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Micro modification and evaluation of biomaterial surface

生物材料表面微修饰及评价

基本信息

批准号：
04454308
负责人：
CHINZEI Tsuneo
金额：
$ 4.22万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454308/
关键词：
Surface plasmon microscopy Antithrombogenic material Micromachining Protein adsorption

项目摘要

In the first year of this project, we built up the surface plasmon microscope (SPM) and established the preparation method of specimen. The SPM was newly designed involving the computer controlled adjustment mechanism according to the experience of prototypes. This new design increased the system robustness and resulted rapid sample exchange. As to the specimen, silver was evaporated to the glass, initially. However, silver oxidization occurred and the silver membrane was not tightly attached to the glass. Then, multi layred membrane, chromium and gold, was employed to improve tightness. Polymer membrane which was spin coated on the metal layr, had some problems such as its uniformity and foam formation. These problems are fixed up with adjusting the temperature and atmosphere for after bake.In the last year, we have studied on two theme. First, we pursued the real time measurement of protein adsorption onto the surface of bio-compatible material (polyurethane) and metal electrode (chromium-gold). The albumin adsorption on the polyurethane surface indicated two phase in its time course. The initial rapid phase counted its time constant as 7 minute. This rapid phase was followed by the next slow phase, which time constant was 2 hours, approximately. Gammaglobulin firmly adsorbed not only on the polyurethane membrane but on the chromiumgold surface, in spite of inorganic material.Secondary, we evaluated the effect of surface electrostatic field on protein adsorption. The inhibition of protein adsorption was observed under electrostatic field, which was applied initially before the exposure of protein solution. However, after the completion of protein adsorption, the electrostatic field did not influence the thickness of protein layr. The study has been continued on micro fabricated electrode which applied horizontal electrostatic field within the surface. Also, the effect of AC electrostatic field is still in experiment.

在本项目的第一年，我们搭建了表面等离子体显微镜(SPM)，并建立了样品的制备方法。SPM是根据样机的经验，采用计算机控制的调整机构新设计的。这种新的设计增强了系统的健壮性，实现了快速的样品交换。至于标本，最初银被蒸发到玻璃中。然而，银氧化发生了，银膜没有紧密地附着在玻璃上。然后，采用多层膜，铬和金，以提高密封性。旋涂在金属层上的聚合物膜存在着均匀、泡沫化等问题。这些问题是通过调整烘焙后的温度和气氛来解决的。在过去的一年里，我们研究了两个主题。首先，我们对蛋白质在生物相容性材料(聚氨酯)和金属电极(铬金)表面的吸附进行了实时测量。白蛋白在聚氨酯表面的吸附在其时间过程中表现为两相。初始快相的时间常数为7分钟。这一快速阶段之后是下一个缓慢阶段，其时间常数大约为2小时。无论是无机材料还是无机材料，丙种球蛋白不仅能牢固地吸附在聚氨酯膜上，而且还能牢固地吸附在铬金表面。其次，考察了表面静电场对蛋白质吸附的影响。在蛋白质溶液暴露前施加静电场，观察到对蛋白质吸附的抑制作用。但在蛋白质吸附完成后，静电场对蛋白质层厚度没有影响。在表面施加水平静电场的微细加工电极上进行了研究。另外，交流静电场的作用还在实验中。