Academic research project on oncogenic factors in malignant lymphomas in the east-northern China

本研究采用免疫组化和EBER-1原位杂交技术对我国东北地区鼻腔恶性淋巴瘤的病理标本进行分析，发现EB病毒（EBV）相关的NK细胞淋巴瘤依次为扁桃体、口腔和鼻腔。在这些鼻NK细胞淋巴瘤中，在鼻NK细胞淋巴瘤的背景中，许多巨噬细胞中注意到凋亡产物的受体CD 204的表达，这表明退行性变的趋势是凋亡，尽管未检测到p53蛋白的异常表达和磷酸化p53蛋白的高表达。在下一个项目中，我们检查了切割的caspase-3的免疫组织化学，发现免疫组织化学可以检测到细胞在核变性之前陷入凋亡。在中国人鼻NK细胞淋巴瘤背景中的基质细胞分析中，当在外周T细胞淋巴瘤中看到T细胞依赖性树突状细胞时，在这些淋巴瘤中注意到未成熟的胸苷磷酸化酶阳性基质细胞。在下一个项目中，我们检查了干细胞1 s标记物的免疫组织化学是否可以应用于检测骨髓、外周血和组织中的干细胞，并发现可以通过这种方法标记人类病理标本中的组织干细胞。当本项目显示鼻型NK细胞淋巴瘤在中国罕见时，本项目尚不清楚是否存在鼻型NK细胞淋巴瘤的先兆病变。在本项目期间，准备了本项目的研究伦理方面，因为本项目是一个分析中医病理标本的国际项目。

This research-project analyzed pathology-specimens of nasal malignant lymphomas in the east-northern China by means of immunohistochemistry and EBER-1 in-situ hybridization and found more Epstein-Barr virus (EBV)-related NK-cell lymphomas in the order of tonsil, oral cavity and nose. In these nasal NK-cell lymphomas, expression of CD204, a receptor for apoptosis products, was noted in many macrophages in the background of the nasal NK-cell lymphomas, suggesting that degenerative tendency was that of apoptosis, although abnormal expression of p53 protein and high expression of phosphorylated p53 protein were not detected. For the next project, we checked immunohistochemistry of cleaved caspase-3 and found that the immunohistochemistry can detect cells falling in apoptosis before nuclear degeneration. In analysis of stromal cells in the background Chinese nasal NK-cell lymphomas, immature thymidine phosphorylase-positive stromal cells were noted in these lymphomas, when T-cell-dependent dendritic cells are seen in peripheral T-cell lymphomas. For the next project, we checked whether immunohistochemistry of stem cell 1 s markers can be applied to detect stem cells in the bone marrow, peripheral blood and the tissues and found that it is possible by this method to label tissue stem cells in human pathology-specimens. It was unknown in this project whether there were premonitory lesions of nasal NK-cell lymphomas, when it was shown in this project that nasal type NK-cell lymphomas were rare in China. In the period of this project, research ethical aspects of this project were prepared, since this project is an international one analyzing pathology specimens in the Chinese medicine.