In-situ-hybridization analysis of HTLV-1-related diseases, employing highly biotinylated probes synthesized by polymerase chain reaction
In-situ-hybridization analysis of HTLV-1-related diseases, employing highly biotinylated probes synthesized by polymerase chain reaction
批准号:
05670176
负责人:
HASUI Kazuhisa
金额:
$1.41万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for General Scientific Research (C)
财政年份:
1993
资助国家:
日本
项目状态:
已结题
起止时间:
1993 至 1995
中文摘要
本项目旨在利用聚合酶链式反应合成的高度生物素化的探针进行原位杂交(ISH),检测HTLV-1相关疾病,特别是恶性淋巴瘤(MLS)细胞成分中的HTLV-1前病毒DNA及其信号。ISH未能检测到HTLV-1前病毒DNA,但成功地在HTLV-1相关细胞系细胞和人ML细胞的石蜡切片中检测到其信号。HTLV-1前病毒DNA Px Tax区信号在包括成人T细胞白血病/淋巴瘤(ATLL)在内的T细胞多形性淋巴瘤(T-Pleo)中最常见。部分低度T细胞MLs和其他MLs中的信号提示,除ATLL外,还存在HTLV-1相关的MLs,并从HTLV-1相关低度T细胞MLs向ATLL的促进作用。抗原恢复免疫组织化学检测显示,HTLV-1p40Tax在MLS中有信号,但分布在许多T细胞MLL的ML细胞胞浆和少量T细胞MLL的细胞核中,提示HTLV-1前病毒DNA信号与宿主细胞基因激活的直接比较不能看到HTLV-1 p40Tax的反式激活功能。抗原修复和免疫MAX法可见少量的p40Tax分布于ATLL细胞的胞核。然后,考虑到HTLV-1 p40Tax的反式激活以外的功能,采用ISH、抗原修复和免疫MAX相结合的方法,分析HTLV-1相关疾病中HTLV-1前病毒DNA的激活与宿主细胞抗原的关系。
英文摘要
This project aimed to detect HTLV-1 proviral DNA and its signals in cellular composition of HTLV-1-related diseases, especially malignant lymphomas (MLs), by means of in-situ-hybridization (ISH) employing highly biotinylated probes synthesized by polymerase chain reaction. The ISH failed to detect HTLV-1 proviral DNA but succeeded to detect its signals in paraffin sections of HTLV-1-related cell line cells and human ML cells. The signals of HTLV-1 proviral DNA pX Tax region were recognized the most frequently in T-cell pleomorphic lymphoma (T-Pleo) including adult T-cell leukemia/lymphoma (ATLL). The signals in some of low-grade T-cell MLs and the other MLs suggested the existence of HTLV-1-related MLs other than ATLL and the promotion from HTLV-1-related low-grade T-cell MLs to ATLL.Antigen-retrieval immunohistochemical detection of HTLV-1 p40tax was performed in the MLs revealing the signals in ISH.Bur p40tax distributed in cytoplasm of ML cells in many T-cell MLs and in nuclei in a small number of T-cell MLs, suggesting that the direct comparision of the signals of HTLV-1 proviral DNA and the activation of host cell genes would fail to see the transactivating function of HTLV-1 p40tax. A small amount of p40tax in the nuclei of ATLL cells could be visualized by antigen-retrieval and ImmunoMax method. Then, the relation of the activation of HTLV-1 proviral DNA and host cell gens in HTLV-1-related diseases will be able tp be analyzed by means of the combination of the ISH and the antigen-retrieval and ImmunoMax method, considering the HTLV-1 p40tax's functions other than the transactivation.
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Hasui K,Sato E,Sueyoshi K,Kitajima S,Goto M,Nakamura T,Matsumoto D: "Cold in-situ-hybridization of human T-cell leukemia virus type 1 : Production of biotinylated probes by polymerase chain reaction and improvement of procedures." DENDRITIC CELLS. 5. 27-3
Hasui K,Sato E,Sueyoshi K,Kitajima S,Goto M,Nakamura T,Matsumoto D:“人 T 细胞白血病病毒 1 型冷原位杂交:通过聚合酶链反应生产生物素化探针和程序改进
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通讯作者:
Hasui K,Sato E,Iwamoto H et al: "Malignant lymphoma of young people under 20 years of age in an endemic area of huma t-cell leukemia virus type 1" Lymphoreticular cells and disease,Proceeding of the third Korean-Japanese Lymphoreticular workshop. 181-198
Hasui K,Sato E,Iwamoto H等:“人类T细胞白血病病毒1型流行区20岁以下年轻人的恶性淋巴瘤”淋巴网状细胞和疾病,第三届韩日淋巴网状研讨会论文集
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Hasui K,Jia HM,Jia SS,Nomoto M and Sato E: "Antigen retrieval in paraffin-immunohistochemistry detecting monoclonality of immunoglobulin light chain in B-cell malignant lymphomas:Comparison with ISH." Proceeding of the fourth Japanese-Korean lymphoreticul
Hasui K、Jia HM、Jia SS、Nomoto M 和 Sato E:“石蜡免疫组织化学中的抗原修复检测 B 细胞恶性淋巴瘤中免疫球蛋白轻链的单克隆性:与 ISH 的比较。”
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通讯作者:
Hasui K,Sato E et al: "Proceedings of Third Korean-Japanese Lymphoreticular Workshop-1993" Malignant lymphomas of young people under 20 years of age in an endemic area of Human T-cell leukemia virus type1(in press),
Hasui K,Sato E等人:“第三届韩日淋巴网状研讨会论文集-1993”人类T细胞白血病病毒1型流行地区20岁以下年轻人的恶性淋巴瘤(正在出版),
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Hasui K et al: "Malignant lymphomas of young people under 20 years of age in an endemic area of human T-cell leukemia virus type 1. In Lymphoreticular Cells and Diseases" Hematopoietic-Lymphoreticular Study Group, the Korean Society of Pathologists, Seoul
Hasui K 等人:“人类 T 细胞白血病病毒 1 型流行区 20 岁以下年轻人的恶性淋巴瘤。淋巴网状细胞和疾病”,韩国病理学家学会造血-淋巴网研究组,首尔
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共 32 条
Academic project of oncogenic factors in malignant tumors and the related diseases in northeast China
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批准号:19406007
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$10.73万
-
财政年份:2007
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负责人:HASUI Kazuhisa
-
依托单位:
Academic research project on oncogenic factors in malignant tumors in the eastnorthern China
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批准号:16406006
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$6.34万
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财政年份:2004
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负责人:HASUI Kazuhisa
-
依托单位:
Development of preditection method to prevent occurrence of adult T-cell leukemia/lymphoma (ATLL)
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批准号:13557017
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$8.77万
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财政年份:2001
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负责人:HASUI Kazuhisa
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依托单位:
Academic research project on oncogenic factors in malignant lymphomas in the east-northern China
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批准号:13576003
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$5.82万
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财政年份:2001
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负责人:HASUI Kazuhisa
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依托单位:
Oncogenic factors in Chinese gastric lymphoma
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批准号:11691216
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项目类别:Grant-in-Aid for Scientific Research (B).
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资助金额:$2.43万
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财政年份:1999
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负责人:HASUI Kazuhisa
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依托单位:
A STUDY OF HTLV-1-RELATED DISEASES BY MEANS OF HIGHLY SENSITIVE HISTOCHEMISTRY
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批准号:10670166
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.05万
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财政年份:1998
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负责人:HASUI Kazuhisa
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依托单位:
海外基金