel bZIP resulted in enhanced expression of some of the endogenous sugar-inducible genes in addition to Spo^<min> : :LUC. By using oligo-microarray covering all genes of Arabidopsis, we identified transcription factor genes whoes expression is rapidly induced or repressed by sugars in a cycloheximide-insensitive manner. Among these. AtbZIP 22 was found to be required for the sugar-inducible expression of PR-1 gene, and the sugar-repressible AtbZIP63 was shown to be involved in the sugar starvation-induced gene expression. We also found that sugar induces expression of a large number of genes involved in ribosome biogenesis, and nucleolin plays an important role in the regulation of ribosome biogenesis. Less
通过酵母单杂交筛选甘薯块根糖诱导基因中保守的2个序列基序,获得了2个编码功能未知的烟草转录因子的DNA。其中一个编码具有HD-Zip结构域的蛋白,该结构域结合Box 3基序,并且在其C-末端含有激活结构域。另一个编码为一类新的bZIP因子。基于图位克隆拟南芥lbaI突变体,该突变体显示糖诱导的Atβ-Amy基因的表达减少和花青素苷的积累,导致鉴定出在功能未知的大蛋白中伴随Gly至Glu取代的突变。在55个突变体中,显示Spo^<min>::LUC报告基因的糖诱导表达改变。我们通过图位克隆鉴定了hsi 2。HSI 2是一个新的具有B3 DNA结合域的蛋白质,对HSI 2的缺失突变和过表达分析表明,HSI 2是Spo^的一个修复子<min>。通过筛选活动, ...更多信息 在Spo^<min>::LUC转基因植株的vation标签系中,鉴定了3个在低糖条件下表达LUC报告基因的克隆。在这些突变体中,编码bZIP因子、CCT基序蛋白和功能未知的AP 2结构域蛋白的基因的表达通过插入增强子而升高。特别地,新bZIP的增强表达导致除了Spo^::LUC之外的一些内源糖诱导基因的增强表达<min>。通过使用覆盖拟南芥所有基因的寡核苷酸芯片,我们鉴定了以环己酰亚胺不敏感的方式被糖快速诱导或抑制表达的转录因子基因。其中。AtbZIP 22是糖诱导型PR-1基因表达所必需的,而AtbZIP 63则是糖抑制型的,参与了糖饥饿诱导的PR-1基因表达。我们还发现糖诱导大量参与核糖体生物合成的基因表达,核仁素在核糖体生物合成的调控中起重要作用。少
项目成果
期刊论文数量(40)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Suzuki, T., Inagaki, S., Nakajima, S.: "A novel Arabidopsis gene TONSOKU is required for proper cell arrangement in root and shoot apical meristems."Plant J., in press. (2004)
Suzuki, T.、Inagaki, S.、Nakajima, S.:“根和芽顶端分生组织中的细胞正确排列需要一种新的拟南芥基因 TONSOKU。”Plant J.,出版中。
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- 影响因子:0
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Yang, H., Matsubayashi, Y., Nakamura, K., Sakagami, Y.: "Diversity of Arabidopsis genes encoding precursors for phytosulfokine, a peptide growth factor"Plant Physiology. 127. 842-851 (2001)
Yang,H.,Matsubayashi,Y.,Nakamura,K.,Sakagami,Y.:“编码植物硫因子(一种肽生长因子)前体的拟南芥基因的多样性”植物生理学。
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- 影响因子:0
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Masuda, T., Fusada, N., Oosawa, N.: "Functional analysis of isoforms of NADPH : protochlorophyllide oxidoreductase (POR), PORB and PORC, in Arabidopsis thaliana."Plant Cell Physiol. 44. 963-974 (2003)
Masuda, T.、Fusada, N.、Oosawa, N.:“拟南芥中 NADPH 亚型的功能分析:原叶绿素氧化还原酶 (POR)、PORB 和 PORC。”植物细胞生理学。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Masuda, T.ら16名: "Functional analysis of isoforms of NADPH : protochlorophyllide oxidoreductase (POR), PORB and PORC, in Arabidopsis thaliana."Plant Cell Physiol.. 44. 963-974 (2003)
Masuda, T. 等 16 位作者:“拟南芥中 NADPH 异构体的功能分析:原叶绿素氧化还原酶 (POR)、PORB 和 PORC。44. 963-974 (2003)
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- 影响因子:0
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鈴木 孝征: "『植物の形づくり』(共著) 論文題目:茎頂と根端の両分裂組織ではたらくTSK遺伝子"共立出版. 325 (2002)
Takayuki Suzuki:“‘植物塑造’(合着)论文标题:TSK 基因在茎尖和根分生组织中发挥作用”Kyoritsu Shuppan 325 (2002)。
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NAKAMURA Kenzo其他文献
NAKAMURA Kenzo的其他文献
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{{ truncateString('NAKAMURA Kenzo', 18)}}的其他基金
High-Throughput Genetic Identification of Regulatory Factors Involved in the Regulation of Seed Oil Storage Using Bioluminescence Automatic Monitoring System
利用生物发光自动监测系统对参与种子油储存调控的调控因子进行高通量遗传鉴定
- 批准号:
23380057 - 财政年份:2011
- 资助金额:
$ 18.94万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Functional analysis of novel transcription factors of Arabidopsis thaliana involved in the regulation of nutrient storage.
拟南芥参与营养储存调节的新型转录因子的功能分析。
- 批准号:
18380065 - 财政年份:2006
- 资助金额:
$ 18.94万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Study of Neutrino Mass and Mixing by Long Baseline Neutrino Oscillations : from K2K to a Next-Generation Experiment
通过长基线中微子振荡研究中微子质量和混合:从 K2K 到下一代实验
- 批准号:
15204023 - 财政年份:2003
- 资助金额:
$ 18.94万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
New Developments of Accelerator Neutrino Experiments
加速器中微子实验新进展
- 批准号:
12440072 - 财政年份:2000
- 资助金额:
$ 18.94万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Flexible Organ Plan in Higher Plants
高等植物的灵活器官计划
- 批准号:
06278101 - 财政年份:1998
- 资助金额:
$ 18.94万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
Transcription Factors Involved in the induction of Jasmonate-Primary Responsive Gene in Plant Cells
植物细胞中参与诱导茉莉酸初级反应基因的转录因子
- 批准号:
10460038 - 财政年份:1998
- 资助金额:
$ 18.94万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Analysis of Protein Transport to the Vacuole in Plants as a Basis for the Production of Usefull Proteins in Higher Plants
植物液泡蛋白质运输分析作为高等植物生产有用蛋白质的基础
- 批准号:
08660102 - 财政年份:1996
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$ 18.94万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
NU-UCB Cooperative Program on plant Molecular Biology
NU-UCB植物分子生物学合作项目
- 批准号:
07044189 - 财政年份:1995
- 资助金额:
$ 18.94万 - 项目类别:
Grant-in-Aid for international Scientific Research
Genetic Program Controlling Metabolic Function of the Plant Organ
控制植物器官代谢功能的遗传程序
- 批准号:
06278102 - 财政年份:1994
- 资助金额:
$ 18.94万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
Regulation of expression of genes coding for subunits of plant mitochondrial F1-ATPase
植物线粒体 F1-ATP 酶亚基编码基因表达的调控
- 批准号:
06660098 - 财政年份:1994
- 资助金额:
$ 18.94万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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植物基因的生与死
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