The identification of cell surface antigen of oral streptococcus for development of vaccine for oral cancer

牙菌斑中存在血管紧张素链球菌，口腔粘膜中的血管紧张素链球菌感染可能与某些鳞癌密切相关。本研究克隆了钩端螺旋体纤维连接蛋白结合蛋白基因fbp62，并探讨了该基因在钩端螺旋体感染中的致病作用。结果表明，斑点链球菌fbp62基因敲除突变株(Dfbp62)与黏膜上皮细胞的黏附能力显著降低，感染Dfbp62的小鼠死亡率和脓肿形成率明显低于野生型斑点链霉菌感染小鼠。因此，一种新的血管紧张链球菌纤维连接蛋白结合蛋白Fbp62可能成为治疗血管紧张链球菌相关口腔癌的有效候选疫苗。

Streptococcus anginosus inhabits dental plaques, and its infection in oral mucosa could be closely related with some squamous cell carcinoma. In this study for the development of vaccine, the fibronectin binding protein gene of S. anginosus, designated fbp62 was cloned, and the pathogenic involvement of Fbp62 in S. anginosusinfection was investigated. The results indicated that the adhesive ability of an fbp62knockout mutan t (Dfbp62) of S. anginosus to mucosal epithelial cells diminished significantly, and that the Dfbp62-infected mice showed considerably lower mortality and abscess formation rates, compared to the wild type S. anginosus-infected mice. Thus, a novel fibronectin binding protein of S. anginosus, Fbp62, could be an effective vaccine candidate for the S. anginosus-related oral cancer.