Differentiation of small pancreatic mass lesions by the combination of endosonography and genetic analysis of purepancreatic juice

超声内镜与纯胰液基因分析相结合鉴别胰腺小肿块病变

• 批准号: 08670572
• 负责人: OKAI Takashi
• 金额: $ 1.41万
• 依托单位: Cancer Research Institute, Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670572/
• 关键词: endoscopic ultrasonography; K-ras analysis; pure pancreatic juice; pancreatic cancer; inflammatory pancreatic mass; early diagnosis; PCR

Background & Aims : The early diagnosis of pancreatic cancer is still difficult. The purpose of this prospective study was to assess the utility of a combination of endosonography and genetic analysis of pure pancreatic juice for the early recognition of pancreatic cancer. Methods : One hundred and seventy-six patients with syspected pancreatic injury were enrolled and underwent endosonography. Pure pancreatic juice was collected endoscopically in patients with solid pancreatic masses. K-ras point mutations at codon 12 in the juice were assayd by polymerase chain reaction-restriction fragment length polymorphism. Results : Thirty-six patients (20%) were found to have solid pancreatic masses. They consisted of 19 patients with pancreatic cancer (7 patients, <less than or equal> 2cm) and 17 patients with an inflammatory pancreatic mass (13 patients, <less than or equal> 2cm). Although endoscopic retrograde cholangiopancreatography showed high accuracy for cancer diagnosis, ultrasonography and computed tomography were less sensitive, particularly in small pancreatic masses, and 65% of them were not disclosed until endosonography. In contrast, endosonography showed high sensitivity (100%) and specificity (92%) even in small masses. Together with K-ras analysis, assayd safely using small samples, the endosonographic diagnosis became more definitive. Conclusions : Both endosonography and K-ras analysis was safely performed. The combination of endosonography and K-ras analysis of pure pancreatic juice may be useful for the early diagnosis of pancre

背景与目的：胰腺癌的早期诊断仍然是一个难题。这项前瞻性研究的目的是评估超声内镜和纯胰液遗传分析相结合对胰腺癌早期识别的实用性。方法：176例胰腺损伤患者均行超声内镜检查。胰腺实性肿块患者经内镜采集纯胰液。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测果汁中K-ras基因第12位密码子的点突变。结果：36例（20%）患者被发现有实性胰腺肿块。他们包括19名胰腺癌患者（7名患者，<less than or equal>2 cm）和17名胰腺炎性肿块患者（13名患者，<less than or equal>2 cm）。虽然内镜逆行胰胆管造影显示出较高的准确性，癌症的诊断，超声和计算机断层扫描的敏感性较低，特别是在小胰腺肿块，和65%的人没有发现，直到内镜超声。相比之下，超声内镜显示高敏感性（100%）和特异性（92%），即使在小肿块。与K-ras分析一起，使用小样本安全地进行检测，超声内镜诊断变得更加明确。结论：超声内镜和K-ras分析均安全进行。超声内镜检查结合纯胰液K-ras检测有助于胰腺炎的早期诊断

项目成果

Okai T,Yamaguchi Y,Watanabe H,Sawabu N: "An algorithm for the evaluation of the patients with suspected oancreatic cancer by endoscopic ultrasonography with K-ras analysis of pure pancreatic juice. (in Japanese)" Tan to Sui. 19. 55-60 (1998)

Okai T、Yamaguchi Y、Watanabe H、Sawabu N：“一种通过内窥镜超声检查和纯胰液 K-ras 分析来评估疑似胰腺癌患者的算法。（日语）” Tan to Sui。

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Watanabe H 等人：“通过杂交保护测定检测纯胰液中密码子 12 处的 K-ras 点突变，用于诊断胰腺癌。”

Watanabe H,Miyagi C,Yamaguchi Y,Satomura Y,Ohta H,Motoo Y,Okai T,Yoshimura T,Tsuji Y,Sawabu N: "Detection of K-ras point mutations at codon 12 in pancreatic juice for the diagnosis of pancreatic cancer by hybridization protection assay (HPA) : a simple me

Watanabe H、Miyagi C、Yamaguchi Y、Satomura Y、Ohta H、Motoo Y、Okai T、Yoshimura T、Tsuji Y、Sawabu N：“检测胰液中密码子 12 处的 K-ras 点突变以诊断胰腺癌

岡井 高、ほか: "小膵癌(結節型)診断のアルゴリズム(III)、画像検査、膵液K-ras遺伝子" 胆と膵. 19. 55-60 (1998)

Takashi Okai 等人：“小胰腺癌（结节型）的诊断算法（III）、图像检查和胰液 K-ras 基因” Bile and Pancreas 19. 55-60 (1998)。

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Sawabu N 等人：“小胰腺癌病例的临床评估及其早期诊断方法。”