Regulation of protein and platelet adsorption on artificial materials with electrical field

电场调控人造材料上蛋白质和血小板的吸附

• 批准号: 08457296
• 负责人: CHINZEI Tsuneo
• 金额: $ 4.22万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457296/
• 关键词: protein adsorption; evanescent light; electric field

In this study, the effect of electric field to regulate protein and platelet adsorption on artificial material was investigated. Protein and platelet adsorption was measured with evanescent light quantitatively.1. Protein and platelet adsorptionProtein and platelet adsorption on polyurethane was measured. Comparative assay was performed among albumin (Alb), gammer-globulin (Glb), mixture of them and whole blood (Wbl). In Alb-PBS,multi-layr adsorption was observed in situ, mono-layr remained after washout. In Glb-PBS,mono-layr adsorption was observed and the thickness after washout was almost same as before. In Alb-Glb-PBS,multi-layr in situ, no change after washout. In Wbl, thick adsorption was observed and was decreased partialy after washout.2. Adsorption regulation with electric fieldElectrical potential was applied on polyurethane surface which was spin-coated on gold electrode at thickness of 2 micrometer. Protein adsorption was observed with evanescent light as above. Initially, Electrical potential suppressed albumin adsorption. After adsorption, however, Electrical potential could not remove adsorbed albumin, totally.

本研究探讨了电场对人工材料吸附蛋白质和血小板的调节作用。用消光光法定量测定蛋白质和血小板的吸附。蛋白质和血小板的吸附测定了蛋白质和血小板在聚氨酯上的吸附。比较白蛋白(Alb)、丙种球蛋白(GLb)及其混合物和全血(WbL)。在Alb-PbS中，原位观察到多层吸附，洗涤后仍保持单层吸附。在GLB-PBS中观察到单层吸附，洗涤后的厚度与洗涤前基本相同。在Alb-GLB-PbS中，多层原位沉积，清洗后没有变化。在WBL中观察到较厚的吸附，并在洗涤后部分减少。在2微米厚的金电极上旋转涂覆的聚氨基甲酸乙酯表面施加电场吸附规律。用上述消逝光观察到蛋白质的吸附。最初，电势抑制了白蛋白的吸附。然而，吸附后，电势不能完全去除吸附的白蛋白。

项目成果

Kunihiko Mabuchi: "Application of micromaching for neural activity measurement" Study on fundamental technology of micromachining, micromacine center. 256-264 (1996)

Kunihiko Mabuchi：“微机械加工在神经活动测量中的应用” 微机械加工基础技术研究，微机械中心。

満淵 邦彦, 鎮西 恒雄: "神経活動のマイクロ計測と電極作成に対するマイクロマシンニング技術の応用" マイクロマシンの基礎技術の研究 財団法人マイクロマシンセンター. 256-264 (1996)

Kunihiko Mitsubuchi，Tsuneo Chinzei：“微加工技术在神经活动微测量和电极创建中的应用”微机械中心基础技术研究256-264（1996）。

鎮西 恒雄: "表面プラズモン共鳴による高分子表面の微細計測" 高分子学会医用高分子研究会講演抄録集. 1-4 (1997)

Tsuneo Chinzei：“通过表面等离子体共振对聚合物表面进行精细测量”摘自高分子科学学会医用高分子研究小组 1-4 (1997)。

Tsuneo Chinzei: "Nano-scale measurement of polymer surface with surface plasmon resonance" Lecture on Branch of Biochemical polymer. 1-4 (1997)

Tsuneo Chinzei：“利用表面等离子体共振对聚合物表面进行纳米级测量”生化聚合物分支讲座。

鎮西恒雄: "表面プラズモン共鳴による高分子表面の微細計測" 高分子学会医用高分子研究会講演抄録集. 1-4 (1997)

Tsuneo Chinzei：“通过表面等离子体共振对聚合物表面进行精细测量”摘自高分子科学学会医用高分子研究小组，1-4 (1997)