IDENTIFICATION AND PHYSIOLOGICAL ANALYSIS OF MEDULLA BILATERAL LARGE NEURONS INVOLVED IN MUTUAL INTERACTION OF BILATERALLY PAIRED CIRCADIAN CLOCKS IN INSECTS
IDENTIFICATION AND PHYSIOLOGICAL ANALYSIS OF MEDULLA BILATERAL LARGE NEURONS INVOLVED IN MUTUAL INTERACTION OF BILATERALLY PAIRED CIRCADIAN CLOCKS IN INSECTS
批准号:
08640866
负责人:
TOMIOKA Kenji
金额:
$1.41万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
1996
资助国家:
日本
项目状态:
已结题
起止时间:
1996 至 1997
中文摘要
髓质双侧大神经元(MBNs)是连接双侧生物钟位点的视觉中间神经元,即髓质视神经区。每侧各有26个mbn,根据其形态和感受野可分为4组。在这项研究中,我们研究了四组mbn中哪一组传递时钟之间相互耦合所必需的时钟信息,以及这些神经元如何编码时钟信息。通过高效液相色谱- ecd,我们首先检测了与mbn有关的生物胺。在包括MBN体在内的小组织中,检测到血清素、多巴胺、章鱼胺和一些未知胺。由于MBNs未被抗血清素和抗多巴胺抗体标记,因此它们的神经递质可能是章鱼胺或未知胺。然而,5 -羟色胺和多巴胺系统可能在MBN系统的信息传递中发挥重要作用,因为MBN的电刺激降低了对侧视叶中这些胺的水平。复眼与mbn的接受野相对应的部分破坏表明,mbn -4的接受野位于眼睛的背尾区,最有可能是神经元介导时钟耦合所需的时钟信息的候选人。它们对光的反应比其他mbn要弱得多,但它们的昼夜差异可以清楚地看到,这表明它们将时钟信息编码为它们反应的大小。其他mbn的反应也表现出昼夜差异,尽管他们的反应模式和昼夜变化有所不同。这些结果表明,MBN-4将其一侧的照明信息传递给对侧时钟,并且其他mbn在蟋蟀昼夜节律系统中也具有时钟相关功能。
英文摘要
Medulla bilateral large neurons (MBNs) are visual interneurons connecting the bilateral circadian clock sites, i.e., optic medulla regions. There are 26 MBNs in each side and they could be classified into 4 groups according to their morpholoy and receptive fields. In this study, we examined which of the 4 group of MBNs transmitts the clock information necessary for mutual coupling between the clocks and how those neurons encode the clock information. With HPLC-ECD we first examined the biogenic amines which would be involved in the MBNs. In the small tissue including MBN somata, serotonin, dopamine, octopamine and some unknown amines were detected. Since MBNs were not labelled by anti-serotonin andanti-doapmine antibodies, their neurotransmitter may be either octopamine or the unknown amine. Howeer, serotonergic and dopaminergic system may play an important role in the information transmission in MBN system, since the electricl stimulation of MBNs reduces the level of these amines in the contralateral optic lobe.Partial destruction of the compound eye corresponding to the receptive fields of the MBNs revealed that MBN-4s, whose receptive field lies in the dorso-caudal area of the eye, are the most likely candidates for the neuron mediating the clock information necessary for the clock coupling. Their response to light was rather weaker than those other MBNs, but their day-night difference was clearly seen, suggesting that they encode the clock information as the magnitude of their response. Other MBNs also showed day night difference in their responsiveness, although the pattern of their responses and day night change were different with each other. These results suggest that MBN-4 conveys the lighting information on its side to the contralateral clock and that other MBNs also have clock related functions in the cricket circadian system.
期刊论文(27)
专著(0)
科研奖励(0)
会议论文
登录
查看更多内容
Tomioka K, Yukizane M: "A specific area of the compound eye in the cricket Gryllus bimaculatus sends photic information to the circadian pacemaker in the contralateral optic lobe." J.Comp.Physiol.A. 180. 63-70 (1997)
Tomioka K、Yukizane M:“蟋蟀双斑蟋蟀复眼的特定区域向对侧视叶的昼夜节律起搏器发送光信息。”
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
Tomioka K, Yukizane M: "A specific area of teh compound eye in the cricket Gryllus bimaculatus sends photic information to the circadian pacemaker in the contralateral optic lobe." J.Comp.Physiol.A. 180. 63-70 (1997)
Tomioka K、Yukizane M:“蟋蟀双斑蟋蟀复眼的一个特定区域向对侧视叶的昼夜节律起搏器发送光信息。”
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
Tomioka K: "Neural mechanisms for mutual imteractions between optic lobe pacemakers in the cricker.In:Circadian organization and Oscillatory Coupling.(Honma K & Honma S eds)," Hokkaido University Press, 159-172 (1996)
Tomioka K:“蟋蟀中视叶起搏器之间相互影响的神经机制。见:昼夜节律组织和振荡耦合。(Honma K)
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
Tomioka K, Uwozumi K, Matsumoto N: "Light cycles given during development affect freerunning period of circadian locomotor rthythm of period mutant in Drosophila melanogaster." J.Insect Physiol.43. 297-305 (1997)
Tomioka K、Uwozumi K、Matsumoto N:“发育过程中给予的光周期会影响果蝇周期突变体的昼夜节律运动节律的自由运行期。”
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
Abe Y, Ushirogawa H, Tomioka K: "Circadian locomotor rhythm of the cricket Gryllodes sigillatus. I. Localization of the pacemaker and the photoreceptor." Zool.Sci.14. 719-727 (1997)
Abe Y、Ushirokawa H、Tomioka K:“蟋蟀 Gryllodes sigillatus 的昼夜节律。I. 起搏器和光感受器的定位。”
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
共 21 条
Molecular oscillatory mechanism of the circadian clock in a hemimetabolous insect
-
批准号:15H04400
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$11.65万
-
财政年份:2015
-
负责人:TOMIOKA Kenji
-
依托单位:
Development of a method for evaluation of ageing in cerebral neuronal circuit using circadian rhythm as a gauge
-
批准号:23657056
-
项目类别:Grant-in-Aid for Challenging Exploratory Research
-
资助金额:$2.41万
-
财政年份:2011
-
负责人:TOMIOKA Kenji
-
依托单位:
Analysis of photic entrainment mchanisms of hemimetabolous insects
-
批准号:23370033
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$11.73万
-
财政年份:2011
-
负责人:TOMIOKA Kenji
-
依托单位:
Analysis of oscillatory mechanism of the circadian clock and its function in hemimetabolous insects
-
批准号:19370029
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$8.57万
-
财政年份:2007
-
负责人:TOMIOKA Kenji
-
依托单位:
Analysis of temperature-sensitive oscillatory system in Drosophila circadian clock mechanism
-
批准号:13440250
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$7.23万
-
财政年份:2001
-
负责人:TOMIOKA Kenji
-
依托单位:
MUTUAL INTERACTION BETWEEN RIGHT AND LEFT CEREBRAL LOBES IN INSECTS : COUPLING MECHANISM BETWEEN THE OPTIC LOBE CIRCADIAN CLOCKS
-
批准号:11168219
-
项目类别:Grant-in-Aid for Scientific Research on Priority Areas (A)
-
资助金额:$12.86万
-
财政年份:1999
-
负责人:TOMIOKA Kenji
-
依托单位:
THE MECHANISM OF EXPRESSION OF CIRCADIAN CLOCK RELATED PROTEINS IN AN INSECT OPTIC LOBE
-
批准号:10640667
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$2.3万
-
财政年份:1998
-
负责人:TOMIOKA Kenji
-
依托单位:
DEVELOPMENT OF A METHOD FOR LONG TERM OPTICAL RECORDING OF ELECTRICAL ACTIVITY FROM INSECT NEURONS WITH VOLTAGE SENSITIVE DYES
-
批准号:07554090
-
项目类别:Grant-in-Aid for Scientific Research (A)
-
资助金额:$1.02万
-
财政年份:1995
-
负责人:TOMIOKA Kenji
-
依托单位:
Analysis of circadian rhythms of optic lobe visual interneurons in the cricket.
-
批准号:05640770
-
项目类别:Grant-in-Aid for General Scientific Research (C)
-
资助金额:$1.34万
-
财政年份:1993
-
负责人:TOMIOKA Kenji
-
依托单位:
海外基金