Histopathological study with in situ hybridization on cytokine producing cells in bone ersorption

原位杂交对骨吸收中细胞因子产生细胞的组织病理学研究

• 批准号: 06454544
• 负责人: KATO Ihachi
• 金额: $ 0.64万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454544/
• 关键词: in situ hybridization; IL-1; IFN-gamma; IL-4; bone resorptin; 歯周炎; In situ hybridization; 内毒素

Periodontal disease was generally known as infectious disease caused by periodontopathic bacteria. Immune system, which play an important role on host defense, has been thought to effect on tissue destruction. We had observed that T lymphocyte, especially CD4 positive T lymphocyte, regulates bone resorption observed after repeated injection of Escherechia coli LPS.In this study, using in situ hybridization technique, we tried to clarify the relationship of CD4 positive T lymphocyte subsets, Th1 or Th2, on bone resorption described above. LPS was injected into BALB (c/c) mouse gingiva every 48 hours repeatedly. After 1,4,7 and 10 times injection, mouse were killed and their mandibles were prepared for in situ hybridization technique. Results obtained were as follows. The number of INF-gamma producing cells, as same as IL1-beta, was gradually increased with injection times. On the other hand IL-4 producing cells showed no change. Considering that INF-gamma producing cells are mainly consisted of Th1 lymphocytes, it was suggested that these cells regulate bone resorption with promoting IL1-beta production.

牙周病是由牙周致病菌引起的感染性疾病。免疫系统在机体防御中起着重要作用，被认为对组织的破坏起着重要作用。本研究采用原位杂交技术，探讨了T淋巴细胞，尤其是CD 4阳性T淋巴细胞亚群Th 1、Th 2与骨吸收的关系。BALB（c/c）小鼠牙龈内注射LPS，每48 h重复一次。注射1、4、7、10次后处死小鼠，取下颌骨进行原位杂交。获得的结果如下。与IL 1-β相同，产生INF-γ的细胞的数量随着注射次数逐渐增加。另一方面，IL-4产生细胞没有显示变化。考虑到产生INF-γ的细胞主要由Th 1淋巴细胞组成，提示这些细胞通过促进IL-1-β的产生来调节骨吸收。