MUTUAL INTERACTION BETWEEN RIGHT AND LEFT CEREBRAL LOBES IN INSECTS : COUPLING MECHANISM BETWEEN THE OPTIC LOBE CIRCADIAN CLOCKS

昆虫左右脑叶之间的相互作用:视叶昼夜节律之间的耦合机制

• 批准号: 11168219
• 负责人: TOMIOKA Kenji
• 金额: $ 12.86万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas (A)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11168219/
• 关键词: circadian clock; circadian rhythm; mutual coupling between clocks; serotonin; pigment-dispersing factor (PDF); insect; optic lobe; nervous system; 色素拡散ホルモン用ペプチドPDF; 概日リズム; 視髄両側性ニューロン; フタホシコオロギ; 時計間同調; 蛋白合成

The mechanism of coupling between the two optic lobe circadian clocks was investigated with special reference to the role of serotonin and pigment-dispersing factor (PDF). Light-induced electrical activities of medulla bilateral neuron (MBNs) that convey coupling information showed daily variations, being more intense during the night than the day. Microinjection of serotonin into the optic lobe caused a dose- and time-dependent inhibition of spontaneous and light-induced responses, mimicking the day state. Application of metergoline, a non-selective serotonin antagonist, increased spontaneous activity and light-evoked responses, with higher effect during the day. Further pharmacological investigation revealed that this serotonergic inhibition was mediated by 5-HT_7 like receptors. We then purified the PDF from the cricket brain and made an antibody by immunizing the rabbit. The antibody labeled two groups of neurons in the optic lobe, one located near the lamina and the other near proximal medulla. PDF injected into the optic lobe enhanced the MBN's photo-responsiveness during the day, while little change was induced during the night. The enhancing effect of PDF during the day was suppressed by serotonin. PDF also induced the phase shifts of the locomotor rhythm in a phase dependent manner : it induces phase advance during the late subjective day to early subjective night and delays during the late subjective night to early subjective day. These facts suggest that in the cricket's optic lobe serotonin is released during the daytime and sets the day state in the MBNs, PDF is released during the late day to night and enhances MBNs' photo-responsiveness to set their night state. The dual regulation of MBNs activity and the clock phase by serotonin and PDF may play central role in the stable coupling between the two optic lobe pacemakers.

研究了两个视叶生物钟之间的耦合机制，特别是5-羟色胺和色素分散因子（PDF）的作用。传递耦合信息的延髓双侧神经元（MBNs）的光诱导电活动呈现出昼夜变化，夜间比白天更强烈。将5-羟色胺微量注射到视叶中引起自发性和光诱导反应的剂量和时间依赖性抑制，模仿白天状态。应用非选择性5-羟色胺拮抗剂甲麦角林可增加自发活动和光诱发反应，白天效果更好。进一步的药理学研究表明，这种对肾上腺素能的抑制作用是由5-HT_7样受体介导的。然后，我们从蟋蟀脑中纯化PDF，并通过免疫兔子制备抗体。该抗体标记了两组神经元在视叶，一个位于附近的板和近端髓质。PDF注入到视叶增强MBN的光反应在白天，而在夜间引起的变化不大。白天PDF的增强作用被5-羟色胺抑制。PDF还以相位依赖的方式诱导运动节律的相移：它诱导在主观白天晚期到主观夜晚早期的相位提前和在主观夜晚晚期到主观白天早期的延迟。这些事实表明，在蟋蟀的视叶中，血清素在白天释放并在MBN中设置白天状态，PDF在深夜释放并增强MBN的光响应性以设置其夜间状态。5-羟色胺和PDF对MBNs活动和时钟相位的双重调节可能在两个视叶起搏器之间的稳定耦合中起重要作用。

项目成果

Saifullah ASM, Tomioka K.: "5-Ht-like receptors mediate serotonergic modulation of photo-responsiveness of the medulla bilateral neurons in the cricket, Gryllus bimaculatus"Zoological Science. 20. 303-309 (2003)

Saifullah ASM，Tomioka K.：“5-Ht 样受体介导蟋蟀 Gryllus bimaculatus 髓质双侧神经元光响应性的血清素调节”《动物学科学》。

Nishinokubi I, Tomioka K.: "Analysis of the mechanism underlying the rhythm reversal from diurnal to noctumal in the cricket Gryllus bimaculatus, with special reference to the role of serotonin"Zoological Science. 17. 1075-1080 (2000)

Nishinokubi I，Tomioka K.：“分析蟋蟀双斑蟋蟀从昼夜节律逆转的机制，特别提到血清素的作用”《动物学科学》。

Saifullah, ASM, Tomioka, K.: "5-HT_7-like receptors mediate serotonergic modulation of photo-responsiveness of the medulla bilateral neurons in the cricket, Gryllus bimaculatus"Zoological Science. 20. 303-309 (2003)

Saifullah，ASM，Tomioka，K.：“5-HT_7 样受体介导蟋蟀（Gryllus bimaculatus）髓质双侧神经元光响应性的血清素调节”《动物学科学》。

Saifullah ASM, Tomioka K.: "Serotonin sets the day state in the neurons that control coupling between the optic lobe circadian pacemakers in the cricket Gryllus bimaculatus"Journal of Experimental Biology. 205. 1305-1314 (2002)

Saifullah ASM，Tomioka K.：“血清素在控制蟋蟀 Gryllus bimaculatus 视叶昼夜节律起搏器之间耦合的神经元中设定白天状态”《实验生物学杂志》。

Tomioka, K., Yoshii, T., Saifullah, ASM: "Multioscillator systems controlling the circadian locomotor rhythm in insects. In : Circadian Clock as Multi-Oscillator System (ed by K.Honma and S.Honma)"Hokkaido University Press. 61-74 (2003)

Tomioka, K.、Yoshii, T.、Saifullah, ASM：“控制昆虫昼夜节律的多振荡器系统。见：作为多振荡器系统的昼夜节律时钟（由 K.Honma 和 S.Honma 编辑）”北海道大学出版社。