Oncogenic factors in Chinese gastric lymphoma

1、本研究项目召开专题讨论会，报告中国幽门螺杆菌（HP）感染的流行情况，中国和日本胃淋巴瘤的临床病理无差异，以及胃淋巴瘤细胞免疫球蛋白重链基因可变区（IG H V）的研究现状。3、胃淋巴瘤细胞中CD 68和/或胸苷磷酸化酶（TP）阳性树突状细胞提示混合T细胞参与了胃B细胞淋巴瘤的组织发生。IgH V DNA序列分析提示胃MALT型淋巴瘤的发生与细菌共感染有关，与人类多种基因同源，并在胃高级别淋巴瘤的发生中存在持续的体细胞突变。5、检测了诱导型一氧化氮合酶（iNOS）的表达，该酶产生具有致突变性的一氧化氮（NO）。 关于我们 淋巴器、HP相关胃溃疡和胃淋巴瘤。(1)iN 0 S在生殖中心的表达与HP感染密切相关。(2)HP小体脂多糖刘易斯X/Y沉积于区域淋巴结的生发中心。(3)iNOS在胃MALT型淋巴瘤的混合生殖中心表达。HP对粘膜免疫的致病性和HP对胃MALT型淋巴瘤的致癌性可能出现在生殖中心，在那里iNOS被刘易斯X/Y诱导并产生NO.6，在某些情况下，少数淋巴瘤细胞具有EBER-1信号，提示EB病毒（EBV）感染是胃淋巴瘤发生的进展因素之一，尽管大多数淋巴瘤细胞丢失了EBV基因组。7、本研究项目开发了网络利用的方法，设计检测病理状态的API 2和MALT-1基因的寡核苷酸探针，其中嵌合基因在NP-非相关MALT型淋巴瘤中被发现。少

1, This research project held a symposium to report the prevalence of Helicobacter pyloris (HP) infection in China, no difference in clinicopathology between Chinese and Japanese gastric lymphomas, and the present state of researches of immunoglobulin heavy chain gene variable region (Ig H V) in gastric lymphoma cell.2, In Chinese gastric lymphoma cases exsmined, there were many advanced cases and some cases with giant lymphoma cells.3, CD68- and/or thymidine phosphorylase (TP)-positive dendritic cells among gastric lymphoma cells suggested the participation of intermingling T cell in histogenesis of the gastric B-cell lymphomas.4, IgH V DNA sequences analyzed suggested bacterial co-infection in development of gastric MALT type lymphoma, and homology to various human genes and on-going somatic mutation in development of high-grade gastric lymphomas.5, Expression of Inducible nitric oxide synthase (iNOS), which produces nitric oxide (NO) with mutagenicity, was examined in the Whole-body … More lymph apparatus, HP-related gastric ulcer and gastric lymphomas. (1) The expression of iN0S in germinal centers had a close relation with HP infestation. (2) Lewis X/Y, lypopolysaccharides of HP body, deposits in the germinal center of the regional lymph nodes. (3) iNOS expressed in the intermingling germinal centers in gastric MALT type lymphoma. Then, HP pathogenity to the mucosal immunity and HP carcinogenicity to gastric MALT type lymphoma may appear in the germinal centers, where iNOS was induced by Lewis X/Y and produced NO.6, In some cases a few lymphoma cells with EBER-1 signals suggested that infection of Epstein-Barr virus (EBV) infection is one of progression factors in the oncogenesis of gastric lymphoma, although most of the lymphoma cells lost the EBV genome.7, This research project developed the web-utilized method that designs oligonucleotides probes detecting pathological condition of API2 and MALT-1 genes, of which the chimeric gene was found in NP-non-related MALT type lymphoma. Less