Pathelogical and nualecular biological study of Hepatitis C Virus infection

采用巢式聚合酶链反应和酶联免疫吸附法测定了8只实验感染丙型肝炎病毒的黑猩猩的血清样本中丙型肝炎病毒RNA与丙型肝炎病毒相关抗体（针对假定的衣壳蛋白和C-100抗体的抗体）之间的关系。聚合酶链反应数据显示出三种不同的模式：第一组（1组）是急性溶解型肝炎，伴有短暂的HCV RNA出现（2例）。第二组（2组）为慢性肝炎伴持续性丙型肝炎病毒RNA阳性（4例），第三组（3组）为慢性肝炎伴间断性丙型肝炎病毒RNA阳性（2例）。在8只动物中，有4只在接种后1周的血清中首次检测到丙型肝炎病毒RNA。虽然在所有受感染的黑猩猩中都检测到血清HCV RNA，但其中两只仅对假定的衣壳蛋白抗体呈阳性，而两只仅对C-100抗原抗体呈阳性。8例患者中有4例，针对衣壳蛋白的抗体出现时间早于针对C-100抗原的抗体，在谷氨酸丙酮酸转氨酶升高之前或同时检测到，即使在康复后仍长时间保持阳性。8只动物中有6只（75%）在接种1年后仍呈丙型肝炎病毒RNA阳性，提示丙型肝炎病毒感染发展为慢性携带者状态的风险很高。此外，血清抗体滴度与丙型肝炎病毒感染滴度之间似乎没有很好的相关性。

The relationship between hepatitis C virus RNA and hepatitis C virus-associated antibodies (antibody against the putative capsid protein and C-100 antibody) was determined by nested polymerase chain reaction and enzyme-linked immunosorbent assay in serial serum samples obtained from eight chimpanzees experimentally infected with hepatitis C virus. Three different patterns emerged from the polymerase chain reaction data : the first (group 1) was acute resolving hepatitis with transient appearance of HCV RNA (two cases) . The second (group 2) had chronic hepatitis with persistent hepatitis C virus RNA positivity (four cases) and the third (group 3) had chronic hepatitis with intermittent appearance of hepatitis C virus RNA (two cases) . In four of eight animals, hepatitis C virus RNA was first detectable in serum 1 wk after inoculation. Although serum HCV RNA was detected in all infected chimpanzees, two were positive only for antibody against the putative capsid protein, whereas two were positive only for antibody to C-100 antigen. In four of eight cases, antibody against the putative capsid protein appeared earlier than did antibody to C-100 antigen, was detected just before or coincident with rising glutamate pyruvate transminase values and remained positive for a long time even after recovery. Six of eight animals (75%) were still hepatitis C virus RNA positive 1 yr after inoculation, suggesting that the risk of development of the chronic carrier state is high in hepatitis C virus infection. Furthermore, there did not appear to be a good correlation between antibody titer in serum and hepatitis C virus infectivity titer.