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Functional analysis of novel transcription factors of Arabidopsis thaliana involved in the regulation of nutrient storage.

拟南芥参与营养储存调节的新型转录因子的功能分析。

基本信息

批准号：
18380065
负责人：
NAKAMURA Kenzo
金额：
$ 10.62万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18380065/
关键词：
Arabidopsis Nutrient storage Sugar-signal response Transcription factor Seed oil storage Seed maturation program Seed storaze protein

项目摘要

1.Targets and function of ASML1/WRI1 involved in seed oil accumulation.An AP2-type transcriptional activator ASML1/WRI1 of Arabidopsis thaliana is expressed during seed maturation phase. We found that many genes involved in fatty acid synthesis in plastids are direct targets of ASML1/WRI1 and identified consensus DNA binding sequence. While ASML1/WRI1 fascillitates flow of carbon to oil synthesis during seed development, two other ASML1/WRI1 subfamily members are likely to regulate fatty acid synthesis in vegetative tissues2.Function of ASML2 with the CCT domainAlthough we found that CCT domain of ASML2 interacts with B3 domain of ABI3 that controls seed maturation, gene for ASML2 was expressed only weakly during seed maturation phase. Instead, ASML2-Like I that also activates sugar-inducible gene and interacts with ABI3 and HSI2 is expressed during seed maturation phase. We identified a mutant with disruption of ASML2-L1 gene.3. Repression of seed maturation program by HSI2 and HSL1HSI2 and HSL1 are novel B3-EAR transcriptional repressors that repress sugar-inducible gene expression. Seedlings with knockouts of both genes (KK mutant) express embryonic and seed maturation genes and stop growth 7 days after germination, suggesting that HSI2/HSL1 are essential for the repression of seed maturation program and transition to vegetative growth after germination. It was suggested that the C-terminal EAR repression motif of HSI2 is not essential for this function. We identified candidates of targets of HSL1 by using hsi2 knockout plants with DEX-inducible RNAi for HSL1 which enabled us to induce KK mutant phenotype by treatment with DEX.

1.ASML1/ wr1参与种子油脂积累的靶点和功能。拟南芥ap2型转录激活因子ASML1/WRI1在种子成熟期表达。我们发现质体中许多参与脂肪酸合成的基因是ASML1/WRI1的直接靶点，并鉴定出一致的DNA结合序列。ASML1/WRI1在种子发育过程中控制碳向油合成的流动，另外两个ASML1/WRI1亚家族成员可能调节营养组织中的脂肪酸合成2。虽然我们发现ASML2的CCT结构域与控制种子成熟的ABI3的B3结构域相互作用，但ASML2基因在种子成熟阶段仅表达弱。相反，ASML2-Like I也激活糖诱导基因并与ABI3和HSI2相互作用，在种子成熟阶段表达。我们发现了一个ASML2-L1基因断裂的突变体。HSI2和HSL1是抑制糖诱导基因表达的新型B3-EAR转录抑制因子。敲除这两个基因（KK突变体）的幼苗表达胚胎和种子成熟基因，并在发芽后7天停止生长，这表明HSI2/HSL1对抑制种子成熟程序和发芽后向营养生长过渡至关重要。这表明HSI2的c端EAR抑制基序对于该功能不是必需的。我们利用带有DEX诱导的HSL1 RNAi的hsi2敲除植株，确定了HSL1的候选靶点，这使我们能够通过DEX处理诱导KK突变表型。