DEVELOPMENT OF A METHOD FOR LONG TERM OPTICAL RECORDING OF ELECTRICAL ACTIVITY FROM INSECT NEURONS WITH VOLTAGE SENSITIVE DYES

开发一种用电压敏感染料长期光学记录昆虫神经元电活动的方法

基本信息

  • 批准号:
    07554090
  • 负责人:
  • 金额:
    $ 1.02万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
  • 财政年份:
    1995
  • 资助国家:
    日本
  • 起止时间:
    1995 至 1997
  • 项目状态:
    已结题

项目摘要

Optical recordings of neural activity are useful for simultaneous analysis of activity of a population of neurons. In this project, we have developed a method which enables us a long term recording of the neural activity with the aid of voltage sensitive dye in an insect central nervous system. We established an effective method for preparation of the insect tissue stained with the dye. We injected RH414, a fluorescence voltage sensitive dye, directly into the nervous tissue. This injection yielded consistent results. The fluorescence was induced through a high intensity halogen light source and detected by a photodiode array camera combined with the Olympus BX30MF fluorescent microscope. The signals were detected and processed by the camera was fed into a photodetector (HR Deltaron, Fuji Film Co.).For automatic long term recording, we developed a computer program which regulates the optical measurements by the camera-photodetector system through GPIB and stores the image data onto a hard disk with a large capacity. Performing this job requires about 8 min. Gains of camera and amplifers, temporal resolution of measurements are preset by the photodetector system. Repetitive trigger pulses from an electronic stimulator drive the series of operation repetitively. It is possible to continue the recording within the capacity of the hard disk.We developed two kind of programs to analyze the huge body of data. One is to calculate the average brightness of each pixel through all the recorded frames, and the other is to count the number of frames which over the threshold brightness of each pixel through all the recorded frames. The calculated data can be visualized by computer as colored images. By these analying method, it is possible to detect temporal changes in electrical activity within rather a wide region of the insect nervous system.
神经活动的光学记录可用于同时分析神经元群体的活动。在这个项目中,我们开发了一种方法,使我们能够在昆虫中枢神经系统中的电压敏感染料的帮助下长期记录神经活动。建立了一种有效的昆虫组织染色的制备方法。我们将荧光电压敏感染料RH 414直接注射到神经组织中。该进样产生了一致的结果。通过高强度卤素光源诱导荧光,并通过与Olympus BX 30 MF荧光显微镜组合的光电二极管阵列相机检测。通过照相机检测和处理信号,并将其送入光电检测器(HR Deltaron,Fuji Film Co.)。为了实现自动长期记录,我们开发了一个计算机程序,该程序通过GPIB调节摄像机-光电探测器系统的光学测量,并将图像数据存储到大容量硬盘上。执行这项工作需要约8分钟。相机和放大器的增益,测量的时间分辨率由光电探测器系统预设。来自电子刺激器的重复触发脉冲重复地驱动一系列操作。我们开发了两种程序来分析大量的数据。一种是通过所有记录的帧计算每个像素的平均亮度,另一种是通过所有记录的帧计数超过每个像素的阈值亮度的帧的数量。计算的数据可以通过计算机可视化为彩色图像。通过这些分析方法,可以在昆虫神经系统的相当广泛的区域内检测电活动的时间变化。

项目成果

期刊论文数量(31)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
富岡憲治: "時間を知る生物" 裳華房(印刷中), (1996)
富冈健二:“懂得时间的生物”Shokabo(正在出版),(1996)
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    0
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富岡憲治: "5・11測時機構.(分担執筆)三橋淳他編:昆虫学-基礎と応用" 朝倉書店(印刷中), (1997)
Kenji Tomioka:“5/11 时间测量机制。(贡献者)Jun Mitsuhashi 等编辑:昆虫学 - 基础知识和应用”朝仓书店(目前正在印刷),(1997 年)
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    0
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  • 通讯作者:
Tomioka K: "Circadian clocks in the cricket (in Japanese)" Kontyu to Shizen. 30. 23-28 (1995)
Tomioka K:“板球运动中的生物钟(日语)” Kontyu to Shizen。
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  • 发表时间:
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    0
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  • 通讯作者:
Murata, T., Matsumoto, A., Tomioka, K., Chiba, Y.: "Ritsu : a clock mutant from natural population of Drosophila melanogaster." Journal of Neurogenetics. 9. 239-249 (1995)
Murata, T.、Matsumoto, A.、Tomioka, K.、Chiba, Y.:“Ritsu:来自黑腹果蝇自然种群的时钟突变体。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Yukizane M. Tomioka K: "Neural pathways involved in mutual interactions between optic lobe circadian pacemakers in the cricket Gryllus bimaculatus." J. Comp. Physiol. A. 176. 701-706 (1995)
Yukizane M. Tomioka K:“蟋蟀 Gryllus bimaculatus 视叶昼夜节律起搏器之间相互作用的神经通路。”
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  • 影响因子:
    0
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TOMIOKA Kenji其他文献

TOMIOKA Kenji的其他文献

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{{ truncateString('TOMIOKA Kenji', 18)}}的其他基金

Molecular oscillatory mechanism of the circadian clock in a hemimetabolous insect
半变态昆虫生物钟的分子振荡机制
  • 批准号:
    15H04400
  • 财政年份:
    2015
  • 资助金额:
    $ 1.02万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of a method for evaluation of ageing in cerebral neuronal circuit using circadian rhythm as a gauge
开发以昼夜节律为衡量标准的脑神经元回路老化评估方法
  • 批准号:
    23657056
  • 财政年份:
    2011
  • 资助金额:
    $ 1.02万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Analysis of photic entrainment mchanisms of hemimetabolous insects
半变态昆虫光夹带机制分析
  • 批准号:
    23370033
  • 财政年份:
    2011
  • 资助金额:
    $ 1.02万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Analysis of oscillatory mechanism of the circadian clock and its function in hemimetabolous insects
半变态昆虫生物钟振荡机制及其功能分析
  • 批准号:
    19370029
  • 财政年份:
    2007
  • 资助金额:
    $ 1.02万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Analysis of temperature-sensitive oscillatory system in Drosophila circadian clock mechanism
果蝇生物钟机制中温度敏感振荡系统的分析
  • 批准号:
    13440250
  • 财政年份:
    2001
  • 资助金额:
    $ 1.02万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
MUTUAL INTERACTION BETWEEN RIGHT AND LEFT CEREBRAL LOBES IN INSECTS : COUPLING MECHANISM BETWEEN THE OPTIC LOBE CIRCADIAN CLOCKS
昆虫左右脑叶之间的相互作用:视叶昼夜节律之间的耦合机制
  • 批准号:
    11168219
  • 财政年份:
    1999
  • 资助金额:
    $ 1.02万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas (A)
THE MECHANISM OF EXPRESSION OF CIRCADIAN CLOCK RELATED PROTEINS IN AN INSECT OPTIC LOBE
昆虫视神经节律相关蛋白的表达机制
  • 批准号:
    10640667
  • 财政年份:
    1998
  • 资助金额:
    $ 1.02万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
IDENTIFICATION AND PHYSIOLOGICAL ANALYSIS OF MEDULLA BILATERAL LARGE NEURONS INVOLVED IN MUTUAL INTERACTION OF BILATERALLY PAIRED CIRCADIAN CLOCKS IN INSECTS
昆虫中参与双侧生物钟相互作用的延髓双侧大神经元的鉴定和生理分析
  • 批准号:
    08640866
  • 财政年份:
    1996
  • 资助金额:
    $ 1.02万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Analysis of circadian rhythms of optic lobe visual interneurons in the cricket.
蟋蟀视叶视觉中间神经元的昼夜节律分析。
  • 批准号:
    05640770
  • 财政年份:
    1993
  • 资助金额:
    $ 1.02万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

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