Study of simian TT virus (s-TTV) infection and its pathogenesis to humans

猿猴TT病毒（s-TTV）感染及其对人类发病机制的研究

• 批准号: 14570209
• 负责人: ABE Kenji
• 金额: $ 2.37万
• 依托单位: National Institute of Infectious Diseases
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570209/
• 关键词: TT virus (TTV); simian TTV (s-TTV); molecular epidemiology; zoonosis

Recently, we identified TTV isolates from nonhuman primates and named them simian TTV (s-TTV). To investigate the prevalence of s-TTV in humans, we examined sera from healthy individuals and patients with liver diseases in Japan for the presence of s-TTV DNA by PCR assay. s-TTV DNA was determined by nested PCR using s-TTV-specific primers designed from untranslated region of s-TTV genome. s-TTV DNA sequence was detected in 3 of 200 (1.5%) healthy adults but none of 48 infants without liver disease. On the other hand, s-TTV DNA was detected in 30 of 287 (10.5%) Japanese patients with liver disease. s-TTV coinfection with hepatitis B virus and hepatitis C virus were present in 16.7% and 30% of these patients, respectively, while 53.3% of patients were positive for s-TTV alone. Nucleotide sequence analyses in 20 patients confirmed that these PCR products were derived from s-TTV genome sequences and nearly 85% identical to those of s-TTV prototypes from chimpanzees. Phylogenetic analysis demonstrated that all s-TTV isolates from humans were distinguished clearly from the human TTV isolates. Furthermore, s-TTV in humans was classified into 2 different genotypes as well as simians. Our results indicate that generally 10.5% of Japanese patients with liver diseases were infected with s-TTV. The routes of s-TTV transmission from animal to human require clarification.

最近，我们从非人灵长类动物中分离到TTV，并将其命名为猿猴TTV（s-TTV）。为了研究s-TTV在人类中的流行情况，我们通过PCR检测法检测了日本健康个体和肝病患者血清中s-TTV DNA的存在。根据s-TTV基因组非翻译区设计特异性引物，通过巢式PCR检测s-TTV DNA。200例健康成人中3例（1.5%）检出s-TTV DNA序列，48例无肝病的婴儿中未检出s-TTV DNA序列。另一方面，在287例日本肝病患者中有30例（10.5%）检测到s-TTV DNA。s-TTV与B型肝炎病毒和丙型肝炎病毒的合并感染分别占16.7%和30%，而53.3%的患者单独s-TTV阳性。20例患者的核苷酸序列分析证实，这些PCR产物来自s-TTV基因组序列和近85%相同的s-TTV原型从黑猩猩。系统发育分析表明，所有的s-TTV分离株从人类TTV分离明确区分。此外，人类和猿猴中的s-TTV被分为2种不同的基因型。我们的研究结果表明，一般10.5%的日本肝病患者感染了s-TTV。s-TTV从动物到人类的传播途径需要澄清。

项目成果

Xin Ding, Hongxi Gu, Zhao-Hua Zhong, Xu Zilong, Huy Thien-Tuan Tran, Yohko Iwaki, Tian-Cheng Li, Tetsutaro Sata, Kenji Abe: "Molecular epidemiology of hepatitis viruses and genotypic distribution of hepatitis B and C viruses in Harbin, China."Japanese Jou

Xin Ding，Hongxi Gu，Zhao-Huazhong，Xu Zilong，Huy Thien-Tuan Tran，Yohko Iwaki，Tian-Cheng Li，Tetsutaro Sata，Kenji Abe：“肝炎病毒的分子流行病学以及乙型和丙型肝炎病毒的基因型分布

Xin Ding, et al.: "Geographic characterization of hepatitis virus infections, genotyping of hepatitis B virus and p53 mutation in hepatocellular carcinoma analyzed by in situ detection of viral genomes from carcinoma tissues"(In press).

Xin Ding 等人：“通过原位检测癌组织中的病毒基因组分析肝炎病毒感染的地理特征、乙型肝炎病毒的基因分型和肝细胞癌中的 p53 突变”（正在出版）。

9.Kenji Abe, Tetsuya Kiuchi, Naoto Aiba, Huy Thien-Tuan Tran, Xin Ding, Mari Yamaguchi, Tetsutaro Sata, Koichi Tanaka: "Complete nucleotide sequence of hepatitis B virus isolated from two infants with fulminant hepatitis."Japanese Journal of Infectious Di

9.Kenji Abe、Tetsuya Kiuchi、Naoto Aiba、Huy Thien-Tuan Tran、Xin Ding、Mari Yamaguchi、Tetsutaro Sata、Koichi Tanaka：“从两名暴发性肝炎婴儿中分离出乙型肝炎病毒的完整核苷酸序列。”日本传染病杂志

Xin Ding, Tian-Cheng Li, Shigeki Hayashi, Naohiko Masaki, Tran TT Huy, Makoto Hirano, Mari Yamaguchi, Mitsugu Usui, Naokazu Takeda, Kenji Abe: "Present state of hepatitis E virus epidemiology in Tokyo, Japan."Hepatology Research. 27. 169-173 (2003)

Xin Ding、Tian-Cheng Li、Shigeki Hayashi、Naohiko Masaki、Tran TT Huy、Makoto Hirano、Mari Yamaguchi、Mitsugu Usui、Naokazu Takeda、Kenji Abe：“日本东京戊型肝炎病毒流行病学现状。”肝病学研究。

Tran TT Huy, Kenji Abe: "Review : Molecular epidemiology of hepatitis B and C virus infections in Asia."Pediatric International. 46. 223-230 (2004)

Tran TT Huy、Kenji Abe：“评论：亚洲乙型和丙型肝炎病毒感染的分子流行病学。”国际儿科。