Geographic pathology of HBV-related hepatitis and liver cancers based on characterization of HBV genome variant

(1)42例患儿肝细胞癌组织中HBVDNA阳性36例(86%)。令人惊讶的是，在接受检查的30例肝癌中，有27例(90%)发现了非常高的HBVPre-S缺失突变。有趣的是，前S2突变在所有20例前S2突变(100%)中都出现了4-57位的缺失。我们在台湾原住民乙肝病毒携带者中发现了一种新的HBVD10亚型。(3)乙肝病毒感染可能与中国北部地区肝内胆管细胞癌的发病有关。胆管细胞癌的高病死率加剧了进一步研究的需要，以了解乙肝病毒感染的确切机制和肝细胞癌的发展。

(1) HBV DNA in HCC tissues from children patients was detected in 36 of 42 (86%) cases tested. Surprisingly, very high prevalence of HBV pre-S deletion mutant was recognized in 27 of 30 (90%) HCCs examined. Interestingly, the pre-S2 mutant consistently appeared with deletion at nt 4-57 in all of the 20 cases with pre-S2 mutant (100%). This hot spot mutation in the pre-S2 was further confirmed by complete genomic sequence of HBV in a Japanese boy who eventually developed HCC.(2) We identified a novel subgenotype HBV/D10 in Taiwanese aborigine HBV carriers.(3) HBV infection appears to be associated with the pathogenesis of intrahepatic cholangiocarcinoma (ICC) in northern China. The high fatality rate of cholangiocarcinoma intensifies the need for further research to understand the precise mechanism of HBV infection and the development of ICC.