Development of preditection method to prevent occurrence of adult T-cell leukemia/lymphoma (ATLL)

本项目进行了以下研究，旨在预测成人T细胞白血病/淋巴瘤（ATLL）的发生。1、制备白血病患者的外周血标本，包括ATLL、HTLV-1携带者和其他淋巴细胞增多症患者。2、建立了超灵敏的免疫组织化学--简化催化信号放大（nsCSA）系统。3.通过使用抗HTLV-1 Tax抗体的nsCSA系统，显示了在包括PBTS的组织标本中的ATLL细胞中表达极少量的Tax。4、用nsCSA系统检测HTLV-1感染细胞的肿瘤特征，Ki 67抗原和p53蛋白的表达。(1)In HTLV-1携带者的粒细胞核被抗Ki-67抗原抗体标记，提示HTLV-1感染对造血的影响。（2）p53 1801抗体的nsCSA系统检测到与凋亡相关的p53表达，因为p53中调节其凋亡功能的Ser 46被p53 1801标记， ...更多信息 tibody。（3）在ATLL、HTLV-1携带者和传染性单核细胞增多症中，p53磷酸化的Ser 392被p53 Phos的nsCSA系统标记，提示p53失活。（4）p53 Phos和p531801抗体在HTLV-1携带者中的表达随年龄增长而增加（Kruskal-Walli检验）。5、通过引入经典的甘氨酸处理和阻断一抗的非特异性反应，建立了自身免疫染色的多重免疫染色方法。6、通过网上的DNA数据库，可以确定检测目的DNA mRNA的寡核苷酸探针的DNA序列。众所周知，杂交溶液和检测系统是mRNA原位杂交的重要因素。在原位PCR方法中，利用载玻片表面温度传感器对载玻片上组织切片的温度进行控制是可能的。下一阶段检测系统预计将引入nsCSA系统（291字）减

This project performed following investigations, aiming to predict occurrence of adult T-cell leukemia/lymphoma (ATLL). 1,Peripheral blood tissue specimens (PBTS) of leukemia including ATLL, HTLV-1 carriers and the other lymphocytosis were prepared. 2,Supersensitive immunohistochemistry, new simplified catalyzed signal amplification (nsCSA) system, was established. 3,By the nsCSA system employing anti-HTLV-1 Tax antibody, it was shown that an extremely small amount of Tax was expressed in ATLL cells in tissue specimens includings PBTS. 4,As neoplastic features of HTLV-1-infected cells, expression of Ki67 antigen and p53 protein was examined by the nsCSA system. (1)In HTLV-1 carriers, segmented nuclei of granulocytes were labeled by anti-Ki67 antigen antibody, suggesting an effect of HTLV-1 infection on hematopoiesis. (2)The nsCSA system of p53 1801 antibody detected expression of p53 related to apoptosis, since Ser46 in p53 regulating its function in apoptosis is labeled by p53 1801 an … More tibody. (3)Phosphorylated Ser392 in p53 was labeled by the nsCSA system of p53Phos in ATLL, HTLV-1 carriers and infectious mononucleosis, suggesting inactivation of p53. (4)Expression of p53Phos and p53 1801 antibody was significantly observed in advam\ncing age of HTLV-1 carriers (Kruskal-Walli test). 5,Multi-immunostaing method by an autoimmunostainer was established by introducing classic glycine treatment and blocking non-specific reaction of primary antibody. 6, By the DNA database on the web, it was possible to determine DNA sequence of oligonucleotide probe detecting mRNA of the target DNA. It was known that hybridization solution and detection system to visualize hybridized oligonucleotide probes were important factors in mRNA in-situ hybridization. By means of a sensor of slide glass surface temperature, it was possible to control temperature of tissue section on the slide glass in in-situ PCR method. The next stage detection system expects introduction of the nsCSA system (291 words) Less