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Analysis of circadian rhythms of optic lobe visual interneurons in the cricket.

蟋蟀视叶视觉中间神经元的昼夜节律分析。

基本信息

批准号：
05640770
负责人：
TOMIOKA Kenji
金额：
$ 1.34万
依托单位：
YAMAGUCHI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05640770/
关键词：
Circadian rhythm Circadian clock Visual system Insect Neuron Optic lobe 視覚性介在ニューロン

项目摘要

The main purpose of this study was to clarify the circadian rhythm of the optic lobe visual interneurons and its neural basis in the cricket Gryllus bimaculatus. During past two years of this research project, we have got the following new findings.1. We have identified several visual interneurons in the optic lobe suitable for circadian rhythm research by intracellular recording and staining with fluorescent dye. They are the medulla giant interneurons with their somata near the basal medulla, their axons runs toward the contralateral medulla through the brain, and innervate the almost whole area of the contralateral medulla. They were classified into 6 groups according to their receptive filed in the ipsilateral compound eye.2. We have characterized the ciradian rhythm in some of the medulla giant interneurons. Their light induced response was fluctuate in a circadian manner, peaking during the subjective night, while the peaks of the spontaneous activity differed according to the neuron types : Some has peaks during the subjective night while others has during the subjective day.3. HPLC-ECD analysis revealed that serotonin content in the optic lobe fluctuates in circadian manner, peaking in the night. The application of exogenous serotonin reduced the sensitivity of the visual interneurons.These data suggest that the visual system is profoundly regulated by the circadian pacemaker and that serotonin is a possible neurotransmitter involved in the regulation of the sensitivity of the visual interneurons.

本研究的主要目的是阐明蟋蟀视叶视觉中间神经元的昼夜节律及其神经基础。在过去两年的研究中，我们得到了以下新的发现.我们通过细胞内记录和荧光染料染色，确定了视叶中几个适合昼夜节律研究的视觉中间神经元。它们是延髓巨大的中间神经元，其胞体位于基底延髓附近，其轴突穿过大脑向对侧延髓延伸，并支配对侧延髓的几乎整个区域。根据同侧复眼感受野的大小，将其分为6组.我们的特点是ciradian节奏在一些延髓巨大的中间神经元。它们的光诱发反应呈昼夜节律性波动，峰值出现在主观夜间，而自发活动的峰值因神经元类型而异：有的在主观夜间出现峰值，有的在主观白天出现峰值. HPLC-ECD分析显示，5-羟色胺含量在视叶的昼夜波动的方式，在夜间达到高峰。外源性5-羟色胺的应用降低了视觉中间神经元的敏感性，这些数据表明，视觉系统是深刻的昼夜节律起搏器的调节，5-羟色胺是一种可能的神经递质参与调节的视觉中间神经元的敏感性。