Immunohistological study on immnological activity of P.gingivalis LPS -Effects on immne cells in vitro and in vivo-

牙龈卟啉单胞菌LPS免疫活性的免疫组织学研究-体外和体内对免疫细胞的影响-

• 批准号: 04454474
• 负责人: KATO Ihachi
• 金额: $ 4.42万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454474/
• 关键词: P.gingivalis; Periodontal disease associated bacteria; Endotoxin; Alveolar bone resorption; TNF-alpha; B.gingivalis; TNF-alpha; 免疫系細胞

Two experiments were planned to clarify the immunogical activity of Porphyromonas gingivalis (P.g.) LPS.One was for the effects on alveolar bone resorption in vivo and the other was for the production of TNF-alpha by macrophages in vitro. Escherichia coli (E.coli) LPS was used as control. Each LPS was injected into murine gingiva and alveolar bone was observed histopathologically. In E.coli LPS-injected group alveolar bone resorption was found after four-time injection. On the other hand, bone resorption was delayd in P.g. LPS-injected group. Further the production of TNF-alpha by murine peritoneal macrophages stimulated with each LPS was lower in the P.g. LPS-injected group than in the E.coli LPS-injected group. These results suggested that the bone resorption activity of P.g. LPS in vivo is lower than E.coli LPS.The result of the experiment in vitro, that the production of TNF-alpha induced with P.g. LPS is lower than those with E.coli LPS, might supports this speculation.

计划进行两项实验来阐明牙龈卟啉单胞菌 (P.g.) LPS 的免疫活性。一项是体内对牙槽骨吸收的影响，另一项是体外巨噬细胞产生 TNF-α。大肠杆菌（E.coli）LPS用作对照。将每种LPS注射到小鼠牙龈中并进行组织病理学观察。在注射大肠杆菌LPS的组中，注射四次后发现牙槽骨吸收。另一方面，P.g. 中骨吸收延迟。 LPS注射组。此外，用每种 LPS 刺激的鼠腹膜巨噬细胞产生的 TNF-α 在 P.g. 中较低。 LPS注射组优于大肠杆菌LPS注射组。这些结果表明 P.g. 的骨吸收活性。体内LPS低于大肠杆菌LPS。体外实验结果表明，P.g.诱导TNF-α的产生。 LPS 低于大肠杆菌 LPS，可能支持这一推测。

项目成果

Ikeda, Y., Tani, M., Tani, Y., Hara, Y.and Kato, I.: "Histopathological changes of mice periodontal tissue by injections of P.gingivalis LPS and E.coli LPS." J.Japan. Ass. Periodent.Vol.35, 94. (1993)

Ikeda, Y.、Tani, M.、Tani, Y.、Hara, Y. 和 Kato, I.：“注射牙龈卟啉单胞菌 LPS 和大肠杆菌 LPS 后小鼠牙周组织的组织病理学变化。”

池田康男 ほか: "P.gingivalisおよびE.coli由来LPS連続投与によるマウス歯周組織の経時的組織変化" 日本歯周病学会会誌. 35 秋季特別号. 94- (1993)

Yasuo Ikeda 等人：“由于连续施用源自牙龈卟啉单胞菌和大肠杆菌的 LPS 导致小鼠牙周组织发生颞组织变化”，日本牙周病学会杂志 35 秋季特刊。

池田 康男 ほか: "P.gingivalisおよびE.coli由来LPS連続投与によるマウス歯周組織の経時的組織変化" 日本歯周病学会会誌. 35秋季特別号. 94 (1993)

Yasuo Ikeda 等人：“连续施用源自牙龈卟啉单胞菌和大肠杆菌的 LPS 导致小鼠牙周组织的颞组织变化”，日本牙周病学会杂志 35 秋季特刊（1993 年）。