Academic research project on oncogenic factors in malignant lymphomas in the east-northern China

本课题采用免疫组化和EBER-1原位杂交的方法对中国东北地区鼻恶性淋巴瘤病理标本进行分析，发现与eb病毒相关的nk细胞淋巴瘤以扁桃体、口腔、鼻居多。在这些鼻腔nk细胞淋巴瘤中，在鼻腔nk细胞淋巴瘤的背景下，在许多巨噬细胞中发现了细胞凋亡产物受体CD204的表达，提示细胞的退行性倾向是细胞凋亡，尽管未检测到p53蛋白的异常表达和磷酸化p53蛋白的高表达。在接下来的项目中，我们检测了裂解的caspase-3的免疫组化，发现免疫组化可以检测到细胞核变性前细胞凋亡的下降。在对中国鼻nk细胞淋巴瘤基质细胞的分析中，当外周血t细胞淋巴瘤中可见t细胞依赖性树突状细胞时，在这些淋巴瘤中发现未成熟的胸苷磷酸化酶阳性基质细胞。在接下来的项目中，我们检查了干细胞1 s标记物的免疫组织化学是否可以用于检测骨髓、外周血和组织中的干细胞，发现可以用这种方法标记人类病理标本中的组织干细胞。本项目不知道鼻部nk细胞淋巴瘤是否有先兆病变，但本项目显示鼻部nk细胞淋巴瘤在中国较为罕见。在这个项目期间，由于这个项目是一个国际性的中医病理标本分析项目，所以在这个项目的研究伦理方面做了准备。

This research-project analyzed pathology-specimens of nasal malignant lymphomas in the east-northern China by means of immunohistochemistry and EBER-1 in-situ hybridization and found more Epstein-Barr virus (EBV)-related NK-cell lymphomas in the order of tonsil, oral cavity and nose. In these nasal NK-cell lymphomas, expression of CD204, a receptor for apoptosis products, was noted in many macrophages in the background of the nasal NK-cell lymphomas, suggesting that degenerative tendency was that of apoptosis, although abnormal expression of p53 protein and high expression of phosphorylated p53 protein were not detected. For the next project, we checked immunohistochemistry of cleaved caspase-3 and found that the immunohistochemistry can detect cells falling in apoptosis before nuclear degeneration. In analysis of stromal cells in the background Chinese nasal NK-cell lymphomas, immature thymidine phosphorylase-positive stromal cells were noted in these lymphomas, when T-cell-dependent dendritic cells are seen in peripheral T-cell lymphomas. For the next project, we checked whether immunohistochemistry of stem cell 1 s markers can be applied to detect stem cells in the bone marrow, peripheral blood and the tissues and found that it is possible by this method to label tissue stem cells in human pathology-specimens. It was unknown in this project whether there were premonitory lesions of nasal NK-cell lymphomas, when it was shown in this project that nasal type NK-cell lymphomas were rare in China. In the period of this project, research ethical aspects of this project were prepared, since this project is an international one analyzing pathology specimens in the Chinese medicine.