Academic research project on oncogenic factors in malignant lymphomas in the east-northern China

本研究采用免疫组织化学和EBER-1原位杂交的方法，对中国东北区鼻腔恶性淋巴瘤的病理标本进行了分析，发现EB病毒相关性NK细胞淋巴瘤以扁桃体、口腔、鼻腔最多。在这些鼻型NK细胞淋巴瘤中，虽然未发现P53蛋白的异常表达和磷酸化P53蛋白的高表达，但在鼻型NK细胞淋巴瘤的背景中，许多巨噬细胞表达了凋亡产物CD204，提示其退变趋势是细胞凋亡。在下一个项目中，我们检查了裂解的caspase-3的免疫组织化学，发现该免疫组织化学可以在细胞核变性之前检测到细胞在凋亡中坠落。在对中国人鼻NK细胞淋巴瘤间质细胞的分析中，在这些淋巴瘤中发现了未成熟的胸苷磷酸化酶阳性间质细胞，而在外周T细胞淋巴瘤中则发现了T细胞依赖的树突状细胞。在下一个项目中，我们检查了干细胞1 S标记的免疫组织化学是否可以应用于检测骨髓、外周血和组织中的干细胞，发现用这种方法标记人类病理标本中的组织干细胞是可能的。本项目尚不清楚是否有鼻型NK细胞淋巴瘤的先兆病变，当本项目显示鼻型NK细胞淋巴瘤在中国罕见时。在本项目期间，由于该项目是一个分析中医病理标本的国际项目，因此准备了该项目的研究伦理方面的内容。

This research-project analyzed pathology-specimens of nasal malignant lymphomas in the east-northern China by means of immunohistochemistry and EBER-1 in-situ hybridization and found more Epstein-Barr virus (EBV)-related NK-cell lymphomas in the order of tonsil, oral cavity and nose. In these nasal NK-cell lymphomas, expression of CD204, a receptor for apoptosis products, was noted in many macrophages in the background of the nasal NK-cell lymphomas, suggesting that degenerative tendency was that of apoptosis, although abnormal expression of p53 protein and high expression of phosphorylated p53 protein were not detected. For the next project, we checked immunohistochemistry of cleaved caspase-3 and found that the immunohistochemistry can detect cells falling in apoptosis before nuclear degeneration. In analysis of stromal cells in the background Chinese nasal NK-cell lymphomas, immature thymidine phosphorylase-positive stromal cells were noted in these lymphomas, when T-cell-dependent dendritic cells are seen in peripheral T-cell lymphomas. For the next project, we checked whether immunohistochemistry of stem cell 1 s markers can be applied to detect stem cells in the bone marrow, peripheral blood and the tissues and found that it is possible by this method to label tissue stem cells in human pathology-specimens. It was unknown in this project whether there were premonitory lesions of nasal NK-cell lymphomas, when it was shown in this project that nasal type NK-cell lymphomas were rare in China. In the period of this project, research ethical aspects of this project were prepared, since this project is an international one analyzing pathology specimens in the Chinese medicine.