Basically research for regenerative tissue engineering to form micro-vascular system from single cells

单细胞形成微血管系统的再生组织工程基础研究

• 批准号: 14380390
• 负责人: CHINZEI Tsuneo
• 金额: $ 4.74万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14380390/
• 关键词: tissue engineering; regenerative medicine; cell location control; co-culture; micro-machining; MEMS; 心筋培養

Observing the native organ structures, the following three factors have to be satisfied to develop three-dimensional organic living structures in the in-vitro regenerative process : (a)appropriate arrangement of any specific cells according to the organ function, (b)appropriate generation of the extracellular matrix, and (c)appropriate induction of capillary blood vessels in the regenerated organ structure. Our group is developing a new culturing system, which could realize all these three factors. The cell culturing chip was fabricated on the silicon wafer with microfabrication techniques. Our immediate target is set to the regeneration of the liver lobule. The mixture of endothelial cells and liver cells is to be cultured on the system where cell culture medium is perfused. The perfused flow through the micro holes punched on the culturing chip would induce the generation of the capillary blood vessels among the cultured liver cells. The eventual goal is to demonstrate that our newly developed culturing system is useful in the regenerative medicine research and that micro fluid flow would induce the generation of capillary blood vessels in the stack of the cultured liver cells.

观察天然器官结构，必须满足以下三个因素以在体外再生过程中形成三维有机活体结构：（a）根据器官功能适当排列任何特定细胞，（B）适当产生细胞外基质，和（c）适当诱导再生器官结构中的毛细血管。我们的团队正在开发一种新的培养系统，可以实现所有这三个因素。利用微加工技术在硅片上制作了细胞培养芯片。我们的直接目标是肝小叶的再生。内皮细胞和肝细胞的混合物将在灌注细胞培养基的系统上培养。通过在培养芯片上穿孔的灌注流将诱导培养的肝细胞之间的毛细血管的生成。最终的目标是证明我们新开发的培养系统在再生医学研究中是有用的，并且微流体流动将在培养的肝细胞堆叠中诱导毛细血管的生成。

项目成果

NOVEL CELL CULTURING SYSTEM WITH MICROMACHINED TEMPLATE FOR THER ALIZATION OF A COMPLEX ORGAN

具有微加工模板的新型细胞培养系统，用于复杂器官的实现

• 发表时间: 2005
• 期刊: IFMBE Proceedings Vol. 11
• 作者: K.Takiura