Immunohistological study of immune system associated with periodontal tissue breakdown by endotoxin

与内毒素破坏牙周组织相关的免疫系统的免疫组织学研究

• 批准号: 02454440
• 负责人: KATO Ihachi
• 金额: $ 3.39万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02454440/
• 关键词: Endotoxin; Histopathological study; Node mouse; Bone resorption; Osteoclast; ELISA法; EIA法; 血清抗体

<Purpose> Endotoxin exhibits the toxic and immunological activities, which and associated with the establishment of periodontal disease under the influence of various immune cells. The purpose of this study is to clarify the role of immune cells on the osteoclastic bone resorption and periodontal inflammation induced by endotoxin in immunized (IM), non-immunized (NI) mice and congenital T Lymphocyte deficet (NU) mice.<Methods and Results> Eight-week-old mice of the BALB/c (+/+) and BALB/c (nu/nu) strains were used for this study. Consecutive infections of E. coli endotoxin into gingival tissue of IM, NI and NU mice were performed. Anti-endotoxin titer of the mouse serum was measured by the ELISA method. IM mice showed osteoclastic bone resorption on alveolar bone and severe inflammation of gingival tissue in earlier period tnan NI mice. The slight inflammation was observed in NU mice compared with IM and NI mice, and was not formed osteoclastic bone resorption. Anti-endotoxin titers of NI and NU mice serum gradually increased, finally overtaked IM mice serum.<Conclusions> In this study, we could make it clear that the existence of T cell has a great influence on osteoclast formation and periodontal inflammation.

目的内毒素具有毒性和免疫学活性，在多种免疫细胞的作用下与牙周病的发生有关。本研究旨在探讨免疫细胞在免疫(IM)、未免疫(NI)和先天性T淋巴细胞缺陷(NU)小鼠破骨细胞性骨吸收和内毒素诱导的牙周炎中的作用。方法与结果：选用8周龄BALB/c(+/+)和BALB/c(nu/nu)品系小鼠。将大肠杆菌内毒素连续感染IM、NI和NU小鼠的牙周组织。用ELISA法测定小鼠血清的抗内毒素效价。IM组小鼠牙槽骨表现为破骨细胞性骨吸收，早期TNaNI组小鼠牙周组织炎症严重。与IM和NI小鼠相比，NU小鼠出现轻度炎症，未形成破骨细胞性骨吸收。NI和NU小鼠血清的抗内毒素效价逐渐升高，最终超过IM小鼠血清。结论T细胞的存在对破骨细胞的形成和牙周炎有很大影响。

项目成果

井村 憲二ほか: "内毒素によるマウスの歯周組織破壊と免疫反応に関する実験的研究第1報" 日本歯周病学会会誌. 33秋季特別号. 76 (1991)

Kenji Imura 等人：“关于内毒素诱导的小鼠牙周组织破坏和免疫反应的实验研究的首次报告”，日本牙周病学会杂志 33 秋季特刊（1991）。

Kenji IMURA et al.: "AN EXPERIMENTAL STUDY OF IMMUNE CELLS ON OSTEOCLAST FORMATION AND PERIODONTAL INFLAMATION INDUCED BY ENDOTOXIN" The 9th International Conference on Periodontal Research. (1992)

Kenji IMURA 等人：“免疫细胞对破骨细胞形成和内毒素诱导的牙周炎症的实验研究”第九届国际牙周研究会议。

Kenji IMURA, Yoshitaka HARA, Eiji ICHIMARU, Atsutoshi YOSHIMURA, Ihachi KATO: "An experimental study of immune cells on osteoclast formation and periodontal inflammation induced by endotoxin" The 9th International Conference on Periodontal Research, Abstr

Kenji IMURA、Yoshitaka HARA、Eiji ICHIMARU、Atsutoshi YOSHIMURA、Ihachi KATO：“免疫细胞对破骨细胞形成和内毒素诱导的牙周炎症的实验研究”第九届国际牙周研究会议，摘要

Kenji IMURA, Eiji ICHIMARU, Yoshitaka HARA , Ihachi KATO: "An experimental study on periodontal tissue breakdown and immune response of mice to endotoxin (Part 1)." J. Japan. Ass. Periodont.33. 76 (1991)

Kenji IMURA、Eiji ICHIMARU、Yoshitaka HARA、Ihachi KATO：“小鼠牙周组织破坏和内毒素免疫反应的实验研究（第 1 部分）”。

井村 憲二 ほか: "内毒素によるマウスの歯周組織破壊と免疫反応に関する実験的研究 第1報" 日本歯周病学会会誌. 33 秋季特別号. 76 (1991)

Kenji Imura 等人：“内毒素诱导的小鼠牙周组织破坏和免疫反应的实验研究，第 1 部分”日本牙周病学会杂志 33 秋季特刊（1991）。