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Pathological and molecular-based virological studies of TTV and s-TTV infection

TTV 和 s-TTV 感染的病理学和分子病毒学研究

基本信息

批准号：
12670223
负责人：
ABE Kenji
金额：
$ 0.77万
依托单位：
National Institute of Infectious Diseases
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670223/
关键词：
TTV Simian TTV (s-TTV)TTV DNA TTV particle Molecular epidemiology ヒトDNAウイルス Simian TTV(s-TTV)肝疾患ウイルス粒子 Simian TTV s-TTV 霊長類チシパンジー PCR

项目摘要

1) Detection of TTV DNA in body fluids :TTV DNA was detected in the saliva of 7 (7/10 ; 70%) individuals and the semen of 6 (6/10 : 60%) individuals, respectively. In addition, TTV DNA was also detected frequently (8/16 ; 50%) in bile juice, suggesting a fecal-oral TTV transmission route.2) Observation of viral particles :Under the electron microscopic examination, viral-like particles (VLP) with 20-30nm in diameter were found in bile juice from a patient infected with TTV. VLPs were found in fractions with a density of 1.24-1.28/ml in continuous gradient of CsCl.3) Infection of simian TTV (s-TTV) to humans :s-TTV DNA was determined by nested PCR using s-TTV-specific primers designed from untranslated region of s-TTV genome. s-TTV DNA sequence was detected in 3 of 200 (1.5%) healthy adults. On the other hand, s-TTV DNA was detected in 18 of 260 (6.9%) Japanese patients with liver diseases (chronic hepatitis 10/217 ; 4.6%, cirrhosis 4/28 ; 142%, hepatocellular carcinoma 4/12; 33.3%). 55.5% (10/18) of patients were positive for s-TTV alone. s-TTV DNA was detected in 10/151 (6.6%) patients with liver diseases of unknown etiology. Nucleotide sequence analyses in 20 patients confirmed that these PCR products were derived from s-TTV genome sequences and nearly 85% identical to those of s-TTV prototypes from chimpanzees. Phylogenetic analysis demonstrated that all s-TTV isolates from humans were distinguished clearly from the human TTV isolates. Furthermore, s-TTV in humans was classified into 2 different genotypes as well as simians.

1)体液中TTVDNA的检测：7例（7/10 ; 70%）个体的唾液和6例（6/10：60%）个体的精液中分别检测到TTVDNA。2）病毒颗粒的观察：在1例TTV感染者的胆汁中发现直径为20- 30 nm的病毒样颗粒（viral-like particles，VLP）。3）猴TTV（s-TTV）对人的感染：用s-TTV基因组非翻译区设计的特异性引物，通过巢式PCR检测s-TTV DNA。200例健康成人中检出s-TTV DNA序列3例（1.5%）。另一方面，在260例日本肝病患者中有18例（6.9%）检测到s-TTV DNA（慢性肝炎10/217 ; 4.6%，肝硬化4/28 ; 142%，肝细胞癌4/12; 33.3%）。单纯s-TTV阳性率为55.5%（10/18）。151例不明原因肝病患者中检出s-TTV DNA 10例（6.6%）。对20名患者的核苷酸序列分析证实，这些PCR产物源自s-TTV基因组序列，与黑猩猩s-TTV原型的序列几乎85%相同。系统发育分析表明，所有的s-TTV分离株从人类TTV分离明确区分。此外，人类和猿猴中的s-TTV被分为2种不同的基因型。