A new combination of endoscopic ultrasonography and DNA diagnosis using pancreatic juice for the early detection of pancreatic cancer

内窥镜超声检查和胰液 DNA 诊断的新组合用于早期发现胰腺癌

基本信息

  • 批准号:
    06670530
  • 负责人:
  • 金额:
    $ 1.22万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
  • 财政年份:
    1994
  • 资助国家:
    日本
  • 起止时间:
    1994 至 1995
  • 项目状态:
    已结题

项目摘要

The combination of endoscopic ultrasonography and DNA diagnosis using pancreatic juice for the detection of small pancreatic cancer was evaluated. The patients with upper abdominal pain. abnormal laboratory data or images on the pancreas, or worsening of diabetic control were enrolled in this study. When the findings of a small pancreatic mass or dilated pancreatic duct were detected by endoscopic ultrasonography, the pure pancreatic juice was collected under a duodenofiberscope for the analyzes of k-ras point mutation at codon 12. The pancreatic cancers less than 2 cm in a diameter were clearly visualized as round hypoechoic tumors with irregular margin. The pancreatic ducts were abruptly dilated caudal to the tumors. Although inflammatory pancreatic masses were generally visualized as square-shaped hypoechoic area without compression or encasement of the neighboring pancreatic ducts, the differentiation from small pancreatic cancer was not always complete, particularly when the tumor was round and the size was less than 1 cm. The incidence of k-ras point mutation analyzed by PCR-RFLP method was 78% (29/37) in patients with pancreatic cancer and 9% (5/53) in those with chronic pancreatitis. The correlation between the incidence of k-ras mutation and tumor size/location were not seen. These results suggest that the combination of endoscopic ultrasonography and DNA diagnosis using pancreatic juice is useful and promising to the early detection of pancreatic cancer.
目的探讨超声内镜联合DNA诊断小胰腺癌的价值。上腹部疼痛的患者。本研究纳入了胰腺的异常实验室数据或图像,或糖尿病控制恶化。当超声内镜检查发现胰腺小肿块或胰管扩张时,在纤维十二指肠镜下收集纯胰液进行k-ras基因第12位密码子点突变分析。直径小于2cm的胰腺癌表现为边缘不规则的圆形低回声肿瘤。胰管在肿瘤尾部突然扩张。虽然胰腺炎性肿块通常表现为方形低回声区,不压迫或包裹邻近的胰管,但与小胰腺癌的鉴别并不总是完全的,特别是当肿瘤是圆形的且大小小于1cm时。PCR-RFLP法检测胰腺癌患者K-ras基因点突变率为78%(29/37),慢性胰腺炎患者为9%(5/53)。K-ras基因突变率与肿瘤大小、部位无关。提示超声内镜结合胰液DNA诊断对胰腺癌的早期诊断具有重要意义。

项目成果

期刊论文数量(38)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Watanabe H,et al:"Detection of K-ras point mutations at codon 12 in pancreatic juice for the diagnosis of pancreatic cancer by hybridization protection assay (HPA): a simple method for the determination of the types of point mutations.21GC02:Jap J Cancer
Watanabe H 等人:“通过杂交保护测定 (HPA) 检测胰液中密码子 12 处的 K-ras 点突变用于诊断胰腺癌:一种确定点突变类型的简单方法。21GC02:Jap
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    0
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Watanabe H,et al:"Detection of K-ras point mutations at codon 12 in pure pancreatic juice for the diagnosis of pancreatic cancer by PCR-RFLP analysis." Pancreas. 12. 18-24 (1996)
Watanabe H 等人:“通过 PCR-RFLP 分析检测纯胰液中密码子 12 处的 K-ras 点突变,用于诊断胰腺癌。”
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    0
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  • 通讯作者:
山口泰志、他:"膵癌患者膵液中p53癌抑制遺伝子変異の検討." 腫瘍マーカー研究会雑誌. 10. 123-124 (1995)
Yasushi Yamaguchi 等人:“胰腺癌患者胰液中 p53 肿瘤抑制基因突变的检查。肿瘤标志物研究组杂志”10. 123-124 (1995)。
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    0
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岡井 高,他:"小膵癌の超音波内視鏡診断" 消化器科.
Takashi Okai 等:《小胰腺癌的超声内镜诊断》消化内科。
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    0
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渡辺弘之、他:"小膵癌診断における膵液中腫瘍マーカーの測定とk-ras点突然変異の検索." 消化器内視鏡. 7. 1137-1146 (1995)
Hiroyuki Watanabe 等人:“胰液中肿瘤标志物的测量和 k-ras 点突变的诊断在胃肠道内窥镜检查中的应用”(1995 年)。
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    0
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OKAI Takashi其他文献

OKAI Takashi的其他文献

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{{ truncateString('OKAI Takashi', 18)}}的其他基金

A basic study of application of high-intensity focused ultrasound for fetal therapy
高强度聚焦超声在胎儿治疗中应用的基础研究
  • 批准号:
    18200035
  • 财政年份:
    2006
  • 资助金额:
    $ 1.22万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Development of Noninvasive Therapeutic Devices and Clinical Techniques for Treating Uterine Fibroid by Occluding Feeding Vessels Through Exposure to High Intensity Focused Ultrasound
通过暴露于高强度聚焦超声来闭塞喂养血管治疗子宫肌瘤的无创治疗装置和临床技术的开发
  • 批准号:
    15200042
  • 财政年份:
    2003
  • 资助金额:
    $ 1.22万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Differentiation of small pancreatic mass lesions by the combination of endosonography and genetic analysis of purepancreatic juice
超声内镜与纯胰液基因分析相结合鉴别胰腺小肿块病变
  • 批准号:
    08670572
  • 财政年份:
    1996
  • 资助金额:
    $ 1.22万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
A study on pathophysiology of circulatory dysfunction in immature fetuses by extrauterine fetal incubation
宫外孵化未成熟胎儿循环功能障碍的病理生理学研究
  • 批准号:
    05454344
  • 财政年份:
    1993
  • 资助金额:
    $ 1.22万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Development and clinical trial of a system for three-dimensional ultrasound
三维超声系统的开发及临床试验
  • 批准号:
    03454497
  • 财政年份:
    1991
  • 资助金额:
    $ 1.22万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Relationship between gastric carcinoma and intestinal metaplasia from the aspect of the expression of placental alkaline phosphatase messenger RNA
从胎盘碱性磷酸酶信使RNA表达角度探讨胃癌与肠化生的关系
  • 批准号:
    02670301
  • 财政年份:
    1990
  • 资助金额:
    $ 1.22万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Development of Extrauterine Fetal Incubation System
宫外胎儿孵化系统的研制
  • 批准号:
    02557063
  • 财政年份:
    1990
  • 资助金额:
    $ 1.22万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research (B)
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