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INVESTIGATION OF REPLICATION MECHANISM AND PATHOGENESIS OF PARAMYXOVIRUS BY USING INFECTIOUS cDNA CLONE

INVESTIGATION OF REPLICATION MECHANISM AND PATHOGENESIS OF PARAMYXOVIRUS BY USING INFECTIOUS cDNA CLONE
利用感染性cDNA克隆研究副粘病毒的复制机制和发病机制
批准号：
04454200
负责人：
ITO Yasuhiko
金额：
$4.29万
依托单位：
MIE UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1992
资助国家：
日本
项目状态：
已结题
起止时间：
1992 至 1993
来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454200/
关键词：
paramyxovirusparainfluenza virusinfectious cDNARNA RepliconHPIV-2発現ベクター

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英文摘要
At first, the nucleotide sequences of the L gene of Paramyxovirus, simian viruses 5 and 41 were determined. Therefore, amino acid sequences of components of replicative complex, NP, P, and L proteins were also determined. Full-length cDNA clones for NP,P, and L protein could be obtained. Next, artificial mini-genomes, Leader sequence-R1-reporter gene-R2-Trailer sequence, were constructed. The mini-genomes were transfected into human parainfluenza type 2 virus or simian virus 41-infected cells. In this system, replication, transcription and RNA editing were occurred. However, the efficiency was not so high. Subsequently, the mini-genomes, cDNA for NP, P, and L proteins were transfected into the cells infected recombinant vaccinia virus containing T7 polymerase. In this new system, high efficiency of transcription, replication and RNA editing were observed. In another experiment, full-length cDNA clone for human parainfluenza virus type 2 was nearly constructed.
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河野 光雄.鶴留 雅人.沖 尚弘.西尾 真智子.駒田 洋.松村 治雄.草川 茂.太田 尚孝.伊藤 康彦: "Sequence determination of the P gene of simian virus 41: Presence of irregular deletions hear the RNA-edting sites of paramyxoviruses." Journal of General Virology.
Mitsuo Kono，Naohiro Nishio，Hiroshi Kusakawa，Naotaka Ito：“猿病毒 41 的 P 基因的序列测定”。副粘病毒。”普通病毒学杂志。
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駒田 洋.草川 茂.Claes Orvell.鶴留 雅人.西尾 真智子.伴戸 久徳.河野 光雄.松村 治雄.Erling Nirrby.伊藤 康彦: "Antigenic diversity of human parainflueuza virus type 1 isolates and their immunological relatiouship with Sevdai virus revealed by using monoclonal antibodies" Journal of General
Hiroshi Komada，Shigeru Orvell，Masato Mashio，Mitsuo Matsumura，Yasuhiko Ito：“通过使用揭示了 1 型副流感病毒的抗原多样性及其免疫学关系。单克隆抗体》综合杂志
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Y.Furuichi et al.: "Some characteristics of an alpha-amylase inhibitor from phaseolus vulgaris (Cultivar great Northern) seeds." Biosci.Biotech.Biochem.57. 147-148 (1993)
Y.Furuichi 等人：“来自菜豆（大北方品种）种子的 α-淀粉酶抑制剂的一些特征。”
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Ogawa, M., Mutsuga, N., Tsurudome, M., Kawano, M., Matsumura, H., Kusagawa, S., Komada, H., Nishio, M., and Ito, Y.: "Nucleotide sequence analysis of the simian virus 41 gene encoding the large (L) protein and construction of a phylogenetic tree for the L
小川，M.，穆津贺，N.，鹤留，M.，河野，M.，松村，H.，草川，S.，驹田，H.，西尾，M.，和伊藤，Y.：“核苷酸序列分析
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