Development of artificial synapse and artificial nerve with microfabrication

微细加工人工突触和人工神经的开发

• 批准号: 08558093
• 负责人: CHINZEI Tsuneo
• 金额: $ 4.61万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08558093/
• 关键词: Artificial synapse; spinal radicles potential; regeneration microelectrode; 人工シプナス; 微小電極

This study aimed to develop the artificial synapse i.e.microelectrodes to interface the spinal and peripheral neural fiber. As spinal radicles electrodes, the prototype device was designed with custom LSI process. As peripheral nerve electrodes, regeneration microelectrodes were fabricated on the polyimid base. The endoscopic approach and identification method was also developed to distinguish radius in the spinal canal.1.Prototype device for spinal radiclesIntegrated device with nerve fiber electrode and differential amplifier was designed. This device was based on custom LSI chip from VDEC.Insulation of SiNx thin membrane and penetrating hole by anisotropic etching were performed in our process facility.2.Endoscopic approach and identification methodThe endoscopic identification method of relation between dorsal root and peripheral nerve was developed. A small diameter catheter with multiple electrode, 0.5cm interval on longitudinal axis, was inserted in epidural space of goats. Spin … More al evoked potential was measured with this catheter on every pair of electrode. The amplitude and phase of potential was changed at the stimulated root. The electrode distinguished with color marking on the catheter, was searched with endoscope in the spinal canal. In this way, the stimulated root was identified with endoscope. The evoked potential of every radicle of the root was investigated with the thin bipolar electrode through working channel of the endoscope.3.Regeneration microelectrodes for peripheral nervesRegeneration electrodes fabricated on polyimide base with multi-channel access hole were newly designed, and were proved with animal experiment. Both sides of electrode made by copper film pattern, 35 micro-meter thickness, was laminated with polyimide film, 50 micro-meter thickness. Via holes were penetrated with excimer laser, and were coated with gold electroplating. Ischiatic nerve of 12 rats were cut down and were implanted these electrodes. Sacrificed after 4 week, the nerve were reconstructed through the electrode hole histologically, and evoked potential was detected with peripheral stimulation. Less

本研究旨在发展人工突触，即连接脊髓和周围神经纤维的微电极。作为脊髓神经根电极的原型装置采用定制的LSI工艺设计。在聚酰亚胺基底上制备了再生微电极作为周围神经电极。建立了内窥镜下识别椎管内半径的方法。1.脊髓神经根原型装置设计了神经纤维电极和差动放大器一体化装置。该装置基于VDEC定制的LSI芯片，在我们的工艺设备中进行了SiNx薄膜的绝缘和各向异性腐蚀穿孔。2.内窥镜方法和识别方法建立了神经后根与周围神经关系的内窥镜识别方法。在山羊硬膜外间隙置入小口径多电极导管，纵轴间距0.5 cm。Spin…用该导管在每对电极上测得更多的诱发电位。在刺激根处，电位的幅值和相位发生改变。用内窥镜在椎管内探查导管上有颜色标记的电极。这样，就可以用内窥镜识别刺激的神经根。通过内窥镜的工作通道，用薄的双极电极检测每根神经根的诱发电位。3.外周神经再生微电极设计了聚酰亚胺基片上多通道通道孔的再生电极，并用动物实验进行了验证。电极两侧由35微米厚的铜膜图案制成，并用50微米厚的聚酰亚胺薄膜叠层。用准分子激光穿透通孔，镀金。将12只大鼠的坐骨神经切断后植入电极。4周后处死动物，通过电极孔组织学重建神经，外周刺激检测诱发电位。较少

项目成果

鎮西美栄子: "脊髄誘発電位でみる内視鏡下クモ膜下ブロックの選択性" Journal of Anesthesia. 11s. 213-213 (1997)

Mieko Chinzai：“基于脊髓诱发电位的内窥镜蛛网膜下腔阻滞的选择性”《麻醉杂志》11s 213-213 (1997)。

Mieko Chinzei: "Selective dorsal root blockade using spinal endoscopy and electroーphysiology." Anesthesiology. 87(3A). A757-A757 (1997)

Mieko Chinzei：“使用脊柱内窥镜和电生理学进行选择性背根阻滞。”87(3A)。

Mieko Chinzei: "Selectivity of endoscopic spinal blockade proved with spinal evoked potential" Journal of anesthesia. 11s. 213 (1997)

Mieko Chinzei：“通过脊髓诱发电位证明内窥镜脊柱阻滞的选择性”麻醉杂志。

Takafumi suzuki: "Interfacing to nerves with the regeneration microelectrode" Japanese journal of medical electronics and biological engineering. 35 sppl. 182 (1997)

Takafumi suzuki：“通过再生微电极连接神经”日本医学电子和生物工程杂志。

鈴木隆文: "人工心臓の制御を目的とした神経再生型電極の開発" 人工臓器. 26sppl. S-154-S-154 (1997)

Takafumi Suzuki：“用于控制人工心脏的神经再生电极的开发”人工器官。26sppl。