DRUG REGULATIONS OF BONE RESORPTION IN ALVEOLAR BONE AND ITS MECHANISM OF ACTION

牙槽骨骨吸收的药物法规及其作用机制

• 批准号: 05454499
• 负责人: OHYA Keiichi
• 金额: $ 3.97万
• 依托单位: TOKYO MEDICAL AND DENTAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454499/
• 关键词: BONE; OSTEOCLAST; GENE EXPRESSION; IN SITU HYBRIDIZATION; CYTOSKELETON; GOLGI-LYSOSOMAL SYSTEM; CONFOCAL LASER SCANNING MICROSCOPY; BONE RESORPTION; golgi-lysosome系システム; 共焦点レーザースキャン顕微鏡

The following research was performed in order to study the drug regulation of bone resorption occurred in alveolar bone and its mechanism of action. Gene expressions of bone matrix proteins in rats fed with a low calcium diet was studied using a in situ hybridization. In situ hybridization showed the high levels of collagen, osteopontin, osteocalcin and bone sialprotein was located in the osteoblast in rats fed with a low calcium diet compared with the control rats. This observation indicated that bone formation activity was elevated during the low calcium state. Sodium salicylate, a non-steroidal antiinflammatory drug, inhibited the differentiation and the recruitment of osteoclast-like cells in mouse bone marrow culture. This indicates a mechanism of its inhibitory action on bone resorption. Osteoclasts isolated from mouse bone marrow were stained with a fluorescent dyes for Golgi apparatus, ER,and lysosome and were observed with time under the confocal laser scanning microscopy. The changes in cytoplasmic organella were recorded in the living osteoclasts. The present results demonstrates the drug regulations of bone resorption occurred in alveolar bone and its mechanism of action. These research approaches could develop new drugs for preventing alveolar bone resorption in future.

本研究旨在探讨药物对牙槽骨骨吸收的调节作用及其作用机制。应用原位杂交技术研究了低钙饮食大鼠骨基质蛋白基因的表达。原位杂交结果显示，低钙饮食组大鼠成骨细胞中胶原、骨桥蛋白、骨钙素和骨唾液酸蛋白的表达均高于对照组。这一观察结果表明，在低钙状态下骨形成活性升高。水杨酸钠，一种非甾体类抗炎药，在小鼠骨髓培养中抑制破骨细胞样细胞的分化和募集。这表明其对骨吸收的抑制作用的机制。从小鼠骨髓中分离的破骨细胞用高尔基体、ER和溶酶体的荧光染料染色，并在共聚焦激光扫描显微镜下随时间观察。在活的破骨细胞中记录胞质细胞器的变化。本研究结果表明，药物对牙槽骨骨吸收的调节作用及其作用机制。这些研究方法为将来开发预防牙槽骨吸收的新药提供了可能。

项目成果

Soekanto A: "The effect of sodium Salicylate on osteoclast-like cell formation and bone resorptron in a mouse boue marrow culture." Calcified Tissue International. 54. 290-295 (1994)

Soekanto A：“水杨酸钠对小鼠布埃骨髓培养物中破骨细胞样细胞形成和骨吸收管的影响。”

Arai N: "Osteopontin mRNA expression during bone nesorption:an in situ hybridization study of induced ectopic bone in the rat." Bone and Mineral. 22. 129-145 (1993)

Arai N：“骨吸收过程中骨桥蛋白 mRNA 的表达：诱导大鼠异位骨的原位杂交研究。”

SOEKANTO A,OHYA K,OGURA H: "THE EFFECT OF SODIUM SALICYLATE ON OSTEOCLAST-LIKE CELL FORMATION AND BONE RESORPTION IN A MOUSE BONE MARROW CULTURE." CALCIFED TISSUE INTERNATIONAL. 54. 290-295 (1994)

SOEKANTO A、OHYA K、OGURA H：“水杨酸钠对小鼠骨髓培养中破骨细胞样细胞形成和骨吸收的影响。”

Soekanto A: "The effect of sodium salicylate on osteolast-like cell formation and bone resorption in a mouse bone culture." Calcified Tissue International. 54. 290-295 (1994)

Soekanto A：“水杨酸钠对小鼠骨培养物中成骨细胞样细胞形成和骨吸收的影响。”

Ohya K: "Rats fed with a low-calcium diet as an in vivo experimental model for bone resorption." In Pharmacological Approuch to the study of the formation and the resorption mechanism of Hard Tissue. 93-114 (1994)

Ohya K：“用低钙饮食喂养的大鼠作为骨吸收的体内实验模型。”