Oncogenic factors in Chinese gastric lymphoma

1、本课题就中国地区幽门螺杆菌(Hp)感染情况、中日两国胃淋巴瘤的临床病理差异、胃淋巴瘤细胞免疫球蛋白重链基因可变区(Ig H V)的研究现状进行了专题报告。2、在中国人胃淋巴瘤中，有许多晚期病例，也有一些巨大的淋巴瘤细胞。3、胃淋巴瘤细胞中CD68和/或胸苷磷酸化酶(TP)阳性树突状细胞提示交织T细胞参与了胃B细胞淋巴瘤的组织发生。对IgH V DNA序列的分析表明，在胃MALT型淋巴瘤的发生中存在细菌的共同感染，与多种人类基因的同源性以及在高度恶性胃淋巴瘤的发生中正在进行的体细胞突变。5、检测全身…中产生具有突变性的一氧化氮(NO)的诱导型一氧化氮合酶(INOS)的表达淋巴器官、幽门螺杆菌相关性胃溃疡和胃淋巴瘤较多。(1)iNOS在生发中心的表达与Hp感染密切相关。(2)幽门螺杆菌体脂多糖Lewis X/Y沉积在区域淋巴结的生发中心。(3)胃MALT型淋巴瘤中iNOS在交织生发中心表达。那么，Hp对粘膜免疫的致病作用和对胃MALT型淋巴瘤的致癌性可能出现在生发中心，在那里iNOS被Lewis X/Y诱导并产生6号，在某些情况下少数带有EBER-1信号的淋巴瘤细胞提示EBV感染是胃淋巴瘤发生发展的因素之一，尽管大多数淋巴瘤细胞都丢失了EBV基因。7、本研究项目发展了利用网络设计寡核苷酸探针检测API2和MALT-1基因的病理状态的方法，其中嵌合基因在NP无关的MALT型淋巴瘤中发现。较少

1, This research project held a symposium to report the prevalence of Helicobacter pyloris (HP) infection in China, no difference in clinicopathology between Chinese and Japanese gastric lymphomas, and the present state of researches of immunoglobulin heavy chain gene variable region (Ig H V) in gastric lymphoma cell.2, In Chinese gastric lymphoma cases exsmined, there were many advanced cases and some cases with giant lymphoma cells.3, CD68- and/or thymidine phosphorylase (TP)-positive dendritic cells among gastric lymphoma cells suggested the participation of intermingling T cell in histogenesis of the gastric B-cell lymphomas.4, IgH V DNA sequences analyzed suggested bacterial co-infection in development of gastric MALT type lymphoma, and homology to various human genes and on-going somatic mutation in development of high-grade gastric lymphomas.5, Expression of Inducible nitric oxide synthase (iNOS), which produces nitric oxide (NO) with mutagenicity, was examined in the Whole-body … More lymph apparatus, HP-related gastric ulcer and gastric lymphomas. (1) The expression of iN0S in germinal centers had a close relation with HP infestation. (2) Lewis X/Y, lypopolysaccharides of HP body, deposits in the germinal center of the regional lymph nodes. (3) iNOS expressed in the intermingling germinal centers in gastric MALT type lymphoma. Then, HP pathogenity to the mucosal immunity and HP carcinogenicity to gastric MALT type lymphoma may appear in the germinal centers, where iNOS was induced by Lewis X/Y and produced NO.6, In some cases a few lymphoma cells with EBER-1 signals suggested that infection of Epstein-Barr virus (EBV) infection is one of progression factors in the oncogenesis of gastric lymphoma, although most of the lymphoma cells lost the EBV genome.7, This research project developed the web-utilized method that designs oligonucleotides probes detecting pathological condition of API2 and MALT-1 genes, of which the chimeric gene was found in NP-non-related MALT type lymphoma. Less